TY - JOUR
T1 - Absence of common polymorphisms of toll like receptor 4 (TLR4)
T2 - Asp299Gly, Thr399Ile in patients with gastroduodenal diseases in Japan
AU - Tahara, Tomomitsu
AU - Arisawa, Tomiyasu
AU - Shibata, Tomoyuki
AU - Hirata, Ichiro
AU - Nakano, Hiroshi
PY - 2007/1
Y1 - 2007/1
N2 - Host genetic factors may play a key role in determining the long-term outcome of the Helicobacter pylori (H. pylori) infection. Toll-like receptor 4 (TLR4) mediated recognition of lipo-polysaccharide (LPS) is required for efficient recognition of gram-negative bacterial infections. The aim of this study is to investigate the effects of common polymorphisms of TLR4 Asp299Gly, Thr399Ile in patients with gastroduodenal diseases in Japanese population. The study was performed in 149 gastric cancer (GC) cases (mean age 64.0 ± 12.4, M:F = 109:40) and 94 patients without evidence of GC (mean age 64.1 ± 12.3, M:F = 65:25) as the control group. TLR4 Asp299Gly, Thr399Ile were determined by Polymerase chain reaction-length of polymorphisms (PCR-RFLP) in all the patients. Asp299Gly, Thr399Ile were not detected in all 243 patients enrolled in this study. In conclusion, our data suggest that TLR4 Asp299Gly, Thr399Ile are very rare in Japanese population and thus they may not be a important factor in determining the outcome of H. pylori infected individuals in Japan.
AB - Host genetic factors may play a key role in determining the long-term outcome of the Helicobacter pylori (H. pylori) infection. Toll-like receptor 4 (TLR4) mediated recognition of lipo-polysaccharide (LPS) is required for efficient recognition of gram-negative bacterial infections. The aim of this study is to investigate the effects of common polymorphisms of TLR4 Asp299Gly, Thr399Ile in patients with gastroduodenal diseases in Japanese population. The study was performed in 149 gastric cancer (GC) cases (mean age 64.0 ± 12.4, M:F = 109:40) and 94 patients without evidence of GC (mean age 64.1 ± 12.3, M:F = 65:25) as the control group. TLR4 Asp299Gly, Thr399Ile were determined by Polymerase chain reaction-length of polymorphisms (PCR-RFLP) in all the patients. Asp299Gly, Thr399Ile were not detected in all 243 patients enrolled in this study. In conclusion, our data suggest that TLR4 Asp299Gly, Thr399Ile are very rare in Japanese population and thus they may not be a important factor in determining the outcome of H. pylori infected individuals in Japan.
KW - Gastric cancer
KW - Helicobacter pylori
KW - Polymorphism
KW - Toll-like receptor 4
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U2 - 10.3164/jcbn.40.62
DO - 10.3164/jcbn.40.62
M3 - Article
C2 - 18437214
AN - SCOPUS:33947380588
SN - 0912-0009
VL - 40
SP - 62
EP - 65
JO - Journal of Clinical Biochemistry and Nutrition
JF - Journal of Clinical Biochemistry and Nutrition
IS - 1
ER -
