Activation-Induced Cytidine Deaminase Alters the Subcellular Localization of Tet Family Proteins

Yuko Arioka, Akira Watanabe, Kuniaki Saito, Yasuhiro Yamada

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Activation-induced cytidine deminase (Aid), a unique enzyme that deaminates cytosine in DNA, shuttles between the nucleus and the cytoplasm. A recent study proposed a novel function of Aid in active DNA demethylation via deamination of 5-hydroxymethylcytosine, which is converted from 5-methylcytosine by the Ten-eleven translocation (Tet) family of enzymes. In this study, we examined the effect of simultaneous expression of Aid and Tet family proteins on the subcellular localization of each protein. We found that overexpressed Aid is mainly localized in the cytoplasm, whereas Tet1 and Tet2 are localized in the nucleus, and Tet3 is localized in both the cytoplasm and the nucleus. However, nuclear Tet proteins were gradually translocated to the cytoplasm when co-expressed with Aid. We also show that Aid-mediated translocation of Tet proteins is associated with Aid shuttling. Here we propose a possible role for Aid as a regulator of the subcellular localization of Tet family proteins.

Original languageEnglish
Article numbere45031
JournalPloS one
Volume7
Issue number9
DOIs
Publication statusPublished - 17-09-2012

Fingerprint

cytidine
Cytidine
Protein Transport
Chemical activation
Cytoplasm
Proteins
cytoplasm
proteins
protein transport
5-Methylcytosine
deamination
Deamination
AICDA (activation-induced cytidine deaminase)
cytidine deaminase
cytosine
Cytosine
DNA
Enzymes
enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Arioka, Yuko ; Watanabe, Akira ; Saito, Kuniaki ; Yamada, Yasuhiro. / Activation-Induced Cytidine Deaminase Alters the Subcellular Localization of Tet Family Proteins. In: PloS one. 2012 ; Vol. 7, No. 9.
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Activation-Induced Cytidine Deaminase Alters the Subcellular Localization of Tet Family Proteins. / Arioka, Yuko; Watanabe, Akira; Saito, Kuniaki; Yamada, Yasuhiro.

In: PloS one, Vol. 7, No. 9, e45031, 17.09.2012.

Research output: Contribution to journalArticle

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T1 - Activation-Induced Cytidine Deaminase Alters the Subcellular Localization of Tet Family Proteins

AU - Arioka, Yuko

AU - Watanabe, Akira

AU - Saito, Kuniaki

AU - Yamada, Yasuhiro

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