Aichi virus 2A protein is involved in viral RNA replication

Jun Sasaki, Koki Taniguchi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The Aichi virus 2A protein is not a protease, unlike many other picornavirus 2A proteins, and it is related to a cellular protein, H-rev107. Here, we examined the replication properties of two 2A mutants in Vero cells and a cell-free translation/replication system. In one mutant, amino acids 36 to 126 were replaced with an unrelated amino acid sequence. In the other mutant, the NC motif conserved in the H-rev107 family of proteins was changed to alanine residues. The two mutations abolished virus replication in cells. The mutations affected both negative- and positive-strand synthesis, the defect in positive-strand synthesis being more severe than that in negative-strand synthesis.

Original languageEnglish
Pages (from-to)9765-9769
Number of pages5
JournalJournal of Virology
Volume82
Issue number19
DOIs
Publication statusPublished - 01-10-2008

Fingerprint

Aichi virus
Kobuvirus
Viral RNA
mutants
synthesis
Proteins
proteins
Picornaviridae
mutation
Mutation
Vero Cells
cells
Virus Replication
virus replication
Alanine
alanine
translation (genetics)
Amino Acid Sequence
Peptide Hydrolases
proteinases

All Science Journal Classification (ASJC) codes

  • Immunology
  • Virology

Cite this

Sasaki, Jun ; Taniguchi, Koki. / Aichi virus 2A protein is involved in viral RNA replication. In: Journal of Virology. 2008 ; Vol. 82, No. 19. pp. 9765-9769.
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Aichi virus 2A protein is involved in viral RNA replication. / Sasaki, Jun; Taniguchi, Koki.

In: Journal of Virology, Vol. 82, No. 19, 01.10.2008, p. 9765-9769.

Research output: Contribution to journalArticle

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