Accelerated differentiation of melanocyte stem cells contributes to the formation of hyperpigmented maculae

Takaaki Yamada, Seiji Hasegawa, Yu Inoue, Yasushi Date, Masaru Arima, Akiko Yagami, Yohei Iwata, Masayuki Takahashi, Naoki Yamamoto, Hiroshi Mizutani, Satoru Nakata, Kayoko Matsunaga, Hirohiko Akamatsu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

It has been reported that the abnormal regulation of melanocyte stem cells (McSCs) causes hair greying; however, little is known about the role of McSCs in skin hyperpigmentation such as solar lentigines (SLs). To investigate the involvement of McSCs in SLs, the canonical Wnt signalling pathway that triggers the differentiation of McSCs was analysed in UVB-induced delayed hyperpigmented maculae in mice and human SL lesions. After inducing hyperpigmented maculae on dorsal skin of F1 mice of HR-1× HR/De, which was formed long after repeated UVB irradiation, the epidermal Wnt1 expression and the number of nuclear β-catenin-positive McSCs were increased as compared to non-irradiated control mice. Furthermore, the expression of dopachrome tautomerase (Dct), a downstream target of β-catenin, was significantly upregulated in McSCs of UVB-irradiated mice. The Wnt1 expression and the number of nuclear β-catenin-positive McSCs were also higher in human SL lesions than in normal skin. Recombinant Wnt1 protein induced melanocyte-related genes including Dct in early-passage normal human melanocytes (NHEMs), an in vitro McSC model. These results demonstrate that the canonical Wnt signalling pathway is activated in SL lesions and strongly suggest that the accelerated differentiation of McSCs is involved in SL pathogenesis.

Original languageEnglish
Pages (from-to)652-658
Number of pages7
JournalExperimental Dermatology
Volume23
Issue number9
DOIs
Publication statusPublished - 01-01-2014

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Melanocytes
Stem cells
Stem Cells
Lentigo
Wnt Signaling Pathway
Catenins
Skin
Wnt1 Protein
Recombinant proteins
Hyperpigmentation
Recombinant Proteins
Hair
Genes
Irradiation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Yamada, Takaaki ; Hasegawa, Seiji ; Inoue, Yu ; Date, Yasushi ; Arima, Masaru ; Yagami, Akiko ; Iwata, Yohei ; Takahashi, Masayuki ; Yamamoto, Naoki ; Mizutani, Hiroshi ; Nakata, Satoru ; Matsunaga, Kayoko ; Akamatsu, Hirohiko. / Accelerated differentiation of melanocyte stem cells contributes to the formation of hyperpigmented maculae. In: Experimental Dermatology. 2014 ; Vol. 23, No. 9. pp. 652-658.
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abstract = "It has been reported that the abnormal regulation of melanocyte stem cells (McSCs) causes hair greying; however, little is known about the role of McSCs in skin hyperpigmentation such as solar lentigines (SLs). To investigate the involvement of McSCs in SLs, the canonical Wnt signalling pathway that triggers the differentiation of McSCs was analysed in UVB-induced delayed hyperpigmented maculae in mice and human SL lesions. After inducing hyperpigmented maculae on dorsal skin of F1 mice of HR-1× HR/De, which was formed long after repeated UVB irradiation, the epidermal Wnt1 expression and the number of nuclear β-catenin-positive McSCs were increased as compared to non-irradiated control mice. Furthermore, the expression of dopachrome tautomerase (Dct), a downstream target of β-catenin, was significantly upregulated in McSCs of UVB-irradiated mice. The Wnt1 expression and the number of nuclear β-catenin-positive McSCs were also higher in human SL lesions than in normal skin. Recombinant Wnt1 protein induced melanocyte-related genes including Dct in early-passage normal human melanocytes (NHEMs), an in vitro McSC model. These results demonstrate that the canonical Wnt signalling pathway is activated in SL lesions and strongly suggest that the accelerated differentiation of McSCs is involved in SL pathogenesis.",
author = "Takaaki Yamada and Seiji Hasegawa and Yu Inoue and Yasushi Date and Masaru Arima and Akiko Yagami and Yohei Iwata and Masayuki Takahashi and Naoki Yamamoto and Hiroshi Mizutani and Satoru Nakata and Kayoko Matsunaga and Hirohiko Akamatsu",
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Yamada, T, Hasegawa, S, Inoue, Y, Date, Y, Arima, M, Yagami, A, Iwata, Y, Takahashi, M, Yamamoto, N, Mizutani, H, Nakata, S, Matsunaga, K & Akamatsu, H 2014, 'Accelerated differentiation of melanocyte stem cells contributes to the formation of hyperpigmented maculae', Experimental Dermatology, vol. 23, no. 9, pp. 652-658. https://doi.org/10.1111/exd.12496

Accelerated differentiation of melanocyte stem cells contributes to the formation of hyperpigmented maculae. / Yamada, Takaaki; Hasegawa, Seiji; Inoue, Yu; Date, Yasushi; Arima, Masaru; Yagami, Akiko; Iwata, Yohei; Takahashi, Masayuki; Yamamoto, Naoki; Mizutani, Hiroshi; Nakata, Satoru; Matsunaga, Kayoko; Akamatsu, Hirohiko.

In: Experimental Dermatology, Vol. 23, No. 9, 01.01.2014, p. 652-658.

Research output: Contribution to journalArticle

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AU - Yamada, Takaaki

AU - Hasegawa, Seiji

AU - Inoue, Yu

AU - Date, Yasushi

AU - Arima, Masaru

AU - Yagami, Akiko

AU - Iwata, Yohei

AU - Takahashi, Masayuki

AU - Yamamoto, Naoki

AU - Mizutani, Hiroshi

AU - Nakata, Satoru

AU - Matsunaga, Kayoko

AU - Akamatsu, Hirohiko

PY - 2014/1/1

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N2 - It has been reported that the abnormal regulation of melanocyte stem cells (McSCs) causes hair greying; however, little is known about the role of McSCs in skin hyperpigmentation such as solar lentigines (SLs). To investigate the involvement of McSCs in SLs, the canonical Wnt signalling pathway that triggers the differentiation of McSCs was analysed in UVB-induced delayed hyperpigmented maculae in mice and human SL lesions. After inducing hyperpigmented maculae on dorsal skin of F1 mice of HR-1× HR/De, which was formed long after repeated UVB irradiation, the epidermal Wnt1 expression and the number of nuclear β-catenin-positive McSCs were increased as compared to non-irradiated control mice. Furthermore, the expression of dopachrome tautomerase (Dct), a downstream target of β-catenin, was significantly upregulated in McSCs of UVB-irradiated mice. The Wnt1 expression and the number of nuclear β-catenin-positive McSCs were also higher in human SL lesions than in normal skin. Recombinant Wnt1 protein induced melanocyte-related genes including Dct in early-passage normal human melanocytes (NHEMs), an in vitro McSC model. These results demonstrate that the canonical Wnt signalling pathway is activated in SL lesions and strongly suggest that the accelerated differentiation of McSCs is involved in SL pathogenesis.

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