Allergen analysis of sea urchin roe using sera from five patients

Kenichi Tanaka, Yasuto Kondo, Chisato Inuo, Yoichi Nakajima, Ikuya Tsuge, Satoru Doi, Shigeto Yanagihara, Tetsushi Yoshikawa, Atsuo Urisu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Sea urchin roe can cause anaphylactic reactions the first time they are consumed; therefore, careful clinical attention should be paid to their effects. However, no previous study has examined the allergens in sea urchin roe using sera from more than one patient. We attempted to identify sea urchin allergens using sera from 5 patients with sea urchin allergies. Methods: We enrolled 5 patients with relevant medical histories, positive results on a skin prick test and/or a food challenge test, and high levels of sea urchinspecific IgE in an enzyme-linked immunosorbent assay. We performed SDS-PAGE, immunoblotting, immunoblot inhibition, and N-terminal amino acid sequence detection. Results: Ten protein bands ranging from 18 to 170 kDa were detected in more than 2 patients' sera. In immunoblotting, the protein band for the 170-kDa major yolk protein was recognized by 4 of the 5 sera. Furthermore, the reaction between IgE and the protein band for egg cortical vesicle protein (18 kDa) was inhibited by the addition of salmon roe extract. Conclusion: Major yolk protein was confirmed to beone of the main allergens in sea urchin roe. In addition, egg cortical vesicle protein (18 kDa) was demonstrated to be an important protein for cross-reactivity with salmon roe.

Original languageEnglish
Pages (from-to)222-227
Number of pages6
JournalInternational Archives of Allergy and Immunology
Volume164
Issue number3
DOIs
Publication statusPublished - 07-11-2014

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Sea Urchins
Allergens
Egg Proteins
Serum
Salmon
Proteins
Immunoblotting
Immunoglobulin E
Anaphylaxis
Skin Tests
Oceans and Seas
Ovum
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Hypersensitivity
Enzyme-Linked Immunosorbent Assay
Food

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Tanaka, Kenichi ; Kondo, Yasuto ; Inuo, Chisato ; Nakajima, Yoichi ; Tsuge, Ikuya ; Doi, Satoru ; Yanagihara, Shigeto ; Yoshikawa, Tetsushi ; Urisu, Atsuo. / Allergen analysis of sea urchin roe using sera from five patients. In: International Archives of Allergy and Immunology. 2014 ; Vol. 164, No. 3. pp. 222-227.
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Allergen analysis of sea urchin roe using sera from five patients. / Tanaka, Kenichi; Kondo, Yasuto; Inuo, Chisato; Nakajima, Yoichi; Tsuge, Ikuya; Doi, Satoru; Yanagihara, Shigeto; Yoshikawa, Tetsushi; Urisu, Atsuo.

In: International Archives of Allergy and Immunology, Vol. 164, No. 3, 07.11.2014, p. 222-227.

Research output: Contribution to journalArticle

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AU - Tanaka, Kenichi

AU - Kondo, Yasuto

AU - Inuo, Chisato

AU - Nakajima, Yoichi

AU - Tsuge, Ikuya

AU - Doi, Satoru

AU - Yanagihara, Shigeto

AU - Yoshikawa, Tetsushi

AU - Urisu, Atsuo

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N2 - Background: Sea urchin roe can cause anaphylactic reactions the first time they are consumed; therefore, careful clinical attention should be paid to their effects. However, no previous study has examined the allergens in sea urchin roe using sera from more than one patient. We attempted to identify sea urchin allergens using sera from 5 patients with sea urchin allergies. Methods: We enrolled 5 patients with relevant medical histories, positive results on a skin prick test and/or a food challenge test, and high levels of sea urchinspecific IgE in an enzyme-linked immunosorbent assay. We performed SDS-PAGE, immunoblotting, immunoblot inhibition, and N-terminal amino acid sequence detection. Results: Ten protein bands ranging from 18 to 170 kDa were detected in more than 2 patients' sera. In immunoblotting, the protein band for the 170-kDa major yolk protein was recognized by 4 of the 5 sera. Furthermore, the reaction between IgE and the protein band for egg cortical vesicle protein (18 kDa) was inhibited by the addition of salmon roe extract. Conclusion: Major yolk protein was confirmed to beone of the main allergens in sea urchin roe. In addition, egg cortical vesicle protein (18 kDa) was demonstrated to be an important protein for cross-reactivity with salmon roe.

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