Aggravation of brain injury after transient focal ischemia in p53-deficient mice

Keiichiro Maeda, Ryuji Hata, Frank Gillardon, Konstantin Alexander Hossmann

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

The transcriptional factor p53 is a regulatory protein which contributes to the preservation of tissue integrity by promoting either DNA repair or apoptosis. To establish the pathophysiological role of this protein in ischemia, we produced 1 h transient middle cerebral artery (MCA) occlusion in normal and in p53-deficient mice and investigated the resulting tissue damage by multiparametric imaging. Possible genetic influences on the angioarchitecture of the MCA territory and blood flow were examined by intravascular latex infusion and laser-Doppler flowmetry. Wild-type (p53+/+), heterozygous (p53+/-) and homozygous (p53-/-) mice deficient for the p53 gene did not differ in respect to angioarchitecture or the effect of vascular occlusion on blood flow and general physiological parameters. Twenty-four hours after 1 h MCA occlusion, mice revealed a gene dose-dependent decline in the size of metabolic disturbances (ATP depletion and inhibition of protein synthesis) and histological injury (Cresyl Violet staining). DNA fragmentations detected by terminal deoxynucleotidyl transferase-mediated UTP nick end labeling (TUNEL) did not differ in the three groups and were only present in ATP-depleted tissue. Our findings suggest that after transient focal brain ischemia p53 prevents rather than aggravates brain injury, and that this effect is brought about by mechanisms that are unrelated to the pro-apoptotic properties of this gene.

Original languageEnglish
Pages (from-to)54-61
Number of pages8
JournalMolecular Brain Research
Volume88
Issue number1-2
DOIs
Publication statusPublished - 31-03-2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Fingerprint

Dive into the research topics of 'Aggravation of brain injury after transient focal ischemia in p53-deficient mice'. Together they form a unique fingerprint.

Cite this