ACBD3-mediated recruitment of PI4KB to picornavirus RNA replication sites

Jun Sasaki, Kumiko Ishikawa, Minetaro Arita, Koki Taniguchi

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Phosphatidylinositol 4-kinase IIIÎ 2 (PI4KB) is a host factor required for genome RNA replication of enteroviruses, small non-enveloped viruses belonging to the family Picornaviridae. Here, we demonstrated that PI4KB is also essential for genome replication of another picornavirus, Aichi virus (AiV), but is recruited to the genome replication sites by a different strategy from that utilized by enteroviruses. AiV non-structural proteins, 2B, 2BC, 2C, 3A, and 3AB, interacted with a Golgi protein, acyl-coenzyme A binding domain containing 3 (ACBD3). Furthermore, we identified previously unknown interaction between ACBD3 and PI4KB, which provides a novel manner of Golgi recruitment of PI4KB. Knockdown of ACBD3 or PI4KB suppressed AiV RNA replication. The viral proteins, ACBD3, PI4KB, and phophatidylinositol-4-phosphate (PI4P) localized to the viral RNA replication sites. AiV replication and recruitment of PI4KB to the RNA replication sites were not affected by brefeldin A, in contrast to those in enterovirus infection. These results indicate that a viral protein/ACBD3/PI4KB complex is formed to synthesize PI4P at the AiV RNA replication sites and plays an essential role in viral RNA replication.

Original languageEnglish
Pages (from-to)754-766
Number of pages13
JournalEMBO Journal
Volume31
Issue number3
DOIs
Publication statusPublished - 01-02-2012

Fingerprint

Kobuvirus
Picornaviridae
Acyl Coenzyme A
Viruses
RNA
Virus Replication
Enterovirus
Viral RNA
Viral Proteins
Genome
Genes
Phosphates
Enterovirus Infections
Viral Structural Proteins
Brefeldin A
1-Phosphatidylinositol 4-Kinase
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Sasaki, Jun ; Ishikawa, Kumiko ; Arita, Minetaro ; Taniguchi, Koki. / ACBD3-mediated recruitment of PI4KB to picornavirus RNA replication sites. In: EMBO Journal. 2012 ; Vol. 31, No. 3. pp. 754-766.
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ACBD3-mediated recruitment of PI4KB to picornavirus RNA replication sites. / Sasaki, Jun; Ishikawa, Kumiko; Arita, Minetaro; Taniguchi, Koki.

In: EMBO Journal, Vol. 31, No. 3, 01.02.2012, p. 754-766.

Research output: Contribution to journalArticle

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