Activation of a Nodal-independent signaling pathway by Cripto-1 mutants with impaired activation of a Nodal-dependent signaling pathway

Caterina Bianco; Margaret Mysliwiec; Kazuhide Watanabe; Mario Mancino; Tadahiro Nagaoka; Monica Gonzales; David S. Salomon

doi:10.1016/j.febslet.2008.10.052

Activation of a Nodal-independent signaling pathway by Cripto-1 mutants with impaired activation of a Nodal-dependent signaling pathway

Caterina Bianco, Margaret Mysliwiec, Kazuhide Watanabe, Mario Mancino, Tadahiro Nagaoka, Monica Gonzales, David S. Salomon

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Cripto-1, a co-receptor for Nodal, can activate Nodal-dependent and Nodal-independent signaling pathways. In this study we have investigated whether Cripto-1 mutants, that fail to activate a Nodal-dependent signaling pathway, are capable to activate a Nodal-independent signaling pathway in mammary epithelial cells. Cripto-1 mutants expressed in EpH4 mouse mammary epithelial cells are fully functional in regard to activation of a Nodal-independent signaling pathway, leading to phosphorylation of mitogen-activated protein kinase (MAPK) and Akt and to enhanced proliferation and motility of these cells, suggesting that Cripto-1 mutants with impaired Nodal signaling are still active in a Nodal-independent signaling pathway. Structured summary: MINT-6797299:Glypican 1 (uniprotkb:P35052) physically interacts (MI:0218) with Cr 1 (uniprotkb:P13385) by anti bait coimmunoprecipitation (MI:0006).

Original language	English
Pages (from-to)	3997-4002
Number of pages	6
Journal	FEBS Letters
Volume	582
Issue number	29
DOIs	https://doi.org/10.1016/j.febslet.2008.10.052
Publication status	Published - 10-12-2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2008.10.052

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@article{3b24b3465c0b4ca8a1fd4be5254cdbfd,

title = "Activation of a Nodal-independent signaling pathway by Cripto-1 mutants with impaired activation of a Nodal-dependent signaling pathway",

abstract = "Cripto-1, a co-receptor for Nodal, can activate Nodal-dependent and Nodal-independent signaling pathways. In this study we have investigated whether Cripto-1 mutants, that fail to activate a Nodal-dependent signaling pathway, are capable to activate a Nodal-independent signaling pathway in mammary epithelial cells. Cripto-1 mutants expressed in EpH4 mouse mammary epithelial cells are fully functional in regard to activation of a Nodal-independent signaling pathway, leading to phosphorylation of mitogen-activated protein kinase (MAPK) and Akt and to enhanced proliferation and motility of these cells, suggesting that Cripto-1 mutants with impaired Nodal signaling are still active in a Nodal-independent signaling pathway. Structured summary: MINT-6797299:Glypican 1 (uniprotkb:P35052) physically interacts (MI:0218) with Cr 1 (uniprotkb:P13385) by anti bait coimmunoprecipitation (MI:0006).",

author = "Caterina Bianco and Margaret Mysliwiec and Kazuhide Watanabe and Mario Mancino and Tadahiro Nagaoka and Monica Gonzales and Salomon, {David S.}",

note = "Funding Information: We would like to thank Pamela Stanley (Albert Einstein College of Medicine, New York) for generously providing the Cripto-1 mutant plasmids. We would like to thank Christina Baraty for her excellent technical assistance. This work was supported by Intramural Research program of the NIH, NCI, CCR.",

year = "2008",

month = dec,

day = "10",

doi = "10.1016/j.febslet.2008.10.052",

language = "English",

volume = "582",

pages = "3997--4002",

journal = "FEBS Letters",

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TY - JOUR

T1 - Activation of a Nodal-independent signaling pathway by Cripto-1 mutants with impaired activation of a Nodal-dependent signaling pathway

AU - Bianco, Caterina

AU - Mysliwiec, Margaret

AU - Watanabe, Kazuhide

AU - Mancino, Mario

AU - Nagaoka, Tadahiro

AU - Gonzales, Monica

AU - Salomon, David S.

N1 - Funding Information: We would like to thank Pamela Stanley (Albert Einstein College of Medicine, New York) for generously providing the Cripto-1 mutant plasmids. We would like to thank Christina Baraty for her excellent technical assistance. This work was supported by Intramural Research program of the NIH, NCI, CCR.

PY - 2008/12/10

Y1 - 2008/12/10

N2 - Cripto-1, a co-receptor for Nodal, can activate Nodal-dependent and Nodal-independent signaling pathways. In this study we have investigated whether Cripto-1 mutants, that fail to activate a Nodal-dependent signaling pathway, are capable to activate a Nodal-independent signaling pathway in mammary epithelial cells. Cripto-1 mutants expressed in EpH4 mouse mammary epithelial cells are fully functional in regard to activation of a Nodal-independent signaling pathway, leading to phosphorylation of mitogen-activated protein kinase (MAPK) and Akt and to enhanced proliferation and motility of these cells, suggesting that Cripto-1 mutants with impaired Nodal signaling are still active in a Nodal-independent signaling pathway. Structured summary: MINT-6797299:Glypican 1 (uniprotkb:P35052) physically interacts (MI:0218) with Cr 1 (uniprotkb:P13385) by anti bait coimmunoprecipitation (MI:0006).

AB - Cripto-1, a co-receptor for Nodal, can activate Nodal-dependent and Nodal-independent signaling pathways. In this study we have investigated whether Cripto-1 mutants, that fail to activate a Nodal-dependent signaling pathway, are capable to activate a Nodal-independent signaling pathway in mammary epithelial cells. Cripto-1 mutants expressed in EpH4 mouse mammary epithelial cells are fully functional in regard to activation of a Nodal-independent signaling pathway, leading to phosphorylation of mitogen-activated protein kinase (MAPK) and Akt and to enhanced proliferation and motility of these cells, suggesting that Cripto-1 mutants with impaired Nodal signaling are still active in a Nodal-independent signaling pathway. Structured summary: MINT-6797299:Glypican 1 (uniprotkb:P35052) physically interacts (MI:0218) with Cr 1 (uniprotkb:P13385) by anti bait coimmunoprecipitation (MI:0006).

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Activation of a Nodal-independent signaling pathway by Cripto-1 mutants with impaired activation of a Nodal-dependent signaling pathway

Abstract

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