Activin-like signaling activates Notch signaling during mesodermal induction

Takanori Abe, Miho Furue, Yasufumi Myoish, Tetsuji Okamoto, Akiko Kondo, Makoto Asashima

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Both activin-like signaling and Notch signaling play fundamental roles during early development. Activin-like signaling is involved in mesodermal induction and can induce a broad range of mesodermal genes and tissues from prospective ectodermal cells (animal caps). On the other hand, Notch signaling plays important roles when multipotent precursor cells achieve a specific cell fate. However, the relationship between these two signal pathways is not well understood. Here, we show that activin A induces Delta-1, Delta-2 and Notch expression and then activates Notch signaling in animal caps. Also, in vivo, ectopic activin-like signaling induced the ectopic expression of Delta-1 and Delta-2, whereas inhibition of activin-like signaling abolished the expression of Delta-1 and Delta-2. Furthermore, we show that MyoD, which is myogenic gene induced by activin A, can induce Delta-1 expression. However, MyoD had no effect on Notch expression, and inhibited Delta-2 expression. These results indicated that activin A induces Delta-1, Delta-2 and Notch by different cascades. We conclude that Notch signaling is activated when activin-like signaling induces various tissues from homogenous undifferentiated cells.

Original languageEnglish
Pages (from-to)327-332
Number of pages6
JournalInternational Journal of Developmental Biology
Volume48
Issue number4
DOIs
Publication statusPublished - 01-06-2004

Fingerprint

Activins
Genes
Signal Transduction
activin A

All Science Journal Classification (ASJC) codes

  • Embryology
  • Developmental Biology

Cite this

Abe, Takanori ; Furue, Miho ; Myoish, Yasufumi ; Okamoto, Tetsuji ; Kondo, Akiko ; Asashima, Makoto. / Activin-like signaling activates Notch signaling during mesodermal induction. In: International Journal of Developmental Biology. 2004 ; Vol. 48, No. 4. pp. 327-332.
@article{60e6cc7b03a04af9bb6723b2ad888a16,
title = "Activin-like signaling activates Notch signaling during mesodermal induction",
abstract = "Both activin-like signaling and Notch signaling play fundamental roles during early development. Activin-like signaling is involved in mesodermal induction and can induce a broad range of mesodermal genes and tissues from prospective ectodermal cells (animal caps). On the other hand, Notch signaling plays important roles when multipotent precursor cells achieve a specific cell fate. However, the relationship between these two signal pathways is not well understood. Here, we show that activin A induces Delta-1, Delta-2 and Notch expression and then activates Notch signaling in animal caps. Also, in vivo, ectopic activin-like signaling induced the ectopic expression of Delta-1 and Delta-2, whereas inhibition of activin-like signaling abolished the expression of Delta-1 and Delta-2. Furthermore, we show that MyoD, which is myogenic gene induced by activin A, can induce Delta-1 expression. However, MyoD had no effect on Notch expression, and inhibited Delta-2 expression. These results indicated that activin A induces Delta-1, Delta-2 and Notch by different cascades. We conclude that Notch signaling is activated when activin-like signaling induces various tissues from homogenous undifferentiated cells.",
author = "Takanori Abe and Miho Furue and Yasufumi Myoish and Tetsuji Okamoto and Akiko Kondo and Makoto Asashima",
year = "2004",
month = "6",
day = "1",
doi = "10.1387/ijdb.041838ta",
language = "English",
volume = "48",
pages = "327--332",
journal = "International Journal of Developmental Biology",
issn = "0214-6282",
publisher = "University of the Basque Country Press",
number = "4",

}

Activin-like signaling activates Notch signaling during mesodermal induction. / Abe, Takanori; Furue, Miho; Myoish, Yasufumi; Okamoto, Tetsuji; Kondo, Akiko; Asashima, Makoto.

In: International Journal of Developmental Biology, Vol. 48, No. 4, 01.06.2004, p. 327-332.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Activin-like signaling activates Notch signaling during mesodermal induction

AU - Abe, Takanori

AU - Furue, Miho

AU - Myoish, Yasufumi

AU - Okamoto, Tetsuji

AU - Kondo, Akiko

AU - Asashima, Makoto

PY - 2004/6/1

Y1 - 2004/6/1

N2 - Both activin-like signaling and Notch signaling play fundamental roles during early development. Activin-like signaling is involved in mesodermal induction and can induce a broad range of mesodermal genes and tissues from prospective ectodermal cells (animal caps). On the other hand, Notch signaling plays important roles when multipotent precursor cells achieve a specific cell fate. However, the relationship between these two signal pathways is not well understood. Here, we show that activin A induces Delta-1, Delta-2 and Notch expression and then activates Notch signaling in animal caps. Also, in vivo, ectopic activin-like signaling induced the ectopic expression of Delta-1 and Delta-2, whereas inhibition of activin-like signaling abolished the expression of Delta-1 and Delta-2. Furthermore, we show that MyoD, which is myogenic gene induced by activin A, can induce Delta-1 expression. However, MyoD had no effect on Notch expression, and inhibited Delta-2 expression. These results indicated that activin A induces Delta-1, Delta-2 and Notch by different cascades. We conclude that Notch signaling is activated when activin-like signaling induces various tissues from homogenous undifferentiated cells.

AB - Both activin-like signaling and Notch signaling play fundamental roles during early development. Activin-like signaling is involved in mesodermal induction and can induce a broad range of mesodermal genes and tissues from prospective ectodermal cells (animal caps). On the other hand, Notch signaling plays important roles when multipotent precursor cells achieve a specific cell fate. However, the relationship between these two signal pathways is not well understood. Here, we show that activin A induces Delta-1, Delta-2 and Notch expression and then activates Notch signaling in animal caps. Also, in vivo, ectopic activin-like signaling induced the ectopic expression of Delta-1 and Delta-2, whereas inhibition of activin-like signaling abolished the expression of Delta-1 and Delta-2. Furthermore, we show that MyoD, which is myogenic gene induced by activin A, can induce Delta-1 expression. However, MyoD had no effect on Notch expression, and inhibited Delta-2 expression. These results indicated that activin A induces Delta-1, Delta-2 and Notch by different cascades. We conclude that Notch signaling is activated when activin-like signaling induces various tissues from homogenous undifferentiated cells.

UR - http://www.scopus.com/inward/record.url?scp=3142703146&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3142703146&partnerID=8YFLogxK

U2 - 10.1387/ijdb.041838ta

DO - 10.1387/ijdb.041838ta

M3 - Article

VL - 48

SP - 327

EP - 332

JO - International Journal of Developmental Biology

JF - International Journal of Developmental Biology

SN - 0214-6282

IS - 4

ER -