Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis

Nozomu Yoshioka, Shinji Miyata, Atsushi Tamada, Yumi Watanabe, Asami Kawasaki, Hiroshi Kitagawa, Keizo Takao, Tsuyoshi Miyakawa, Kosei Takeuchi, Michihiro Igarashi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Chondroitin sulfate (CS) is an important glycosaminoglycan and is mainly found in the extracellular matrix as CS proteoglycans. In the brain, CS proteoglycans are highly concentrated in perineuronal nets (PNNs), which surround synapses and modulate their functions. To investigate the importance of CS, we produced and precisely examined mice that were deficient in the CS synthesizing enzyme, CSGalNAcT1 (T1KO). Biochemical analysis of T1KO revealed that loss of this enzyme reduced the amount of CS by approximately 50% in various brain regions. The amount of CS in PNNs was also diminished in T1KO compared to wild-type mice, although the amount of a major CS proteoglycan core protein, aggrecan, was not changed. In T1KO, we observed abnormalities in several behavioral tests, including the open-field test, acoustic startle response, and social preference. These results suggest that T1 is important for plasticity, probably due to regulation of CS-dependent PNNs, and that T1KO is a good model for investigation of PNNs.

Original languageEnglish
Article number47
JournalMolecular brain
Volume10
Issue number1
DOIs
Publication statusPublished - 05-10-2017

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Chondroitin Sulfates
Chondroitin Sulfate Proteoglycans
Enzymes
Startle Reflex
Aggrecans
Brain
Glycosaminoglycans
Acoustics
Synapses
Extracellular Matrix
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Yoshioka, N., Miyata, S., Tamada, A., Watanabe, Y., Kawasaki, A., Kitagawa, H., ... Igarashi, M. (2017). Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis. Molecular brain, 10(1), [47]. https://doi.org/10.1186/s13041-017-0328-5
Yoshioka, Nozomu ; Miyata, Shinji ; Tamada, Atsushi ; Watanabe, Yumi ; Kawasaki, Asami ; Kitagawa, Hiroshi ; Takao, Keizo ; Miyakawa, Tsuyoshi ; Takeuchi, Kosei ; Igarashi, Michihiro. / Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis. In: Molecular brain. 2017 ; Vol. 10, No. 1.
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abstract = "Chondroitin sulfate (CS) is an important glycosaminoglycan and is mainly found in the extracellular matrix as CS proteoglycans. In the brain, CS proteoglycans are highly concentrated in perineuronal nets (PNNs), which surround synapses and modulate their functions. To investigate the importance of CS, we produced and precisely examined mice that were deficient in the CS synthesizing enzyme, CSGalNAcT1 (T1KO). Biochemical analysis of T1KO revealed that loss of this enzyme reduced the amount of CS by approximately 50{\%} in various brain regions. The amount of CS in PNNs was also diminished in T1KO compared to wild-type mice, although the amount of a major CS proteoglycan core protein, aggrecan, was not changed. In T1KO, we observed abnormalities in several behavioral tests, including the open-field test, acoustic startle response, and social preference. These results suggest that T1 is important for plasticity, probably due to regulation of CS-dependent PNNs, and that T1KO is a good model for investigation of PNNs.",
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Yoshioka, N, Miyata, S, Tamada, A, Watanabe, Y, Kawasaki, A, Kitagawa, H, Takao, K, Miyakawa, T, Takeuchi, K & Igarashi, M 2017, 'Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis', Molecular brain, vol. 10, no. 1, 47. https://doi.org/10.1186/s13041-017-0328-5

Abnormalities in perineuronal nets and behavior in mice lacking CSGalNAcT1, a key enzyme in chondroitin sulfate synthesis. / Yoshioka, Nozomu; Miyata, Shinji; Tamada, Atsushi; Watanabe, Yumi; Kawasaki, Asami; Kitagawa, Hiroshi; Takao, Keizo; Miyakawa, Tsuyoshi; Takeuchi, Kosei; Igarashi, Michihiro.

In: Molecular brain, Vol. 10, No. 1, 47, 05.10.2017.

Research output: Contribution to journalArticle

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AU - Watanabe, Yumi

AU - Kawasaki, Asami

AU - Kitagawa, Hiroshi

AU - Takao, Keizo

AU - Miyakawa, Tsuyoshi

AU - Takeuchi, Kosei

AU - Igarashi, Michihiro

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