Adoptive transfer of genetically engineered WT1-specific cytotoxic T lymphocytes does not induce renal injury

Hiroaki Asai, Hiroshi Fujiwara, Sohei Kitazawa, Naoto Kobayashi, Toshiki Ochi, Yukihiro Miyazaki, Fumihiro Ochi, Yoshiki Akatsuka, Sachiko Okamoto, Junichi Mineno, Kiyotaka Kuzushima, Hiroaki Ikeda, Hiroshi Shiku, Masaki Yasukawa

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Because WT1 is expressed in leukemia cells, the development of cancer immunotherapy targeting WT1 has been an attractive translational research topic. However, concern of this therapy still remains, since WT1 is abundantly expressed in renal glomerular podocytes. In the present study, we clearly showed that WT1-specific cytotoxic T lymphocytes (CTLs) certainly exerted cytotoxicity against podocytes in vitro; however, they did not damage podocytes in vivo. This might be due to the anatomical localization of podocytes, being structurally separated from circulating CTLs in glomerular capillaries by an exceptionally thick basement membrane.

Original languageEnglish
Article number3
JournalJournal of Hematology and Oncology
Volume7
Issue number1
DOIs
Publication statusPublished - 06-01-2014

Fingerprint

Podocytes
Adoptive Transfer
Cytotoxic T-Lymphocytes
Kidney
Wounds and Injuries
Translational Medical Research
Basement Membrane
Immunotherapy
Leukemia
Neoplasms
Therapeutics

All Science Journal Classification (ASJC) codes

  • Hematology
  • Molecular Biology
  • Oncology
  • Cancer Research

Cite this

Asai, Hiroaki ; Fujiwara, Hiroshi ; Kitazawa, Sohei ; Kobayashi, Naoto ; Ochi, Toshiki ; Miyazaki, Yukihiro ; Ochi, Fumihiro ; Akatsuka, Yoshiki ; Okamoto, Sachiko ; Mineno, Junichi ; Kuzushima, Kiyotaka ; Ikeda, Hiroaki ; Shiku, Hiroshi ; Yasukawa, Masaki. / Adoptive transfer of genetically engineered WT1-specific cytotoxic T lymphocytes does not induce renal injury. In: Journal of Hematology and Oncology. 2014 ; Vol. 7, No. 1.
@article{d0da6266f1174c608e3bc071fd2d4a9a,
title = "Adoptive transfer of genetically engineered WT1-specific cytotoxic T lymphocytes does not induce renal injury",
abstract = "Because WT1 is expressed in leukemia cells, the development of cancer immunotherapy targeting WT1 has been an attractive translational research topic. However, concern of this therapy still remains, since WT1 is abundantly expressed in renal glomerular podocytes. In the present study, we clearly showed that WT1-specific cytotoxic T lymphocytes (CTLs) certainly exerted cytotoxicity against podocytes in vitro; however, they did not damage podocytes in vivo. This might be due to the anatomical localization of podocytes, being structurally separated from circulating CTLs in glomerular capillaries by an exceptionally thick basement membrane.",
author = "Hiroaki Asai and Hiroshi Fujiwara and Sohei Kitazawa and Naoto Kobayashi and Toshiki Ochi and Yukihiro Miyazaki and Fumihiro Ochi and Yoshiki Akatsuka and Sachiko Okamoto and Junichi Mineno and Kiyotaka Kuzushima and Hiroaki Ikeda and Hiroshi Shiku and Masaki Yasukawa",
year = "2014",
month = "1",
day = "6",
doi = "10.1186/1756-8722-7-3",
language = "English",
volume = "7",
journal = "Journal of Hematology and Oncology",
issn = "1756-8722",
publisher = "BioMed Central",
number = "1",

}

Asai, H, Fujiwara, H, Kitazawa, S, Kobayashi, N, Ochi, T, Miyazaki, Y, Ochi, F, Akatsuka, Y, Okamoto, S, Mineno, J, Kuzushima, K, Ikeda, H, Shiku, H & Yasukawa, M 2014, 'Adoptive transfer of genetically engineered WT1-specific cytotoxic T lymphocytes does not induce renal injury', Journal of Hematology and Oncology, vol. 7, no. 1, 3. https://doi.org/10.1186/1756-8722-7-3

Adoptive transfer of genetically engineered WT1-specific cytotoxic T lymphocytes does not induce renal injury. / Asai, Hiroaki; Fujiwara, Hiroshi; Kitazawa, Sohei; Kobayashi, Naoto; Ochi, Toshiki; Miyazaki, Yukihiro; Ochi, Fumihiro; Akatsuka, Yoshiki; Okamoto, Sachiko; Mineno, Junichi; Kuzushima, Kiyotaka; Ikeda, Hiroaki; Shiku, Hiroshi; Yasukawa, Masaki.

In: Journal of Hematology and Oncology, Vol. 7, No. 1, 3, 06.01.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Adoptive transfer of genetically engineered WT1-specific cytotoxic T lymphocytes does not induce renal injury

AU - Asai, Hiroaki

AU - Fujiwara, Hiroshi

AU - Kitazawa, Sohei

AU - Kobayashi, Naoto

AU - Ochi, Toshiki

AU - Miyazaki, Yukihiro

AU - Ochi, Fumihiro

AU - Akatsuka, Yoshiki

AU - Okamoto, Sachiko

AU - Mineno, Junichi

AU - Kuzushima, Kiyotaka

AU - Ikeda, Hiroaki

AU - Shiku, Hiroshi

AU - Yasukawa, Masaki

PY - 2014/1/6

Y1 - 2014/1/6

N2 - Because WT1 is expressed in leukemia cells, the development of cancer immunotherapy targeting WT1 has been an attractive translational research topic. However, concern of this therapy still remains, since WT1 is abundantly expressed in renal glomerular podocytes. In the present study, we clearly showed that WT1-specific cytotoxic T lymphocytes (CTLs) certainly exerted cytotoxicity against podocytes in vitro; however, they did not damage podocytes in vivo. This might be due to the anatomical localization of podocytes, being structurally separated from circulating CTLs in glomerular capillaries by an exceptionally thick basement membrane.

AB - Because WT1 is expressed in leukemia cells, the development of cancer immunotherapy targeting WT1 has been an attractive translational research topic. However, concern of this therapy still remains, since WT1 is abundantly expressed in renal glomerular podocytes. In the present study, we clearly showed that WT1-specific cytotoxic T lymphocytes (CTLs) certainly exerted cytotoxicity against podocytes in vitro; however, they did not damage podocytes in vivo. This might be due to the anatomical localization of podocytes, being structurally separated from circulating CTLs in glomerular capillaries by an exceptionally thick basement membrane.

UR - http://www.scopus.com/inward/record.url?scp=84891528521&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891528521&partnerID=8YFLogxK

U2 - 10.1186/1756-8722-7-3

DO - 10.1186/1756-8722-7-3

M3 - Article

VL - 7

JO - Journal of Hematology and Oncology

JF - Journal of Hematology and Oncology

SN - 1756-8722

IS - 1

M1 - 3

ER -