TY - JOUR
T1 - ABCC13, an unusual truncated ABC transporter, is highly expressed in fetal human liver
AU - Yabuuchi, Hikaru
AU - Takayanagi, Shin ichiro
AU - Yoshinaga, Keigo
AU - Taniguchi, Naoyuki
AU - Aburatani, Hiroyuki
AU - Ishikawa, Toshihisa
N1 - Funding Information:
We thank Prof. Masayuki Yamamoto (TARA Center, Tsukuba University, Tsukuba, Japan) for the helpful discussion about the functions of AML1 and GATA and Dr. Shigeki Tarui (GS platZ., Tokyo, Japan) for the generous support to perform this work. Thanks go to Mr. Kiyohiko Matsubara for his help in the promoter analysis. The present study was supported, in part, by research grants entitled “Studies on the genetic polymorphism and function of pharmacokinetics-related proteins in Japanese population” (H12-Genome-026) and “Toxicoproteomics: Expression of ABC transporter genes and drug–drug interactions” (H14-Toxico-002) from the Japanese Ministry of Health and Welfare as well as by a Grant-in-Aid for Creative Scientific Research (No. 13NP0401) and a research Grant (No. 14370754) from the Japan Society for the Promotion of Science.
PY - 2002
Y1 - 2002
N2 - In the present study, we have cloned the cDNA of ABCC13, a novel ABC transporter, from the cDNA library of adult human placenta. The ABCC13 gene spans ∼70 kb on human chromosome 21q11.2 and consists of 14 exons. The open reading frame of the ABCC13 cDNA encodes a peptide consisting of 325 amino acid residues. The amino acid sequence corresponding to putative membrane-spanning domains was remarkably similar to ABCC1, ABCC2, ABCC3, and ABCC6. The ABCC13 gene was expressed in the fetal liver at the highest level among the organs studied. While ABCC13 was expressed in the bone marrow, its expression in peripheral blood leukocytes of adult humans was much lower and no detectable levels were observed in differentiated hematopoietic cells. The expression of ABCC13 in K562 cells decreased during cell differentiation induced by TPA. These results suggest that the expression of human ABCC13 is related with hematopoiesis.
AB - In the present study, we have cloned the cDNA of ABCC13, a novel ABC transporter, from the cDNA library of adult human placenta. The ABCC13 gene spans ∼70 kb on human chromosome 21q11.2 and consists of 14 exons. The open reading frame of the ABCC13 cDNA encodes a peptide consisting of 325 amino acid residues. The amino acid sequence corresponding to putative membrane-spanning domains was remarkably similar to ABCC1, ABCC2, ABCC3, and ABCC6. The ABCC13 gene was expressed in the fetal liver at the highest level among the organs studied. While ABCC13 was expressed in the bone marrow, its expression in peripheral blood leukocytes of adult humans was much lower and no detectable levels were observed in differentiated hematopoietic cells. The expression of ABCC13 in K562 cells decreased during cell differentiation induced by TPA. These results suggest that the expression of human ABCC13 is related with hematopoiesis.
KW - ABC transporter
KW - Cell differentiation
KW - Fetal liver
KW - Hematopoiesis
KW - Human chromosome 21
KW - Leukemia cells
UR - https://www.scopus.com/pages/publications/0036434614
UR - https://www.scopus.com/pages/publications/0036434614#tab=citedBy
U2 - 10.1016/S0006-291X(02)02658-X
DO - 10.1016/S0006-291X(02)02658-X
M3 - Article
C2 - 12445816
AN - SCOPUS:0036434614
SN - 0006-291X
VL - 299
SP - 410
EP - 417
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -