Alterations in multiple tyrosine hydroxylase mrnas in the substantia nigra, locus coeruleus and adrenal gland of MPTP-treated parkinsonian monkeys

Tamae Oe, H. Ichinose, M. Ogawa, M. Yoshida, T. Nagatsu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Monkeys are known to be highly susceptible to 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) which produces parkinsonism, as in humans. We have previously reported that only monkeys and humans have multiple isoforms of tyrosine hydroxylase (TH), the first enzyme for dopamine biosynthesis, with only two TH mRNA isoforms, type 1 and type 2, being present inMacaca fascicularis. In the present study we have measured TH mRNA type 1 and 2 content in the substantia nigra, locus coeruleus, and adrenal gland of normal control monkeys and in MPTP-produced parkinsonian monkeys (Macaca fascicularis) using a newly developed, sensitive and quantitative assay based on the reverse transcription-polymerase chain reaction. Marked decreases in TH mRNA type 1 and 2 content were observed, specifically in the substantia nigra of the monkeys with MPTP-parkinsonism compared to control monkeys. These results are similar to our recent data showing marked decreases in TH mRNA type 1, 2, 3 and 4 content in the substantia nigra of patients with Parkinson’s disease, and suggest that MPTP-treated monkeys closely replicate changes in TH isoforms in human Parkinson’s disease.

Original languageEnglish
Pages (from-to)81-85
Number of pages5
JournalNeurodegeneration
Volume4
Issue number1
DOIs
Publication statusPublished - 01-01-1995

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1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Locus Coeruleus
Tyrosine 3-Monooxygenase
Substantia Nigra
Adrenal Glands
Haplorhini
Parkinsonian Disorders
Messenger RNA
Parkinson Disease
Protein Isoforms
RNA Isoforms
Macaca fascicularis
Reverse Transcription
Dopamine
Polymerase Chain Reaction
Enzymes

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Neuropsychology and Physiological Psychology
  • Clinical Neurology

Cite this

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abstract = "Monkeys are known to be highly susceptible to 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) which produces parkinsonism, as in humans. We have previously reported that only monkeys and humans have multiple isoforms of tyrosine hydroxylase (TH), the first enzyme for dopamine biosynthesis, with only two TH mRNA isoforms, type 1 and type 2, being present inMacaca fascicularis. In the present study we have measured TH mRNA type 1 and 2 content in the substantia nigra, locus coeruleus, and adrenal gland of normal control monkeys and in MPTP-produced parkinsonian monkeys (Macaca fascicularis) using a newly developed, sensitive and quantitative assay based on the reverse transcription-polymerase chain reaction. Marked decreases in TH mRNA type 1 and 2 content were observed, specifically in the substantia nigra of the monkeys with MPTP-parkinsonism compared to control monkeys. These results are similar to our recent data showing marked decreases in TH mRNA type 1, 2, 3 and 4 content in the substantia nigra of patients with Parkinson’s disease, and suggest that MPTP-treated monkeys closely replicate changes in TH isoforms in human Parkinson’s disease.",
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Alterations in multiple tyrosine hydroxylase mrnas in the substantia nigra, locus coeruleus and adrenal gland of MPTP-treated parkinsonian monkeys. / Oe, Tamae; Ichinose, H.; Ogawa, M.; Yoshida, M.; Nagatsu, T.

In: Neurodegeneration, Vol. 4, No. 1, 01.01.1995, p. 81-85.

Research output: Contribution to journalArticle

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