Altered gene expression in lymphoblastoid cell lines after subculture

Michihiro Toritsuka; Manabu Makinodan; Takahira Yamauchi; Yasunori Yamashita; Daisuke Ikawa; Takashi Komori; Sohei Kimoto; Kaori Hamano-Iwasa; Hideo Matsuzaki; Toshifumi Kishimoto

doi:10.1007/s11626-018-0267-1

Altered gene expression in lymphoblastoid cell lines after subculture

Michihiro Toritsuka
, Manabu Makinodan
, Takahira Yamauchi
, Yasunori Yamashita
, Daisuke Ikawa
, Takashi Komori
, Sohei Kimoto
, Kaori Hamano-Iwasa
, Hideo Matsuzaki
, Toshifumi Kishimoto

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Lymphoblastoid cell lines (LCLs) are nearly immortalized B lymphocytes that are used as long-lasting supply of human cells for studies on gene expression analyses. However, studies on the stability of the cellular features of LCLs are scarce. To address this issue, we measured gene expression in LCLs with different passage numbers and observed that gene expression substantially changed within 10 passages. In particular, the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a well-known housekeeping gene, varied considerably during subculture; thus, the use GAPDH as an internal control may be unsuitable. In conclusion, this study highlights the need for exercising caution during determination of gene expression in LCLs.

Original language	English
Pages (from-to)	523-527
Number of pages	5
Journal	In Vitro Cellular and Developmental Biology - Animal
Volume	54
Issue number	7
DOIs	https://doi.org/10.1007/s11626-018-0267-1
Publication status	Published - 01-08-2018
Externally published	Yes

All Science Journal Classification (ASJC) codes

Developmental Biology
Cell Biology

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10.1007/s11626-018-0267-1

Cite this

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title = "Altered gene expression in lymphoblastoid cell lines after subculture",

abstract = "Lymphoblastoid cell lines (LCLs) are nearly immortalized B lymphocytes that are used as long-lasting supply of human cells for studies on gene expression analyses. However, studies on the stability of the cellular features of LCLs are scarce. To address this issue, we measured gene expression in LCLs with different passage numbers and observed that gene expression substantially changed within 10 passages. In particular, the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a well-known housekeeping gene, varied considerably during subculture; thus, the use GAPDH as an internal control may be unsuitable. In conclusion, this study highlights the need for exercising caution during determination of gene expression in LCLs.",

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doi = "10.1007/s11626-018-0267-1",

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journal = "In Vitro Cellular and Developmental Biology - Animal",

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TY - JOUR

T1 - Altered gene expression in lymphoblastoid cell lines after subculture

AU - Toritsuka, Michihiro

AU - Makinodan, Manabu

AU - Yamauchi, Takahira

AU - Yamashita, Yasunori

AU - Ikawa, Daisuke

AU - Komori, Takashi

AU - Kimoto, Sohei

AU - Hamano-Iwasa, Kaori

AU - Matsuzaki, Hideo

AU - Kishimoto, Toshifumi

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Lymphoblastoid cell lines (LCLs) are nearly immortalized B lymphocytes that are used as long-lasting supply of human cells for studies on gene expression analyses. However, studies on the stability of the cellular features of LCLs are scarce. To address this issue, we measured gene expression in LCLs with different passage numbers and observed that gene expression substantially changed within 10 passages. In particular, the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a well-known housekeeping gene, varied considerably during subculture; thus, the use GAPDH as an internal control may be unsuitable. In conclusion, this study highlights the need for exercising caution during determination of gene expression in LCLs.

AB - Lymphoblastoid cell lines (LCLs) are nearly immortalized B lymphocytes that are used as long-lasting supply of human cells for studies on gene expression analyses. However, studies on the stability of the cellular features of LCLs are scarce. To address this issue, we measured gene expression in LCLs with different passage numbers and observed that gene expression substantially changed within 10 passages. In particular, the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a well-known housekeeping gene, varied considerably during subculture; thus, the use GAPDH as an internal control may be unsuitable. In conclusion, this study highlights the need for exercising caution during determination of gene expression in LCLs.

KW - Cytokine

KW - GAPDH

KW - Lymphoblastoid cell line

KW - Subculture

UR - https://www.scopus.com/pages/publications/85048357047

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U2 - 10.1007/s11626-018-0267-1

DO - 10.1007/s11626-018-0267-1

M3 - Article

C2 - 29948745

AN - SCOPUS:85048357047

SN - 1071-2690

VL - 54

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EP - 527

JO - In Vitro Cellular and Developmental Biology - Animal

JF - In Vitro Cellular and Developmental Biology - Animal

IS - 7

ER -

Altered gene expression in lymphoblastoid cell lines after subculture

Abstract

All Science Journal Classification (ASJC) codes

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