Activation of a novel human transforming gene, ret, by DNA rearrangement

Masahide Takahashi; Jerome Ritz; Geoffrey M. Cooper

doi:10.1016/0092-8674(85)90115-1

Activation of a novel human transforming gene, ret, by DNA rearrangement

Masahide Takahashi, Jerome Ritz, Geoffrey M. Cooper

Research output: Contribution to journal › Article › peer-review

711 Citations (Scopus)

Abstract

A novel transforming gene was detected by transfection of NIH 3T3 cells with human lymphoma DNA. The tumor DNA induced a single focus in primary transfections, whereas DNAs of transformed NIH cells induced transformation with high efficiencies in secondary and tertiary assays. Molecular clones spanning about 37 kb of human sequence were isolated from tertiary transformant DNA. Blot hybridization indicated that the transforming gene consisted of two segments that were unlinked in both normal human and primary lymphoma DNAs. The two segments of human DNA were cotranscribed in transformed NIH cells but not in any human cells examined. The transforming gene thus appeared to be activated by recombination between two unlinked human DNA segments, possibly by cointegration during transfection.

Original language	English
Pages (from-to)	581-588
Number of pages	8
Journal	Cell
Volume	42
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(85)90115-1
Publication status	Published - 09-1985
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology

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10.1016/0092-8674(85)90115-1

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title = "Activation of a novel human transforming gene, ret, by DNA rearrangement",

abstract = "A novel transforming gene was detected by transfection of NIH 3T3 cells with human lymphoma DNA. The tumor DNA induced a single focus in primary transfections, whereas DNAs of transformed NIH cells induced transformation with high efficiencies in secondary and tertiary assays. Molecular clones spanning about 37 kb of human sequence were isolated from tertiary transformant DNA. Blot hybridization indicated that the transforming gene consisted of two segments that were unlinked in both normal human and primary lymphoma DNAs. The two segments of human DNA were cotranscribed in transformed NIH cells but not in any human cells examined. The transforming gene thus appeared to be activated by recombination between two unlinked human DNA segments, possibly by cointegration during transfection.",

author = "Masahide Takahashi and Jerome Ritz and Cooper, {Geoffrey M.}",

note = "Funding Information: We are grateful to C. Der and A. Diamond for helpful comments on the manuscript and to colleagues who provided oncogene molecular clones. This research was supported by grants from the National Cancer Institute, by a Leukemia Society of America Scholarship to J. R., and by an American Cancer Society Faculty Research Award to G. M. C.",

year = "1985",

month = sep,

doi = "10.1016/0092-8674(85)90115-1",

language = "English",

volume = "42",

pages = "581--588",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

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T1 - Activation of a novel human transforming gene, ret, by DNA rearrangement

AU - Takahashi, Masahide

AU - Ritz, Jerome

AU - Cooper, Geoffrey M.

N1 - Funding Information: We are grateful to C. Der and A. Diamond for helpful comments on the manuscript and to colleagues who provided oncogene molecular clones. This research was supported by grants from the National Cancer Institute, by a Leukemia Society of America Scholarship to J. R., and by an American Cancer Society Faculty Research Award to G. M. C.

PY - 1985/9

Y1 - 1985/9

N2 - A novel transforming gene was detected by transfection of NIH 3T3 cells with human lymphoma DNA. The tumor DNA induced a single focus in primary transfections, whereas DNAs of transformed NIH cells induced transformation with high efficiencies in secondary and tertiary assays. Molecular clones spanning about 37 kb of human sequence were isolated from tertiary transformant DNA. Blot hybridization indicated that the transforming gene consisted of two segments that were unlinked in both normal human and primary lymphoma DNAs. The two segments of human DNA were cotranscribed in transformed NIH cells but not in any human cells examined. The transforming gene thus appeared to be activated by recombination between two unlinked human DNA segments, possibly by cointegration during transfection.

AB - A novel transforming gene was detected by transfection of NIH 3T3 cells with human lymphoma DNA. The tumor DNA induced a single focus in primary transfections, whereas DNAs of transformed NIH cells induced transformation with high efficiencies in secondary and tertiary assays. Molecular clones spanning about 37 kb of human sequence were isolated from tertiary transformant DNA. Blot hybridization indicated that the transforming gene consisted of two segments that were unlinked in both normal human and primary lymphoma DNAs. The two segments of human DNA were cotranscribed in transformed NIH cells but not in any human cells examined. The transforming gene thus appeared to be activated by recombination between two unlinked human DNA segments, possibly by cointegration during transfection.

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Activation of a novel human transforming gene, ret, by DNA rearrangement

Abstract

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