Abstract
The mouse HSP47 gene consists of six exons separated by five introns. Three HSP47 cDNAs differing only in their 5′ noncoding regions have been reported. One of these alternatively spliced mRNAs was detected only after heat shock, which caused an alternative 5′ splice donor site selection. Other stress inducers, including an amino acid analog and sodium arsenite, had no effect on the alternative splicing. The alternatively spliced mRNA, which was 169 nucleotides longer in the 5′ noncoding region compared to mRNA transcribed in non-heat shock conditions, was efficiently translated under heat shock conditions. This novel finding that alternative splicing is caused by artificial treatment like heat shock will provide a useful in vivo model for understanding the exon-intron recognition mechanism as well as heat shock-induced alterations in gene expression.
| Original language | English |
|---|---|
| Pages (from-to) | 567-575 |
| Number of pages | 9 |
| Journal | Molecular and Cellular Biology |
| Volume | 14 |
| Issue number | 1 |
| Publication status | Published - 01-12-1994 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Cell Biology
- Genetics
- Molecular Biology
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Alternative 5′ splice site selection induced by heat shock. / Takechi, Hajime; Hosokawa, Nobuko; Hirayoshi, Kazunori; Nagata, Kazuhiro.
In: Molecular and Cellular Biology, Vol. 14, No. 1, 01.12.1994, p. 567-575.Research output: Contribution to journal › Article
TY - JOUR
T1 - Alternative 5′ splice site selection induced by heat shock
AU - Takechi, Hajime
AU - Hosokawa, Nobuko
AU - Hirayoshi, Kazunori
AU - Nagata, Kazuhiro
PY - 1994/12/1
Y1 - 1994/12/1
N2 - The mouse HSP47 gene consists of six exons separated by five introns. Three HSP47 cDNAs differing only in their 5′ noncoding regions have been reported. One of these alternatively spliced mRNAs was detected only after heat shock, which caused an alternative 5′ splice donor site selection. Other stress inducers, including an amino acid analog and sodium arsenite, had no effect on the alternative splicing. The alternatively spliced mRNA, which was 169 nucleotides longer in the 5′ noncoding region compared to mRNA transcribed in non-heat shock conditions, was efficiently translated under heat shock conditions. This novel finding that alternative splicing is caused by artificial treatment like heat shock will provide a useful in vivo model for understanding the exon-intron recognition mechanism as well as heat shock-induced alterations in gene expression.
AB - The mouse HSP47 gene consists of six exons separated by five introns. Three HSP47 cDNAs differing only in their 5′ noncoding regions have been reported. One of these alternatively spliced mRNAs was detected only after heat shock, which caused an alternative 5′ splice donor site selection. Other stress inducers, including an amino acid analog and sodium arsenite, had no effect on the alternative splicing. The alternatively spliced mRNA, which was 169 nucleotides longer in the 5′ noncoding region compared to mRNA transcribed in non-heat shock conditions, was efficiently translated under heat shock conditions. This novel finding that alternative splicing is caused by artificial treatment like heat shock will provide a useful in vivo model for understanding the exon-intron recognition mechanism as well as heat shock-induced alterations in gene expression.
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M3 - Article
VL - 14
SP - 567
EP - 575
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 1
ER -