Actin nano-architecture of phagocytic podosomes

J. Cody Herron, Shiqiong Hu, Takashi Watanabe, Ana T. Nogueira, Bei Liu, Megan E. Kern, Jesse Aaron, Aaron Taylor, Michael Pablo, Teng Leong Chew, Timothy C. Elston, Klaus M. Hahn

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Podosomes are actin-enriched adhesion structures important for multiple cellular processes, including migration, bone remodeling, and phagocytosis. Here, we characterize the structure and organization of phagocytic podosomes using interferometric photoactivated localization microscopy, a super-resolution microscopy technique capable of 15–20 nm resolution, together with structured illumination microscopy and localization-based super-resolution microscopy. Phagocytic podosomes are observed during frustrated phagocytosis, a model in which cells attempt to engulf micropatterned IgG antibodies. For circular patterns, this results in regular arrays of podosomes with well-defined geometry. Using persistent homology, we develop a pipeline for semi-automatic identification and measurement of podosome features. These studies reveal an hourglass shape of the podosome actin core, a protruding knob at the bottom of the core, and two actin networks extending from the core. Additionally, the distributions of paxillin, talin, myosin II, α-actinin, cortactin, and microtubules relative to actin are characterized.

Original languageEnglish
Article number4363
JournalNature communications
Issue number1
Publication statusPublished - 12-2022

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)


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