Altered extracellular matrix component gene expression in murine polycystic kidney

I. Ebihara, T. Nakamura, T. Takahashi, M. Yamamoto, Y. Tomino, Shizuko Nagao, H. Takahashi, H. Koide

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Abstract

The DBA/2FG-pcy mouse has a form of slowly progressive kidney disease that appears similar in many respects to that seen in the autosomal dominant form of human polycystic kidney disease. This study was designed to assess how the expression of extracellular matrix component genes is regulated in a model of murine polycystic kidney disease and control DBA/2 mice at 8, 16, and 30 weeks of age. The mRNA levels encoding for collagen IV, the B1 and B2 chains of laminin, heparan sulfate proteoglycan, fibronectin, and collagens I and III increased with the progression of cystic lesions in the kidney of DBA/2FG-pcy mice. At 30 weeks of age, mRNA levels for collagen IV, laminin B1 and B2, heparan sulfate proteoglycan, fibronectin, and collagens I and III were increased 8.1-fold, 7.0-fold, 7.0-fold, 9.8-fold, 7.0-fold, 5.5-fold, and 5.4-fold, respectively, compared to those of control DBA/2 mice. An immunofluorescence study revealed the irregular staining for collagen IV; laminin, heparan sulfate proteoglycan, and collagens I and III around the cysts. These data suggest that changes in the expression of basement membrane components and interstitial collagens are associated with the development of polycystic kidney disease.

Original languageEnglish
Pages (from-to)73-80
Number of pages8
JournalRenal Physiology and Biochemistry
Volume18
Issue number2
Publication statusPublished - 01-01-1995
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Physiology
  • Nephrology

Cite this

Ebihara, I., Nakamura, T., Takahashi, T., Yamamoto, M., Tomino, Y., Nagao, S., Takahashi, H., & Koide, H. (1995). Altered extracellular matrix component gene expression in murine polycystic kidney. Renal Physiology and Biochemistry, 18(2), 73-80.