Aichi virus leader protein is involved in viral RNA replication and encapsidation

Jun Sasaki, Shigeo Nagashima, Koki Taniguchi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Aichi virus, a member of the family Picornaviridae, encodes a leader (L) protein of 170 amino acids (aa). The Aichi virus L protein exhibits no significant sequence homology to those of other picornaviruses. In this study, we investigated the function of the Aichi virus L protein in virus growth. In vitro translation and cleavage assays indicated that the L protein has no autocatalytic activity and is not involved in polyprotein cleavage. The L-VP0 junction was cleaved by 3C proteinase. Immunoblot analysis showed that the L protein is stably present in infected cells. Characterization of various L mutants derived from an infectious cDNA clone revealed that deletion of 93 aa of the center part (aa 43 to 135), 50 aa of the N-terminal part (aa 4 to 53), or 90 aa of the C-terminal part (aa 74 to 163) abolished viral RNA replication. A mutant (Δ114-163) in which 50 aa of the C-terminal part (aa 114 to 163) were deleted exhibited efficient RNA replication and translation abilities, but the virus yield was 4 log orders lower than that of the wild type. Sedimentation analysis of viral particles generated in mutant Δ114-163 RNA-transfected cells showed that the mutant has a severe defect in the formation of mature virions, but not in that of empty capsids. Thus, the data obtained in this study indicate that the Aichi virus L protein is involved in both viral RNA replication and encapsidation.

Original languageEnglish
Pages (from-to)10799-10807
Number of pages9
JournalJournal of Virology
Volume77
Issue number20
DOIs
Publication statusPublished - 01-10-2003

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Aichi virus
Kobuvirus
Viral RNA
Amino Acids
amino acids
Proteins
proteins
Picornaviridae
mutants
virion
Virion
translation (genetics)
RNA
Viruses
RNA replication
Polyproteins
viruses
Aptitude
capsid
Capsid

All Science Journal Classification (ASJC) codes

  • Immunology

Cite this

Sasaki, Jun ; Nagashima, Shigeo ; Taniguchi, Koki. / Aichi virus leader protein is involved in viral RNA replication and encapsidation. In: Journal of Virology. 2003 ; Vol. 77, No. 20. pp. 10799-10807.
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Aichi virus leader protein is involved in viral RNA replication and encapsidation. / Sasaki, Jun; Nagashima, Shigeo; Taniguchi, Koki.

In: Journal of Virology, Vol. 77, No. 20, 01.10.2003, p. 10799-10807.

Research output: Contribution to journalArticle

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