Absence of carboxypeptidase E leads to adult hippocampal neuronal degeneration and memory deficits

Alicja Woronowicz, Hisatsugu Koshimizu, Su Youne Chang, Niamh X. Cawley, Joanna M. Hill, Ramona M. Rodriguiz, Daniel Abebe, Caroline Dorfman, Vladimir Senatorov, An Zhou, Zhi Gang Xiong, William C. Wetsel, Y. Peng Loh

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Molecules that govern the formation, integrity, and function of the hippocampus remain an important area of investigation. Here we show that absence of the proneuropeptide processing enzyme, carboxypeptidase E (CPE) in CPE knock-out (KO) mice had a profound effect on memory, synaptic physiology, and the cytoarchitecture of the hippocampus in these animals. Adult CPE-KO mice displayed deficits in memory consolidation as revealed by the water-maze, object preference, and social transmission of food preference tests. These mice also showed no evoked long-term potentiation. Additionally, CPE-KO mice at 4 weeks of age and older, but not at 3 weeks of age, exhibited marked degeneration specifically of the pyramidal neurons in the hippocampal CA3 region which normally expresses high levels of CPE. Immunohistochemistry revealed that the neuronal marker, NeuN, was reduced, while the glial marker, GFAP, was increased, characteristic of gliosis in the CA3 area of CPE-KO mice. Calbindin staining indicated early termination of the mossy fibers before reaching the CA1 region in these mice. Thus, absence of CPE leads to degeneration of the CA3 neurons and perturbation of the cytoarchitecture of the hippocampus. Ex vivo studies showed that overexpression of CPE in cultured hippocampal neurons protected them against H2O2 oxidative-stress induced cell death. These findings taken together indicate that CPE is essential for the survival of adult hippocampal CA3 neurons to maintain normal cognitive function.

Original languageEnglish
Pages (from-to)1051-1063
Number of pages13
JournalHippocampus
Volume18
Issue number10
DOIs
Publication statusPublished - 07-11-2008

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Carboxypeptidase H
Memory Disorders
Knockout Mice
Hippocampus
Hippocampal CA3 Region
Neurons
Calbindins
Food Preferences
Nerve Degeneration
Gliosis
Long-Term Potentiation
Pyramidal Cells
Neuroglia
Cognition
Oxidative Stress
Cell Death
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Cognitive Neuroscience

Cite this

Woronowicz, A., Koshimizu, H., Chang, S. Y., Cawley, N. X., Hill, J. M., Rodriguiz, R. M., ... Loh, Y. P. (2008). Absence of carboxypeptidase E leads to adult hippocampal neuronal degeneration and memory deficits. Hippocampus, 18(10), 1051-1063. https://doi.org/10.1002/hipo.20462
Woronowicz, Alicja ; Koshimizu, Hisatsugu ; Chang, Su Youne ; Cawley, Niamh X. ; Hill, Joanna M. ; Rodriguiz, Ramona M. ; Abebe, Daniel ; Dorfman, Caroline ; Senatorov, Vladimir ; Zhou, An ; Xiong, Zhi Gang ; Wetsel, William C. ; Loh, Y. Peng. / Absence of carboxypeptidase E leads to adult hippocampal neuronal degeneration and memory deficits. In: Hippocampus. 2008 ; Vol. 18, No. 10. pp. 1051-1063.
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Woronowicz, A, Koshimizu, H, Chang, SY, Cawley, NX, Hill, JM, Rodriguiz, RM, Abebe, D, Dorfman, C, Senatorov, V, Zhou, A, Xiong, ZG, Wetsel, WC & Loh, YP 2008, 'Absence of carboxypeptidase E leads to adult hippocampal neuronal degeneration and memory deficits', Hippocampus, vol. 18, no. 10, pp. 1051-1063. https://doi.org/10.1002/hipo.20462

Absence of carboxypeptidase E leads to adult hippocampal neuronal degeneration and memory deficits. / Woronowicz, Alicja; Koshimizu, Hisatsugu; Chang, Su Youne; Cawley, Niamh X.; Hill, Joanna M.; Rodriguiz, Ramona M.; Abebe, Daniel; Dorfman, Caroline; Senatorov, Vladimir; Zhou, An; Xiong, Zhi Gang; Wetsel, William C.; Loh, Y. Peng.

In: Hippocampus, Vol. 18, No. 10, 07.11.2008, p. 1051-1063.

Research output: Contribution to journalArticle

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T1 - Absence of carboxypeptidase E leads to adult hippocampal neuronal degeneration and memory deficits

AU - Woronowicz, Alicja

AU - Koshimizu, Hisatsugu

AU - Chang, Su Youne

AU - Cawley, Niamh X.

AU - Hill, Joanna M.

AU - Rodriguiz, Ramona M.

AU - Abebe, Daniel

AU - Dorfman, Caroline

AU - Senatorov, Vladimir

AU - Zhou, An

AU - Xiong, Zhi Gang

AU - Wetsel, William C.

AU - Loh, Y. Peng

PY - 2008/11/7

Y1 - 2008/11/7

N2 - Molecules that govern the formation, integrity, and function of the hippocampus remain an important area of investigation. Here we show that absence of the proneuropeptide processing enzyme, carboxypeptidase E (CPE) in CPE knock-out (KO) mice had a profound effect on memory, synaptic physiology, and the cytoarchitecture of the hippocampus in these animals. Adult CPE-KO mice displayed deficits in memory consolidation as revealed by the water-maze, object preference, and social transmission of food preference tests. These mice also showed no evoked long-term potentiation. Additionally, CPE-KO mice at 4 weeks of age and older, but not at 3 weeks of age, exhibited marked degeneration specifically of the pyramidal neurons in the hippocampal CA3 region which normally expresses high levels of CPE. Immunohistochemistry revealed that the neuronal marker, NeuN, was reduced, while the glial marker, GFAP, was increased, characteristic of gliosis in the CA3 area of CPE-KO mice. Calbindin staining indicated early termination of the mossy fibers before reaching the CA1 region in these mice. Thus, absence of CPE leads to degeneration of the CA3 neurons and perturbation of the cytoarchitecture of the hippocampus. Ex vivo studies showed that overexpression of CPE in cultured hippocampal neurons protected them against H2O2 oxidative-stress induced cell death. These findings taken together indicate that CPE is essential for the survival of adult hippocampal CA3 neurons to maintain normal cognitive function.

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