Abnormal cytoplasmic dyslocalisation and/or reduction of nucleophosmin protein level rarely occurs in myelodysplastic syndromes

Yuichi Ishikawa, Jinglan Xu, Gyosuke Sakashita, Takeshi Urano, Tatsuya Suzuki, Akihiro Tomita, Hitoshi Kiyoi, Shigeo Nakamura, Tomoki Naoe

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The Nucleophosmin1 (NPM1) gene located in chromosome 5q35 is affected by chromosomal translocation, mutation and deletion in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). NPM1 haploinsufficiency reportedly causes MDS-like disorders in knockout mice. Here, we studied mRNA and protein expression in bone marrow (BM) samples from 36 patients with MDS. The NPM1 expression levels of mRNA and protein were not related to chromosome 5 abnormalities and were almost the same as those in normal BM and AML cells. However, the protein levels in AML cells with NPM1 mutations were slightly lower than in those without mutation. Immunochemical studies showed no difference in the staining intensity and subcellular localisation between MDS and normal BM cells. It was concluded that abnormal cytoplasmic localisation and/or significant reduction of NPM1 protein level rarely occurs in MDS. The increase in the number of nuclear NPM1-positive cells may be related to the progression of MDS.

Original languageEnglish
Pages (from-to)2359-2364
Number of pages6
JournalLeukemia and Lymphoma
Volume49
Issue number12
DOIs
Publication statusPublished - 16-12-2008
Externally publishedYes

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Myelodysplastic Syndromes
Acute Myeloid Leukemia
Proteins
Myeloid Cells
Bone Marrow
Haploinsufficiency
Genetic Translocation
Chromosomes, Human, Pair 5
Messenger RNA
Mutation
Sequence Deletion
Knockout Mice
Chromosome Aberrations
Bone Marrow Cells
nucleophosmin
Chromosomes
Staining and Labeling
Genes

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Ishikawa, Yuichi ; Xu, Jinglan ; Sakashita, Gyosuke ; Urano, Takeshi ; Suzuki, Tatsuya ; Tomita, Akihiro ; Kiyoi, Hitoshi ; Nakamura, Shigeo ; Naoe, Tomoki. / Abnormal cytoplasmic dyslocalisation and/or reduction of nucleophosmin protein level rarely occurs in myelodysplastic syndromes. In: Leukemia and Lymphoma. 2008 ; Vol. 49, No. 12. pp. 2359-2364.
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Abnormal cytoplasmic dyslocalisation and/or reduction of nucleophosmin protein level rarely occurs in myelodysplastic syndromes. / Ishikawa, Yuichi; Xu, Jinglan; Sakashita, Gyosuke; Urano, Takeshi; Suzuki, Tatsuya; Tomita, Akihiro; Kiyoi, Hitoshi; Nakamura, Shigeo; Naoe, Tomoki.

In: Leukemia and Lymphoma, Vol. 49, No. 12, 16.12.2008, p. 2359-2364.

Research output: Contribution to journalArticle

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AU - Ishikawa, Yuichi

AU - Xu, Jinglan

AU - Sakashita, Gyosuke

AU - Urano, Takeshi

AU - Suzuki, Tatsuya

AU - Tomita, Akihiro

AU - Kiyoi, Hitoshi

AU - Nakamura, Shigeo

AU - Naoe, Tomoki

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