Acid hydrolase activity of cultured bovine oligodendrocytes

Mikio Hirayama; Tatsuro Mutoh; Akira Tokuda

doi:10.1023/A:1011090527561

Acid hydrolase activity of cultured bovine oligodendrocytes

Mikio Hirayama, Tatsuro Mutoh, Akira Tokuda

Research output: Contribution to journal › Article › peer-review

Abstract

We measured the activity of several acid hydrolases of cultured oligodendrocytes prepared from adult bovine brain white matter to clarify the biochemical basis of bovine oligodendrocytes in vitro. Lysosomal enzyme activities were assayed by using 4-methylumbelliferyl glycosides as substrates. Lysosomal enzyme activities became higher at 8-11 days in vitro (DIV) than 4 DIV. The enrichment in acid hydrolase specific activities in oligodendrocytes may be associated with lysosomal origin of myelin-like membranes.

Original language	English
Pages (from-to)	121-124
Number of pages	4
Journal	Neurochemical Research
Volume	26
Issue number	2
DOIs	https://doi.org/10.1023/A:1011090527561
Publication status	Published - 2001
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry
Cellular and Molecular Neuroscience

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abstract = "We measured the activity of several acid hydrolases of cultured oligodendrocytes prepared from adult bovine brain white matter to clarify the biochemical basis of bovine oligodendrocytes in vitro. Lysosomal enzyme activities were assayed by using 4-methylumbelliferyl glycosides as substrates. Lysosomal enzyme activities became higher at 8-11 days in vitro (DIV) than 4 DIV. The enrichment in acid hydrolase specific activities in oligodendrocytes may be associated with lysosomal origin of myelin-like membranes.",

author = "Mikio Hirayama and Tatsuro Mutoh and Akira Tokuda",

year = "2001",

doi = "10.1023/A:1011090527561",

language = "English",

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journal = "Neurochemical Research",

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AU - Mutoh, Tatsuro

AU - Tokuda, Akira

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AB - We measured the activity of several acid hydrolases of cultured oligodendrocytes prepared from adult bovine brain white matter to clarify the biochemical basis of bovine oligodendrocytes in vitro. Lysosomal enzyme activities were assayed by using 4-methylumbelliferyl glycosides as substrates. Lysosomal enzyme activities became higher at 8-11 days in vitro (DIV) than 4 DIV. The enrichment in acid hydrolase specific activities in oligodendrocytes may be associated with lysosomal origin of myelin-like membranes.

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Acid hydrolase activity of cultured bovine oligodendrocytes

Abstract

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