Abnormal synaptic transmission in the olfactory bulb of Fyn-kinase- deficient mice

Hiromasa Kitazawa, Takeshi Yagi, Tsuyoshi Miyakawa, Hiroaki Niki, Nobufumi Kawai

Research output: Contribution to journalArticle

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Abstract

We studied synaptic transmission in the granule cells in the olfactory bulb of the homozygous Fyn (a nonreceptor type tyrosine kinase)-deficient (fyn2/fyn2) and heterozygous Fyn-deficient (+/fyn2) mice by using slice preparations from the olfactory bulb. Stimulation to the lateral olfactory tract and/or centrifugal fibers to the olfactory bulb evoked field excitatory postsynaptic potentials (fEPSPs) in the granule cells. In +/fyn2 mice, fEPSPs were augmented by bicuculline, a γ-aminobutyric acid (GABA(A)) antagonist and picrotoxin, whereas fEPSPs in fyn2/fyn2 mice were much less sensitive to bicuculline and picrotoxin. Application of D-2-amino-5- phosphonopentanoic acid had no effect but 6-cyano-7-nitroquinoxaline-2,3- dione produced almost complete block of fEPSPs in both +/fyn2 mice and fyn2/fyn2 mice. (1S, 3R)-1-aminocyclo-pentane-1.3-dicarboxylate, an agonist of metabotropic glutamate receptors caused a similar depression of fEPSPs in both +/fyn2 and fyn2/fyn2 mice. In +/fyn2 mice tetanic stimulation to the lateral olfactory tract and/or centrifugal fibers induced N-methyl-D- aspartate (NMDA)-dependent long-term potentiation (LTP) of fEPSPs, whereas LTP was impaired in fyn2/fyn2 mice. Our results demonstrate altered functions of GABA(A) and NMDA receptors in the olfactory system of Fyn deficient mice.

Original languageEnglish
Pages (from-to)137-142
Number of pages6
JournalJournal of Neurophysiology
Volume79
Issue number1
DOIs
Publication statusPublished - 01-01-1998
Externally publishedYes

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Olfactory Bulb
Synaptic Transmission
Phosphotransferases
Excitatory Postsynaptic Potentials
Picrotoxin
Bicuculline
Long-Term Potentiation
6-Cyano-7-nitroquinoxaline-2,3-dione
2-Amino-5-phosphonovalerate
Aminobutyrates
GABA-A Receptor Antagonists
Metabotropic Glutamate Receptors
N-Methylaspartate
N-Methyl-D-Aspartate Receptors
Protein-Tyrosine Kinases
gamma-Aminobutyric Acid

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

Cite this

Kitazawa, Hiromasa ; Yagi, Takeshi ; Miyakawa, Tsuyoshi ; Niki, Hiroaki ; Kawai, Nobufumi. / Abnormal synaptic transmission in the olfactory bulb of Fyn-kinase- deficient mice. In: Journal of Neurophysiology. 1998 ; Vol. 79, No. 1. pp. 137-142.
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Abnormal synaptic transmission in the olfactory bulb of Fyn-kinase- deficient mice. / Kitazawa, Hiromasa; Yagi, Takeshi; Miyakawa, Tsuyoshi; Niki, Hiroaki; Kawai, Nobufumi.

In: Journal of Neurophysiology, Vol. 79, No. 1, 01.01.1998, p. 137-142.

Research output: Contribution to journalArticle

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