TY - JOUR
T1 - Activation of protein kinase C by non-phorbol tumor promoter, mezerein
AU - Miyake, Ryohei
AU - Tanaka, Yasushi
AU - Tsuda, Terutaka
AU - Kaibuchi, Kozo
AU - Kikkawa, Ushio
AU - Nishizuka, Yasutomi
N1 - Funding Information:
*/ This investigation was supported in part by research grants from the SciTntific Research Fund of the Ministry of Education, Science and Culture, Japan (1982-1984), the Intractable Diseases Division, Public Health Bureau, the Ministry of Health and Welfare, Japan (1982-1984), a Grant-in-Aid of New Drug Development from the Ministry of Health and Welfare, Japan (1983), the Science and Technology Agency (1983), the Yamanouchi Foundation for Research on Metabolic Disorders (1982-1983), and Mitsuhisa Memorial Cancer Research Fund (1983). The data are taken in part from the dissertation that will be submitted by R. Miyake to Kobe University School of Medicine in partial fulfilment of the requirement for the degree of Doctor of Medical Science. l/ The-abbreviations used are: TPA, 12-O-tetradecanoylphorbol-13-acetate; PDBF, phorbol-12,13-dibutyrate; EGTA, ethylene glycol bis(@aminoethyl ether)-N,N,N',N'-tetraacetic acid; DMSO, dimethyl sulfoxide.
PY - 1984/6/15
Y1 - 1984/6/15
N2 - Mezerein, classified as a second-stage tumor promoter, has no diacylglycerol-like structure in its molecule, but can activate protein kinase C both in vitro and in vivo. This non-phorbol diterpene competitively inhibits the specific binding of a radioactive tumor-promoting phorbol ester to the enzyme. It is suggestive that tumor-promoting phorbol esters and mezerein cause analogous changes in the membrane to activate protein kinase C, and utilize this protein kinase as a common receptive protein for tumor promotion.
AB - Mezerein, classified as a second-stage tumor promoter, has no diacylglycerol-like structure in its molecule, but can activate protein kinase C both in vitro and in vivo. This non-phorbol diterpene competitively inhibits the specific binding of a radioactive tumor-promoting phorbol ester to the enzyme. It is suggestive that tumor-promoting phorbol esters and mezerein cause analogous changes in the membrane to activate protein kinase C, and utilize this protein kinase as a common receptive protein for tumor promotion.
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U2 - 10.1016/0006-291X(84)90231-6
DO - 10.1016/0006-291X(84)90231-6
M3 - Article
C2 - 6233978
AN - SCOPUS:0021281898
VL - 121
SP - 649
EP - 656
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -