Alternative 5′ splice site selection induced by heat shock

Hajime Takechi, Nobuko Hosokawa, Kazunori Hirayoshi, Kazuhiro Nagata

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)


The mouse HSP47 gene consists of six exons separated by five introns. Three HSP47 cDNAs differing only in their 5′ noncoding regions have been reported. One of these alternatively spliced mRNAs was detected only after heat shock, which caused an alternative 5′ splice donor site selection. Other stress inducers, including an amino acid analog and sodium arsenite, had no effect on the alternative splicing. The alternatively spliced mRNA, which was 169 nucleotides longer in the 5′ noncoding region compared to mRNA transcribed in non-heat shock conditions, was efficiently translated under heat shock conditions. This novel finding that alternative splicing is caused by artificial treatment like heat shock will provide a useful in vivo model for understanding the exon-intron recognition mechanism as well as heat shock-induced alterations in gene expression.

Original languageEnglish
Pages (from-to)567-575
Number of pages9
JournalMolecular and Cellular Biology
Issue number1
Publication statusPublished - 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


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