Adhesion preventive effect of hyaluronic acid after intraperitoneal surgery in mice

Tomio Sawada, Kiyoshi Hasegawa, Kazuhiko Tsukada, Seiji Kawakami

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28 Citations (Scopus)


Prevention of intraperitoneal adhesion after gynaecological surgery is essential for maintaining postoperative fertility. In this study, the adhesion prevention effect was examined of a hyaluronic acid (HA) solution obtained from the fermentation method and having a molecular weight of 1.9 x 106 with high viscosity. Laparotomy was conducted on female mice 7 weeks old, whose menstrual periods were synchronized by pregnant mare serum gonadotrophin (PMSG) to injure the uterine horn surface. Intraperitoneal adhesions were favourably formed in 91.7% of cases induced with iodine abrasion, compared with 50% induced by electrosurgery. Intraperitoneal administration of HA was evaluated for its effect on the prevention of adhesions made by iodine abrasion. Adhesion prevention effects of HA were observed at concentrations of 0.3, 0.5, 0.75 and 1.0%, among which the most pronounced effect was with the use of a 0.3% solution (92.3% of cases). Compared with the control group adhesion score of 2.0 ± 0.8, significant decreases in adhesion scores were observed at all concentrations. HA with a molecular weight of 1.9 x 106 was recognized to have a definitive prevention effect on postoperative adhesions in mice after laparotomy and is considered to be a prospective material for future clinical use.

Original languageEnglish
Pages (from-to)1470-1472
Number of pages3
JournalHuman Reproduction
Issue number6
Publication statusPublished - 1999

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology


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