Adipsin-dependent secretion of hepatocyte growth factor regulates the adipocyte-cancer stem cell interaction

Masahiro Mizuno; Behnoush Khaledian; Masao Maeda; Takanori Hayashi; Seiya Mizuno; Eiji Munetsuna; Takashi Watanabe; Seishi Kono; Seiji Okada; Motoshi Suzuki; Shintaro Takao; Hironobu Minami; Naoya Asai; Fumihiro Sugiyama; Satoru Takahashi; Yohei Shimono

doi:10.3390/cancers13164238

Adipsin-dependent secretion of hepatocyte growth factor regulates the adipocyte-cancer stem cell interaction

Masahiro Mizuno, Behnoush Khaledian, Masao Maeda, Takanori Hayashi, Seiya Mizuno, Eiji Munetsuna, Takashi Watanabe, Seishi Kono, Seiji Okada, Motoshi Suzuki, Shintaro Takao, Hironobu Minami, Naoya Asai, Fumihiro Sugiyama, Satoru Takahashi, Yohei Shimono

visiting faculty

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Adipose tissue is a component of the tumor microenvironment and is involved in tumor progression. We have previously shown that adipokine adipsin (CFD) functions as an enhancer of tumor proliferation and cancer stem cell (CSC) properties in breast cancers. We established the Cfd-knockout (KO) mice and the mammary adipose tissue-derived stem cells (mADSCs) from them. Cfd-KO in mADSCs significantly reduced their ability to enhance tumorsphere formation of breast cancer patient-derived xenograft (PDX) cells, which was restored by the addition of Cfd in the culture medium. Hepatocyte growth factor (HGF) was expressed and secreted from mADSCs in a Cfd-dependent manner. HGF rescued the reduced ability of Cfd-KO mADSCs to promote tumorsphere formation in vitro and tumor formation in vivo by breast cancer PDX cells. These results suggest that HGF is a downstream effector of Cfd in mADSCs that enhances the CSC properties in breast cancers.

Original language	English
Article number	4238
Journal	Cancers
Volume	13
Issue number	16
DOIs	https://doi.org/10.3390/cancers13164238
Publication status	Published - 02-08-2021

All Science Journal Classification (ASJC) codes

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/cancers13164238

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Mizuno, M., Khaledian, B., Maeda, M., Hayashi, T., Mizuno, S., Munetsuna, E., Watanabe, T., Kono, S., Okada, S., Suzuki, M., Takao, S., Minami, H., Asai, N., Sugiyama, F., Takahashi, S., & Shimono, Y. (2021). Adipsin-dependent secretion of hepatocyte growth factor regulates the adipocyte-cancer stem cell interaction. Cancers, 13(16), Article 4238. https://doi.org/10.3390/cancers13164238

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title = "Adipsin-dependent secretion of hepatocyte growth factor regulates the adipocyte-cancer stem cell interaction",

abstract = "Adipose tissue is a component of the tumor microenvironment and is involved in tumor progression. We have previously shown that adipokine adipsin (CFD) functions as an enhancer of tumor proliferation and cancer stem cell (CSC) properties in breast cancers. We established the Cfd-knockout (KO) mice and the mammary adipose tissue-derived stem cells (mADSCs) from them. Cfd-KO in mADSCs significantly reduced their ability to enhance tumorsphere formation of breast cancer patient-derived xenograft (PDX) cells, which was restored by the addition of Cfd in the culture medium. Hepatocyte growth factor (HGF) was expressed and secreted from mADSCs in a Cfd-dependent manner. HGF rescued the reduced ability of Cfd-KO mADSCs to promote tumorsphere formation in vitro and tumor formation in vivo by breast cancer PDX cells. These results suggest that HGF is a downstream effector of Cfd in mADSCs that enhances the CSC properties in breast cancers.",

keywords = "Adipocyte, Adipsin, Breast cancer, Cancer stem cells, Hepatocyte growth factor",

author = "Masahiro Mizuno and Behnoush Khaledian and Masao Maeda and Takanori Hayashi and Seiya Mizuno and Eiji Munetsuna and Takashi Watanabe and Seishi Kono and Seiji Okada and Motoshi Suzuki and Shintaro Takao and Hironobu Minami and Naoya Asai and Fumihiro Sugiyama and Satoru Takahashi and Yohei Shimono",

note = "Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = aug,

day = "2",

doi = "10.3390/cancers13164238",

language = "English",

volume = "13",

journal = "Cancers",

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Mizuno, M, Khaledian, B, Maeda, M, Hayashi, T, Mizuno, S, Munetsuna, E, Watanabe, T, Kono, S, Okada, S, Suzuki, M, Takao, S, Minami, H, Asai, N, Sugiyama, F, Takahashi, S & Shimono, Y 2021, 'Adipsin-dependent secretion of hepatocyte growth factor regulates the adipocyte-cancer stem cell interaction', Cancers, vol. 13, no. 16, 4238. https://doi.org/10.3390/cancers13164238

TY - JOUR

T1 - Adipsin-dependent secretion of hepatocyte growth factor regulates the adipocyte-cancer stem cell interaction

AU - Mizuno, Masahiro

AU - Khaledian, Behnoush

AU - Maeda, Masao

AU - Hayashi, Takanori

AU - Mizuno, Seiya

AU - Munetsuna, Eiji

AU - Watanabe, Takashi

AU - Kono, Seishi

AU - Okada, Seiji

AU - Suzuki, Motoshi

AU - Takao, Shintaro

AU - Minami, Hironobu

AU - Asai, Naoya

AU - Sugiyama, Fumihiro

AU - Takahashi, Satoru

AU - Shimono, Yohei

PY - 2021/8/2

Y1 - 2021/8/2

N2 - Adipose tissue is a component of the tumor microenvironment and is involved in tumor progression. We have previously shown that adipokine adipsin (CFD) functions as an enhancer of tumor proliferation and cancer stem cell (CSC) properties in breast cancers. We established the Cfd-knockout (KO) mice and the mammary adipose tissue-derived stem cells (mADSCs) from them. Cfd-KO in mADSCs significantly reduced their ability to enhance tumorsphere formation of breast cancer patient-derived xenograft (PDX) cells, which was restored by the addition of Cfd in the culture medium. Hepatocyte growth factor (HGF) was expressed and secreted from mADSCs in a Cfd-dependent manner. HGF rescued the reduced ability of Cfd-KO mADSCs to promote tumorsphere formation in vitro and tumor formation in vivo by breast cancer PDX cells. These results suggest that HGF is a downstream effector of Cfd in mADSCs that enhances the CSC properties in breast cancers.

AB - Adipose tissue is a component of the tumor microenvironment and is involved in tumor progression. We have previously shown that adipokine adipsin (CFD) functions as an enhancer of tumor proliferation and cancer stem cell (CSC) properties in breast cancers. We established the Cfd-knockout (KO) mice and the mammary adipose tissue-derived stem cells (mADSCs) from them. Cfd-KO in mADSCs significantly reduced their ability to enhance tumorsphere formation of breast cancer patient-derived xenograft (PDX) cells, which was restored by the addition of Cfd in the culture medium. Hepatocyte growth factor (HGF) was expressed and secreted from mADSCs in a Cfd-dependent manner. HGF rescued the reduced ability of Cfd-KO mADSCs to promote tumorsphere formation in vitro and tumor formation in vivo by breast cancer PDX cells. These results suggest that HGF is a downstream effector of Cfd in mADSCs that enhances the CSC properties in breast cancers.

KW - Adipocyte

KW - Adipsin

KW - Breast cancer

KW - Cancer stem cells

KW - Hepatocyte growth factor

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UR - http://www.scopus.com/inward/citedby.url?scp=85113791978&partnerID=8YFLogxK

U2 - 10.3390/cancers13164238

DO - 10.3390/cancers13164238

M3 - Article

AN - SCOPUS:85113791978

SN - 2072-6694

VL - 13

JO - Cancers

JF - Cancers

IS - 16

M1 - 4238

ER -

Adipsin-dependent secretion of hepatocyte growth factor regulates the adipocyte-cancer stem cell interaction

Abstract

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