Adhesion via CD43 induces Syk activation and cell proliferation in TF-1 cells

Yasuo Miura, Chisato Mizutani, Toshio Nishihara, Terutoshi Hishita, Shigeru Yanagi, Yumi Tohyama, Satoshi Ichiyama, Hirohei Yamamura, Takashi Uchiyama, Kaoru Tohyama

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10 Citations (Scopus)

Abstract

The effect of adhesion via CD43 (leukosialin, sialophorin) on cell proliferation and phosphorylation signaling were examined in a growth factor-dependent hematopoietic progenitor cell line, TF-1. TF-1 cells promptly resulted in death after withdrawal of growth factors. However, the viable cell number increased when TF-1 cells were cultured on anti-CD43 monoclonal antibody-coated plates. In this case, sustained activation of protein tyrosine kinase Syk and extracellular signal-regulated kinase (Erk) 1/2 were detected. Overexpression of exogenous Syk on TF-1 cells by the adenovirus vector system induced enhancement of the cell proliferation accompanied with enhancement of the Erk activation by a dominant-positive effect. The signal transducer and activator of transcription (STAT) 5 seemed not to be associated with the CD43-mediated cell proliferation. These results indicated that adhesion via CD43 induces the proliferation of TF-1 cells in the absence of growth factors in part by Syk-dependent Erk 1/2 signaling.

Original languageEnglish
Pages (from-to)80-86
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume288
Issue number1
DOIs
Publication statusPublished - 19-10-2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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