Aichi virus 2A protein is involved in viral RNA replication

Jun Sasaki, Koki Taniguchi

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


The Aichi virus 2A protein is not a protease, unlike many other picornavirus 2A proteins, and it is related to a cellular protein, H-rev107. Here, we examined the replication properties of two 2A mutants in Vero cells and a cell-free translation/replication system. In one mutant, amino acids 36 to 126 were replaced with an unrelated amino acid sequence. In the other mutant, the NC motif conserved in the H-rev107 family of proteins was changed to alanine residues. The two mutations abolished virus replication in cells. The mutations affected both negative- and positive-strand synthesis, the defect in positive-strand synthesis being more severe than that in negative-strand synthesis.

Original languageEnglish
Pages (from-to)9765-9769
Number of pages5
JournalJournal of Virology
Issue number19
Publication statusPublished - 10-2008

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


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