Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling

Satoshi Yamazaki; Yo Mabuchi; Takaharu Kimura; Eriko Grace Suto; Daisuke Hisamatsu; Yuna Naraoka; Ayako Kondo; Yuzuki Azuma; Riko Kikuchi; Hidekazu Nishikii; Soji Morishita; Marito Araki; Norio Komatsu; Chihiro Akazawa

doi:10.1016/j.stemcr.2024.02.002

Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling

Satoshi Yamazaki, Yo Mabuchi, Takaharu Kimura, Eriko Grace Suto, Daisuke Hisamatsu, Yuna Naraoka, Ayako Kondo, Yuzuki Azuma, Riko Kikuchi, Hidekazu Nishikii, Soji Morishita, Marito Araki, Norio Komatsu, Chihiro Akazawa

Department of Clinical Regenerative Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Myeloid cells, which originate from hematopoietic stem/progenitor cells (HSPCs), play a crucial role in mitigating infections. This study aimed to explore the impact of mesenchymal stem/stromal cells (MSCs) on the differentiation of HSPCs and progenitors through the C-C motif chemokine CCL2/CCR2 signaling pathway. Murine MSCs, identified as PDGFRα⁺Sca-1⁺ cells (PαS cells), were found to secrete CCL2, particularly in response to lipopolysaccharide stimulation. MSC-secreted CCL2 promoted the differentiation of granulocyte/macrophage progenitors into the myeloid lineage. MSC-derived CCL2 plays an important role in the early phase of myeloid cell differentiation in vivo. Single-cell RNA sequencing analysis confirmed that CCL2-mediated cell fate determination was also observed in human bone marrow cells. These findings provide valuable insights for investigating the in vivo effects of MSC transplantation.

Original language	English
Pages (from-to)	414-425
Number of pages	12
Journal	Stem Cell Reports
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/j.stemcr.2024.02.002
Publication status	Published - 12-03-2024

All Science Journal Classification (ASJC) codes

Biochemistry
Genetics
Developmental Biology
Cell Biology

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Yamazaki, S., Mabuchi, Y., Kimura, T., Suto, E. G., Hisamatsu, D., Naraoka, Y., Kondo, A., Azuma, Y., Kikuchi, R., Nishikii, H., Morishita, S., Araki, M., Komatsu, N., & Akazawa, C. (2024). Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling. Stem Cell Reports, 19(3), 414-425. https://doi.org/10.1016/j.stemcr.2024.02.002

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title = "Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling",

abstract = "Myeloid cells, which originate from hematopoietic stem/progenitor cells (HSPCs), play a crucial role in mitigating infections. This study aimed to explore the impact of mesenchymal stem/stromal cells (MSCs) on the differentiation of HSPCs and progenitors through the C-C motif chemokine CCL2/CCR2 signaling pathway. Murine MSCs, identified as PDGFRα+Sca-1+ cells (PαS cells), were found to secrete CCL2, particularly in response to lipopolysaccharide stimulation. MSC-secreted CCL2 promoted the differentiation of granulocyte/macrophage progenitors into the myeloid lineage. MSC-derived CCL2 plays an important role in the early phase of myeloid cell differentiation in vivo. Single-cell RNA sequencing analysis confirmed that CCL2-mediated cell fate determination was also observed in human bone marrow cells. These findings provide valuable insights for investigating the in vivo effects of MSC transplantation.",

author = "Satoshi Yamazaki and Yo Mabuchi and Takaharu Kimura and Suto, {Eriko Grace} and Daisuke Hisamatsu and Yuna Naraoka and Ayako Kondo and Yuzuki Azuma and Riko Kikuchi and Hidekazu Nishikii and Soji Morishita and Marito Araki and Norio Komatsu and Chihiro Akazawa",

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year = "2024",

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doi = "10.1016/j.stemcr.2024.02.002",

language = "English",

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AU - Yamazaki, Satoshi

AU - Mabuchi, Yo

AU - Kimura, Takaharu

AU - Suto, Eriko Grace

AU - Hisamatsu, Daisuke

AU - Naraoka, Yuna

AU - Kondo, Ayako

AU - Azuma, Yuzuki

AU - Kikuchi, Riko

AU - Nishikii, Hidekazu

AU - Morishita, Soji

AU - Araki, Marito

AU - Komatsu, Norio

AU - Akazawa, Chihiro

PY - 2024/3/12

Y1 - 2024/3/12

N2 - Myeloid cells, which originate from hematopoietic stem/progenitor cells (HSPCs), play a crucial role in mitigating infections. This study aimed to explore the impact of mesenchymal stem/stromal cells (MSCs) on the differentiation of HSPCs and progenitors through the C-C motif chemokine CCL2/CCR2 signaling pathway. Murine MSCs, identified as PDGFRα+Sca-1+ cells (PαS cells), were found to secrete CCL2, particularly in response to lipopolysaccharide stimulation. MSC-secreted CCL2 promoted the differentiation of granulocyte/macrophage progenitors into the myeloid lineage. MSC-derived CCL2 plays an important role in the early phase of myeloid cell differentiation in vivo. Single-cell RNA sequencing analysis confirmed that CCL2-mediated cell fate determination was also observed in human bone marrow cells. These findings provide valuable insights for investigating the in vivo effects of MSC transplantation.

AB - Myeloid cells, which originate from hematopoietic stem/progenitor cells (HSPCs), play a crucial role in mitigating infections. This study aimed to explore the impact of mesenchymal stem/stromal cells (MSCs) on the differentiation of HSPCs and progenitors through the C-C motif chemokine CCL2/CCR2 signaling pathway. Murine MSCs, identified as PDGFRα+Sca-1+ cells (PαS cells), were found to secrete CCL2, particularly in response to lipopolysaccharide stimulation. MSC-secreted CCL2 promoted the differentiation of granulocyte/macrophage progenitors into the myeloid lineage. MSC-derived CCL2 plays an important role in the early phase of myeloid cell differentiation in vivo. Single-cell RNA sequencing analysis confirmed that CCL2-mediated cell fate determination was also observed in human bone marrow cells. These findings provide valuable insights for investigating the in vivo effects of MSC transplantation.

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Activated mesenchymal stem/stromal cells promote myeloid cell differentiation via CCL2/CCR2 signaling

Abstract

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