Amperometric fructose sensor based on direct bioelectrocatalysis

Tokuji Ikeda, Fumio Matsushita, Mitsugi Senda

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110 Citations (Scopus)

Abstract

Fructose dehydrogenase (EC 1.1.99.11) from bacterial membranes was immobilized on a carbon paste electrode by covering the enzyme layer with a dialysis membrane. The fructose dehydrogenase-modified carbon paste electrode showed a current response to d-fructose without the addition of any external electron transfer mediators. The current response was independent of the oxygen concentration in the solution. Steady-state currents were obtained when measured at fixed electrode potentials. The dependence of the steady-state current on the potential, the pH of the solution and the temperature was studied. On the basis of this investigation, it was shown that the fructose dehydrogenase-modified carbon paste electrode could be used as an unmediated amperometric fructose sensor. d-Fructose in fruits was measured using the present electrode. A method of eliminating the effect of l-ascorbic acid is also described.

Original languageEnglish
Pages (from-to)299-304
Number of pages6
JournalBiosensors and Bioelectronics
Volume6
Issue number4
DOIs
Publication statusPublished - 1991

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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