TY - JOUR
T1 - Activation of a Nodal-independent signaling pathway by Cripto-1 mutants with impaired activation of a Nodal-dependent signaling pathway
AU - Bianco, Caterina
AU - Mysliwiec, Margaret
AU - Watanabe, Kazuhide
AU - Mancino, Mario
AU - Nagaoka, Tadahiro
AU - Gonzales, Monica
AU - Salomon, David S.
N1 - Funding Information:
We would like to thank Pamela Stanley (Albert Einstein College of Medicine, New York) for generously providing the Cripto-1 mutant plasmids. We would like to thank Christina Baraty for her excellent technical assistance. This work was supported by Intramural Research program of the NIH, NCI, CCR.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2008/12/10
Y1 - 2008/12/10
N2 - Cripto-1, a co-receptor for Nodal, can activate Nodal-dependent and Nodal-independent signaling pathways. In this study we have investigated whether Cripto-1 mutants, that fail to activate a Nodal-dependent signaling pathway, are capable to activate a Nodal-independent signaling pathway in mammary epithelial cells. Cripto-1 mutants expressed in EpH4 mouse mammary epithelial cells are fully functional in regard to activation of a Nodal-independent signaling pathway, leading to phosphorylation of mitogen-activated protein kinase (MAPK) and Akt and to enhanced proliferation and motility of these cells, suggesting that Cripto-1 mutants with impaired Nodal signaling are still active in a Nodal-independent signaling pathway. Structured summary: MINT-6797299:Glypican 1 (uniprotkb:P35052) physically interacts (MI:0218) with Cr 1 (uniprotkb:P13385) by anti bait coimmunoprecipitation (MI:0006).
AB - Cripto-1, a co-receptor for Nodal, can activate Nodal-dependent and Nodal-independent signaling pathways. In this study we have investigated whether Cripto-1 mutants, that fail to activate a Nodal-dependent signaling pathway, are capable to activate a Nodal-independent signaling pathway in mammary epithelial cells. Cripto-1 mutants expressed in EpH4 mouse mammary epithelial cells are fully functional in regard to activation of a Nodal-independent signaling pathway, leading to phosphorylation of mitogen-activated protein kinase (MAPK) and Akt and to enhanced proliferation and motility of these cells, suggesting that Cripto-1 mutants with impaired Nodal signaling are still active in a Nodal-independent signaling pathway. Structured summary: MINT-6797299:Glypican 1 (uniprotkb:P35052) physically interacts (MI:0218) with Cr 1 (uniprotkb:P13385) by anti bait coimmunoprecipitation (MI:0006).
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U2 - 10.1016/j.febslet.2008.10.052
DO - 10.1016/j.febslet.2008.10.052
M3 - Article
C2 - 19013461
AN - SCOPUS:56949098504
VL - 582
SP - 3997
EP - 4002
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 29
ER -