TY - JOUR
T1 - Adenovirus-mediated gene transfer of B7.1 induces immunological anti-tumor effects in a murine brain tumor
AU - Morioka, Jun
AU - Kajiwara, Koji
AU - Yoshikawa, Koichi
AU - Ideguchi, Makoto
AU - Uchida, Tetsuya
AU - Ohmoto, Yoshinori
AU - Suzuki, Michiyasu
N1 - Funding Information:
We thank Drs. Andrew P. Byrnes, Harry M. Charlton and Matthew J.A. Wood for the generous gift of adenovirus vectors, Professor Kathryn J. Wood for providing antibodies. This work was funded by a Research Grant from the Ministry of Education, Science and Culture of Japan.
PY - 2002/10/1
Y1 - 2002/10/1
N2 - The purpose of the present study was to determine if adenovirus-mediated transfection of a syngeneic mouse brain tumor with the gene encoding B7.1 enhances immunogenicity against tumor. Malignant astrocytoma cells were transfected with adenoviral vectors carrying the B7.1 gene (AdB7). Immunocytochemical analysis confirmed the expression of B7.1 in vitro and in vivo. To investigate the effects of B7.1 expression on tumorigenicity of the malignant astrocytoma, mice were implanted intracerebrally with B7.1-transfected glioma cells. There was no significant difference in proliferation between B7.1-transfected cells and controls in vitro. Nevertheless, mice implanted with B7.1-transfected cells survived significantly longer than those in the control groups. Immunocytochemical analysis of the tumors showed that there was infiltration of a number of CD8+ T-cells and CD25+ activated T-cells in the brain implanted with B7.1-transfected glioma cells. The results showed the possibility that adenovirus-mediated B7.1 gene transfection to a brain tumor induced activation of CD8+ cytotoxic T-lymphocytes.
AB - The purpose of the present study was to determine if adenovirus-mediated transfection of a syngeneic mouse brain tumor with the gene encoding B7.1 enhances immunogenicity against tumor. Malignant astrocytoma cells were transfected with adenoviral vectors carrying the B7.1 gene (AdB7). Immunocytochemical analysis confirmed the expression of B7.1 in vitro and in vivo. To investigate the effects of B7.1 expression on tumorigenicity of the malignant astrocytoma, mice were implanted intracerebrally with B7.1-transfected glioma cells. There was no significant difference in proliferation between B7.1-transfected cells and controls in vitro. Nevertheless, mice implanted with B7.1-transfected cells survived significantly longer than those in the control groups. Immunocytochemical analysis of the tumors showed that there was infiltration of a number of CD8+ T-cells and CD25+ activated T-cells in the brain implanted with B7.1-transfected glioma cells. The results showed the possibility that adenovirus-mediated B7.1 gene transfection to a brain tumor induced activation of CD8+ cytotoxic T-lymphocytes.
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U2 - 10.1023/A:1020260822669
DO - 10.1023/A:1020260822669
M3 - Article
C2 - 12416541
AN - SCOPUS:0036794249
SN - 0167-594X
VL - 60
SP - 13
EP - 23
JO - Journal of Neuro-Oncology
JF - Journal of Neuro-Oncology
IS - 1
ER -
