TY - JOUR
T1 - Aichi virus 2A protein is involved in viral RNA replication
AU - Sasaki, Jun
AU - Taniguchi, Koki
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2008/10
Y1 - 2008/10
N2 - The Aichi virus 2A protein is not a protease, unlike many other picornavirus 2A proteins, and it is related to a cellular protein, H-rev107. Here, we examined the replication properties of two 2A mutants in Vero cells and a cell-free translation/replication system. In one mutant, amino acids 36 to 126 were replaced with an unrelated amino acid sequence. In the other mutant, the NC motif conserved in the H-rev107 family of proteins was changed to alanine residues. The two mutations abolished virus replication in cells. The mutations affected both negative- and positive-strand synthesis, the defect in positive-strand synthesis being more severe than that in negative-strand synthesis.
AB - The Aichi virus 2A protein is not a protease, unlike many other picornavirus 2A proteins, and it is related to a cellular protein, H-rev107. Here, we examined the replication properties of two 2A mutants in Vero cells and a cell-free translation/replication system. In one mutant, amino acids 36 to 126 were replaced with an unrelated amino acid sequence. In the other mutant, the NC motif conserved in the H-rev107 family of proteins was changed to alanine residues. The two mutations abolished virus replication in cells. The mutations affected both negative- and positive-strand synthesis, the defect in positive-strand synthesis being more severe than that in negative-strand synthesis.
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U2 - 10.1128/JVI.01051-08
DO - 10.1128/JVI.01051-08
M3 - Article
C2 - 18653460
AN - SCOPUS:52649140036
VL - 82
SP - 9765
EP - 9769
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 19
ER -