TY - JOUR
T1 - Abnormal cytoplasmic dyslocalisation and/or reduction of nucleophosmin protein level rarely occurs in myelodysplastic syndromes
AU - Ishikawa, Yuichi
AU - Xu, Jinglan
AU - Sakashita, Gyosuke
AU - Urano, Takeshi
AU - Suzuki, Tatsuya
AU - Tomita, Akihiro
AU - Kiyoi, Hitoshi
AU - Nakamura, Shigeo
AU - Naoe, Tomoki
N1 - Funding Information:
This study was supported by Grant-in-Aids from the National Institute of Biomedical Innovation (to T.N.), for Scientific Research (to T.N., T.U. and G.S.) and the Japan Leukemia Research Fund (to T.U.).
PY - 2008
Y1 - 2008
N2 - The Nucleophosmin1 (NPM1) gene located in chromosome 5q35 is affected by chromosomal translocation, mutation and deletion in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). NPM1 haploinsufficiency reportedly causes MDS-like disorders in knockout mice. Here, we studied mRNA and protein expression in bone marrow (BM) samples from 36 patients with MDS. The NPM1 expression levels of mRNA and protein were not related to chromosome 5 abnormalities and were almost the same as those in normal BM and AML cells. However, the protein levels in AML cells with NPM1 mutations were slightly lower than in those without mutation. Immunochemical studies showed no difference in the staining intensity and subcellular localisation between MDS and normal BM cells. It was concluded that abnormal cytoplasmic localisation and/or significant reduction of NPM1 protein level rarely occurs in MDS. The increase in the number of nuclear NPM1-positive cells may be related to the progression of MDS.
AB - The Nucleophosmin1 (NPM1) gene located in chromosome 5q35 is affected by chromosomal translocation, mutation and deletion in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). NPM1 haploinsufficiency reportedly causes MDS-like disorders in knockout mice. Here, we studied mRNA and protein expression in bone marrow (BM) samples from 36 patients with MDS. The NPM1 expression levels of mRNA and protein were not related to chromosome 5 abnormalities and were almost the same as those in normal BM and AML cells. However, the protein levels in AML cells with NPM1 mutations were slightly lower than in those without mutation. Immunochemical studies showed no difference in the staining intensity and subcellular localisation between MDS and normal BM cells. It was concluded that abnormal cytoplasmic localisation and/or significant reduction of NPM1 protein level rarely occurs in MDS. The increase in the number of nuclear NPM1-positive cells may be related to the progression of MDS.
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U2 - 10.1080/10428190802541815
DO - 10.1080/10428190802541815
M3 - Article
C2 - 19052985
AN - SCOPUS:57449085910
SN - 1042-8194
VL - 49
SP - 2359
EP - 2364
JO - Leukemia and Lymphoma
JF - Leukemia and Lymphoma
IS - 12
ER -
