Absence of primary cilia in cell cycle-arrested human breast cancer cells

Kentaro Nobutani, Yohei Shimono, Midori Yoshida, Kiyohito Mizutani, Akihiro Minami, Seishi Kono, Toru Mukohara, Takashi Yamasaki, Tomoo Itoh, Shintaro Takao, Hironobu Minami, Takeshi Azuma, Yoshimi Takai

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Previous studies using cultured cells showed that primary cilia are present in quiescent cells, but are absent in proliferating cells. We studied here the relationship between the presence or absence of primary cilia and the cell cycle arrest of normal epithelial cells and cancer cells in the human normal breast and breast cancer tissues. In normal breast tissues, although most epithelial cells were nonproliferating as estimated by the immunofluorescence staining of the proliferation marker Ki-67, primary cilia were present only in 20-40% of the epithelial cells. In breast cancer tissues, primary cilia were not observed in any of the breast cancer cells. Furthermore, primary cilia were hardly observed in the nonproliferating cancer cells in the orthotopic and metastatic human breast cancer xenograft tumors in mice. These results indicate that the absence of primary cilia does not necessarily represent the proliferating phases of normal epithelial cells and cancer cells.

Original languageEnglish
Pages (from-to)141-152
Number of pages12
JournalGenes to Cells
Volume19
Issue number2
DOIs
Publication statusPublished - 02-2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

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