Amino acid sequence of a putative transposase protein of the medaka fish transposable element Tol2 deduced from mRNA nucleotide sequences

Akihiko Koga, Miho Suzuki, Yuki Maruyama, Makiko Tsutsumi, Hiroshi Hori

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Tol2 is a terminal-inverted repeat transposable element of the medaka fish Oryzias latipes. It is one of a few elements of this class so far demonstrated to be active in vertebrates, thus providing a unique tool for establishing a gene tagging system. For the purpose of identifying its transposase, we analyzed the structures of mRNAs originating from the Tol2 element. The results indicated that transcription of Tol2 is initiated at several sites, the four open reading frames in Tol2 roughly corresponding to exons, and that two main forms of mRNAs, covering exons 1-4 and exons 2-4, are present in medaka fish cells. One or both of these mRNAs are likely to encode a transposase, the amino acid sequence of which was deduced. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)295-298
Number of pages4
JournalFEBS Letters
Volume461
Issue number3
DOIs
Publication statusPublished - 19-11-1999

Fingerprint

Fish Proteins
Oryzias
Transposases
DNA Transposable Elements
Fish
Amino Acid Sequence
Exons
Nucleotides
Amino Acids
Messenger RNA
Fishes
Proteins
Terminal Repeat Sequences
Transcription
Open Reading Frames
Vertebrates
Genes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

@article{f7b063a1f9314982ae1618800915b68b,
title = "Amino acid sequence of a putative transposase protein of the medaka fish transposable element Tol2 deduced from mRNA nucleotide sequences",
abstract = "Tol2 is a terminal-inverted repeat transposable element of the medaka fish Oryzias latipes. It is one of a few elements of this class so far demonstrated to be active in vertebrates, thus providing a unique tool for establishing a gene tagging system. For the purpose of identifying its transposase, we analyzed the structures of mRNAs originating from the Tol2 element. The results indicated that transcription of Tol2 is initiated at several sites, the four open reading frames in Tol2 roughly corresponding to exons, and that two main forms of mRNAs, covering exons 1-4 and exons 2-4, are present in medaka fish cells. One or both of these mRNAs are likely to encode a transposase, the amino acid sequence of which was deduced. Copyright (C) 1999 Federation of European Biochemical Societies.",
author = "Akihiko Koga and Miho Suzuki and Yuki Maruyama and Makiko Tsutsumi and Hiroshi Hori",
year = "1999",
month = "11",
day = "19",
doi = "10.1016/S0014-5793(99)01479-9",
language = "English",
volume = "461",
pages = "295--298",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

Amino acid sequence of a putative transposase protein of the medaka fish transposable element Tol2 deduced from mRNA nucleotide sequences. / Koga, Akihiko; Suzuki, Miho; Maruyama, Yuki; Tsutsumi, Makiko; Hori, Hiroshi.

In: FEBS Letters, Vol. 461, No. 3, 19.11.1999, p. 295-298.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Amino acid sequence of a putative transposase protein of the medaka fish transposable element Tol2 deduced from mRNA nucleotide sequences

AU - Koga, Akihiko

AU - Suzuki, Miho

AU - Maruyama, Yuki

AU - Tsutsumi, Makiko

AU - Hori, Hiroshi

PY - 1999/11/19

Y1 - 1999/11/19

N2 - Tol2 is a terminal-inverted repeat transposable element of the medaka fish Oryzias latipes. It is one of a few elements of this class so far demonstrated to be active in vertebrates, thus providing a unique tool for establishing a gene tagging system. For the purpose of identifying its transposase, we analyzed the structures of mRNAs originating from the Tol2 element. The results indicated that transcription of Tol2 is initiated at several sites, the four open reading frames in Tol2 roughly corresponding to exons, and that two main forms of mRNAs, covering exons 1-4 and exons 2-4, are present in medaka fish cells. One or both of these mRNAs are likely to encode a transposase, the amino acid sequence of which was deduced. Copyright (C) 1999 Federation of European Biochemical Societies.

AB - Tol2 is a terminal-inverted repeat transposable element of the medaka fish Oryzias latipes. It is one of a few elements of this class so far demonstrated to be active in vertebrates, thus providing a unique tool for establishing a gene tagging system. For the purpose of identifying its transposase, we analyzed the structures of mRNAs originating from the Tol2 element. The results indicated that transcription of Tol2 is initiated at several sites, the four open reading frames in Tol2 roughly corresponding to exons, and that two main forms of mRNAs, covering exons 1-4 and exons 2-4, are present in medaka fish cells. One or both of these mRNAs are likely to encode a transposase, the amino acid sequence of which was deduced. Copyright (C) 1999 Federation of European Biochemical Societies.

UR - http://www.scopus.com/inward/record.url?scp=0032740435&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032740435&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(99)01479-9

DO - 10.1016/S0014-5793(99)01479-9

M3 - Article

VL - 461

SP - 295

EP - 298

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -