TY - JOUR
T1 - Alterations in multiple tyrosine hydroxylase mrnas in the substantia nigra, locus coeruleus and adrenal gland of MPTP-treated parkinsonian monkeys
AU - Ohye, T.
AU - Ichinose, H.
AU - Ogawa, M.
AU - Yoshida, M.
AU - Nagatsu, T.
N1 - Funding Information:
This work was supported by Grant-in-Aid from the Ministry of Education, Science and Culture of Japan (to T. Nagatsu and M. Yoshida) and a Grant-in-Aid from Fujita Health University.
PY - 1995/3
Y1 - 1995/3
N2 - Monkeys are known to be highly susceptible to 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) which produces parkinsonism, as in humans. We have previously reported that only monkeys and humans have multiple isoforms of tyrosine hydroxylase (TH), the first enzyme for dopamine biosynthesis, with only two TH mRNA isoforms, type 1 and type 2, being present inMacaca fascicularis. In the present study we have measured TH mRNA type 1 and 2 content in the substantia nigra, locus coeruleus, and adrenal gland of normal control monkeys and in MPTP-produced parkinsonian monkeys (Macaca fascicularis) using a newly developed, sensitive and quantitative assay based on the reverse transcription-polymerase chain reaction. Marked decreases in TH mRNA type 1 and 2 content were observed, specifically in the substantia nigra of the monkeys with MPTP-parkinsonism compared to control monkeys. These results are similar to our recent data showing marked decreases in TH mRNA type 1, 2, 3 and 4 content in the substantia nigra of patients with Parkinson’s disease, and suggest that MPTP-treated monkeys closely replicate changes in TH isoforms in human Parkinson’s disease.
AB - Monkeys are known to be highly susceptible to 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) which produces parkinsonism, as in humans. We have previously reported that only monkeys and humans have multiple isoforms of tyrosine hydroxylase (TH), the first enzyme for dopamine biosynthesis, with only two TH mRNA isoforms, type 1 and type 2, being present inMacaca fascicularis. In the present study we have measured TH mRNA type 1 and 2 content in the substantia nigra, locus coeruleus, and adrenal gland of normal control monkeys and in MPTP-produced parkinsonian monkeys (Macaca fascicularis) using a newly developed, sensitive and quantitative assay based on the reverse transcription-polymerase chain reaction. Marked decreases in TH mRNA type 1 and 2 content were observed, specifically in the substantia nigra of the monkeys with MPTP-parkinsonism compared to control monkeys. These results are similar to our recent data showing marked decreases in TH mRNA type 1, 2, 3 and 4 content in the substantia nigra of patients with Parkinson’s disease, and suggest that MPTP-treated monkeys closely replicate changes in TH isoforms in human Parkinson’s disease.
UR - http://www.scopus.com/inward/record.url?scp=0029077787&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029077787&partnerID=8YFLogxK
U2 - 10.1006/neur.1995.0009
DO - 10.1006/neur.1995.0009
M3 - Article
C2 - 7600187
AN - SCOPUS:0029077787
SN - 1055-8330
VL - 4
SP - 81
EP - 85
JO - Neurodegeneration
JF - Neurodegeneration
IS - 1
ER -
