Aberrant DNA methylation in ulcerative colitis without neoplasia

Fang Yu Wang, Tomiyasu Arisawa, Tomomitsu Tahara, Kazuya Takahama, Makoto Watanabe, Ichiro Hirata, Hiroshi Nakano

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background/Aims: DNA methylation has been reported to correlate with the development of colitis associated cancer. We detected the promoter methylation of estrogen receptor gene (ER), TP53, p14, p16, p21 and hMLH1 in ulcerative colitis without neoplasia. Methodology: A total of 49 specimens from 36 patients, including 36 at rectal inflammatory mucosa and 13 at terminal ileum were obtained by colonoscopic biopsies. Methylation specific polymerase chain reaction were performed to detect the methylation in promoters of the above six genes. Results: Methylation rate of ER promoter was significantly higher in the rectal mucosa than that in the ileum (76.3% vs. 46.2%, P=0.044). Moreover, ER methylation in rectal mucosa was significantly higher in relapse-remitting type compared to one attack only type cases (P=0.008), and also increased in cases longer than 7 years (P=0.036). Methylation rates of p14 or p16 were higher in rectal mucosa than those in the ileum, but the differences were not of statistic significance. Meanwhile, methylation in TP53 promoter was found in only one case, while p21 and hMLH1 methylation were negative in all cases. Conclusions: Methylation in promoters of ER, p14 and p16 occurs in rectal inflammatory mucosa without neoplasia. Examination of ER methylation in rectal mucosa may be useful for predicting cases at high risk of neoplasia.

Original languageEnglish
Pages (from-to)62-65
Number of pages4
JournalHepato-gastroenterology
Volume55
Issue number81
Publication statusPublished - 01-01-2008

Fingerprint

DNA Methylation
Ulcerative Colitis
Methylation
Mucous Membrane
Estrogen Receptors
Neoplasms
Ileum
Genes
p16 Genes
p53 Genes
Colitis
Biopsy
Recurrence
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Wang, F. Y., Arisawa, T., Tahara, T., Takahama, K., Watanabe, M., Hirata, I., & Nakano, H. (2008). Aberrant DNA methylation in ulcerative colitis without neoplasia. Hepato-gastroenterology, 55(81), 62-65.
Wang, Fang Yu ; Arisawa, Tomiyasu ; Tahara, Tomomitsu ; Takahama, Kazuya ; Watanabe, Makoto ; Hirata, Ichiro ; Nakano, Hiroshi. / Aberrant DNA methylation in ulcerative colitis without neoplasia. In: Hepato-gastroenterology. 2008 ; Vol. 55, No. 81. pp. 62-65.
@article{39b9af5f52914ffa8071e7729b28a82c,
title = "Aberrant DNA methylation in ulcerative colitis without neoplasia",
abstract = "Background/Aims: DNA methylation has been reported to correlate with the development of colitis associated cancer. We detected the promoter methylation of estrogen receptor gene (ER), TP53, p14, p16, p21 and hMLH1 in ulcerative colitis without neoplasia. Methodology: A total of 49 specimens from 36 patients, including 36 at rectal inflammatory mucosa and 13 at terminal ileum were obtained by colonoscopic biopsies. Methylation specific polymerase chain reaction were performed to detect the methylation in promoters of the above six genes. Results: Methylation rate of ER promoter was significantly higher in the rectal mucosa than that in the ileum (76.3{\%} vs. 46.2{\%}, P=0.044). Moreover, ER methylation in rectal mucosa was significantly higher in relapse-remitting type compared to one attack only type cases (P=0.008), and also increased in cases longer than 7 years (P=0.036). Methylation rates of p14 or p16 were higher in rectal mucosa than those in the ileum, but the differences were not of statistic significance. Meanwhile, methylation in TP53 promoter was found in only one case, while p21 and hMLH1 methylation were negative in all cases. Conclusions: Methylation in promoters of ER, p14 and p16 occurs in rectal inflammatory mucosa without neoplasia. Examination of ER methylation in rectal mucosa may be useful for predicting cases at high risk of neoplasia.",
author = "Wang, {Fang Yu} and Tomiyasu Arisawa and Tomomitsu Tahara and Kazuya Takahama and Makoto Watanabe and Ichiro Hirata and Hiroshi Nakano",
year = "2008",
month = "1",
day = "1",
language = "English",
volume = "55",
pages = "62--65",
journal = "Acta hepato-splenologica",
issn = "0172-6390",
publisher = "H.G.E. Update Medical Publishing Ltd.",
number = "81",

}

Wang, FY, Arisawa, T, Tahara, T, Takahama, K, Watanabe, M, Hirata, I & Nakano, H 2008, 'Aberrant DNA methylation in ulcerative colitis without neoplasia', Hepato-gastroenterology, vol. 55, no. 81, pp. 62-65.

Aberrant DNA methylation in ulcerative colitis without neoplasia. / Wang, Fang Yu; Arisawa, Tomiyasu; Tahara, Tomomitsu; Takahama, Kazuya; Watanabe, Makoto; Hirata, Ichiro; Nakano, Hiroshi.

In: Hepato-gastroenterology, Vol. 55, No. 81, 01.01.2008, p. 62-65.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Aberrant DNA methylation in ulcerative colitis without neoplasia

AU - Wang, Fang Yu

AU - Arisawa, Tomiyasu

AU - Tahara, Tomomitsu

AU - Takahama, Kazuya

AU - Watanabe, Makoto

AU - Hirata, Ichiro

AU - Nakano, Hiroshi

PY - 2008/1/1

Y1 - 2008/1/1

N2 - Background/Aims: DNA methylation has been reported to correlate with the development of colitis associated cancer. We detected the promoter methylation of estrogen receptor gene (ER), TP53, p14, p16, p21 and hMLH1 in ulcerative colitis without neoplasia. Methodology: A total of 49 specimens from 36 patients, including 36 at rectal inflammatory mucosa and 13 at terminal ileum were obtained by colonoscopic biopsies. Methylation specific polymerase chain reaction were performed to detect the methylation in promoters of the above six genes. Results: Methylation rate of ER promoter was significantly higher in the rectal mucosa than that in the ileum (76.3% vs. 46.2%, P=0.044). Moreover, ER methylation in rectal mucosa was significantly higher in relapse-remitting type compared to one attack only type cases (P=0.008), and also increased in cases longer than 7 years (P=0.036). Methylation rates of p14 or p16 were higher in rectal mucosa than those in the ileum, but the differences were not of statistic significance. Meanwhile, methylation in TP53 promoter was found in only one case, while p21 and hMLH1 methylation were negative in all cases. Conclusions: Methylation in promoters of ER, p14 and p16 occurs in rectal inflammatory mucosa without neoplasia. Examination of ER methylation in rectal mucosa may be useful for predicting cases at high risk of neoplasia.

AB - Background/Aims: DNA methylation has been reported to correlate with the development of colitis associated cancer. We detected the promoter methylation of estrogen receptor gene (ER), TP53, p14, p16, p21 and hMLH1 in ulcerative colitis without neoplasia. Methodology: A total of 49 specimens from 36 patients, including 36 at rectal inflammatory mucosa and 13 at terminal ileum were obtained by colonoscopic biopsies. Methylation specific polymerase chain reaction were performed to detect the methylation in promoters of the above six genes. Results: Methylation rate of ER promoter was significantly higher in the rectal mucosa than that in the ileum (76.3% vs. 46.2%, P=0.044). Moreover, ER methylation in rectal mucosa was significantly higher in relapse-remitting type compared to one attack only type cases (P=0.008), and also increased in cases longer than 7 years (P=0.036). Methylation rates of p14 or p16 were higher in rectal mucosa than those in the ileum, but the differences were not of statistic significance. Meanwhile, methylation in TP53 promoter was found in only one case, while p21 and hMLH1 methylation were negative in all cases. Conclusions: Methylation in promoters of ER, p14 and p16 occurs in rectal inflammatory mucosa without neoplasia. Examination of ER methylation in rectal mucosa may be useful for predicting cases at high risk of neoplasia.

UR - http://www.scopus.com/inward/record.url?scp=41549125402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41549125402&partnerID=8YFLogxK

M3 - Article

C2 - 18507080

AN - SCOPUS:41549125402

VL - 55

SP - 62

EP - 65

JO - Acta hepato-splenologica

JF - Acta hepato-splenologica

SN - 0172-6390

IS - 81

ER -

Wang FY, Arisawa T, Tahara T, Takahama K, Watanabe M, Hirata I et al. Aberrant DNA methylation in ulcerative colitis without neoplasia. Hepato-gastroenterology. 2008 Jan 1;55(81):62-65.