Altered sensitivities to morphine and cocaine in scaffold protein tamalin knockout mice

Masaaki Ogawa, Tsuyoshi Miyakawa, Kenji Nakamura, Jun Kitano, Kenryo Furushima, Hiroshi Kiyonari, Rika Nakayama, Kazuki Nakao, Koki Moriyoshi, Shigetada Nakanishi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Tamalin is a scaffold protein that interacts with metabotropic glutamate receptors and the kinase-deficient neurotrophin TrkCT1 receptor and forms a protein complex with multiple protein-trafficking and intracellular signaling molecules. In culture, tamalin promotes intracellular trafficking of group 1 metabotropic glutamate receptors through its interaction with guanine nucleotide exchange factor cytohesins and causes actin reorganization and membrane ruffling via the TrkCT1/cytohesin-2 signaling mechanism. However, how tamalin serves its physiological function in vivo has remained elusive. In this study, we generated tamalin knockout (Tam-/- KO) mice and investigated behavioral alterations resulting from their deficiency in functional tamalin. Targeted deletion of functional tamalin altered neither the overall brain architecture nor the general behavior of the mice under ordinary conditions. However, Tam-/- KO mice showed a decrease in sensitivity to acute morphine-induced hyperlocomotion and morphine analgesic effects in the hot-plate test. Furthermore, tamalin deficiency impaired the ability of the animals to show conditioned place preference after repeated morphine administration and to display locomotor sensitization by chronic cocaine treatment. Upon in vivo microdialysis analysis of the nucleus accumbens, Tam-/- KO and wild-type mice showed no genotypic differences in their response patterns of extracellular dopamine and glutamate before or after morphine administration. These results demonstrate that the tamalin scaffold protein plays a unique role in both acute and adaptive behavioral responses to morphine and cocaine and could regulate common neural substrates implicated in drugs of abuse.

Original languageEnglish
Pages (from-to)14789-14794
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number37
DOIs
Publication statusPublished - 11-09-2007

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Cocaine
Knockout Mice
Morphine
glutamate 5-kinase
Proteins
Nerve Growth Factor Receptors
Guanine Nucleotide Exchange Factors
Metabotropic Glutamate Receptors
Microdialysis
Nucleus Accumbens
Street Drugs
Protein Transport
Analgesics
Actins
Glutamic Acid
Dopamine
Membranes
Brain

All Science Journal Classification (ASJC) codes

  • General

Cite this

Ogawa, Masaaki ; Miyakawa, Tsuyoshi ; Nakamura, Kenji ; Kitano, Jun ; Furushima, Kenryo ; Kiyonari, Hiroshi ; Nakayama, Rika ; Nakao, Kazuki ; Moriyoshi, Koki ; Nakanishi, Shigetada. / Altered sensitivities to morphine and cocaine in scaffold protein tamalin knockout mice. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 37. pp. 14789-14794.
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Ogawa, M, Miyakawa, T, Nakamura, K, Kitano, J, Furushima, K, Kiyonari, H, Nakayama, R, Nakao, K, Moriyoshi, K & Nakanishi, S 2007, 'Altered sensitivities to morphine and cocaine in scaffold protein tamalin knockout mice', Proceedings of the National Academy of Sciences of the United States of America, vol. 104, no. 37, pp. 14789-14794. https://doi.org/10.1073/pnas.0706945104

Altered sensitivities to morphine and cocaine in scaffold protein tamalin knockout mice. / Ogawa, Masaaki; Miyakawa, Tsuyoshi; Nakamura, Kenji; Kitano, Jun; Furushima, Kenryo; Kiyonari, Hiroshi; Nakayama, Rika; Nakao, Kazuki; Moriyoshi, Koki; Nakanishi, Shigetada.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 37, 11.09.2007, p. 14789-14794.

Research output: Contribution to journalArticle

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T1 - Altered sensitivities to morphine and cocaine in scaffold protein tamalin knockout mice

AU - Ogawa, Masaaki

AU - Miyakawa, Tsuyoshi

AU - Nakamura, Kenji

AU - Kitano, Jun

AU - Furushima, Kenryo

AU - Kiyonari, Hiroshi

AU - Nakayama, Rika

AU - Nakao, Kazuki

AU - Moriyoshi, Koki

AU - Nakanishi, Shigetada

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