Aberrant glycosylation of lumican in aortic valve stenosis revealed by proteomic analysis

Hirotoshi Suzuki, Masahide Chikada, Michiyo K. Yokoyama, Manae S. Kurokawa, Takashi Ando, Hiroshi Furukawa, Mitsumi Arito, Takeshi Miyairi, Tomohiro Kato

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

To identify proteins related to the pathophysiology of aortic valve stenosis (AS), we investigated the protein profiles of AS aortic valves. Specifically, proteins were extracted from a thickened and calcified area (AS-C) and an apparently non-thickened and non-calcified area (AS-N) in an identical aortic valve leaflet in each of 6 AS patients. The proteins were then separated by 2-dimensional gel electrophoresis (2DE). Protein spots detected by 2DE were compared between the AS-C and AS-N samples. Protein spots of interest were subjected to protein identification by mass spectrometry. In total, 670 protein spots were detected by 2DE, 28 of which showed more than 1.5-fold different intensity (P < 0.05) between the AS-C and AS-N samples. Proteins were identified in 17 out of the 28 spots. Fibrinogen and lumican were identified in 9 and 3 spots, respectively. Intensity of these 12 spots was lower in the AS-C samples than in the AS-N samples. In the 1D-Western blot analysis, 4 lumican bands (80 kDa, 75 kDa, 65 kDa, and 53 kDa) were detected, of which 2 bands with 80 kDa and 75 kDa showed lower intensity in the AS-C samples than in the AS-N samples. When de-glycosylated protein samples were used in the 1D-Western blot, only a single lumican band with ~40 kDa was detected, indicating that lumican was variously glycosylated and that highly glycosylated lumican molecules were decreased in AS-C. Collectively, insufficient glycosylation of lumican in the thickened and calcified areas of AS aortic valves may be involved in the pathophysiology of AS.

Original languageEnglish
Pages (from-to)104-111
Number of pages8
JournalInternational heart journal
Volume57
Issue number1
DOIs
Publication statusPublished - 19-01-2016

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Fingerprint

Dive into the research topics of 'Aberrant glycosylation of lumican in aortic valve stenosis revealed by proteomic analysis'. Together they form a unique fingerprint.

Cite this