Amperometric fructose sensor based on direct bioelectrocatalysis

Tokuji Ikeda; Fumio Matsushita; Mitsugi Senda

doi:10.1016/0956-5663(91)85015-O

Amperometric fructose sensor based on direct bioelectrocatalysis

Tokuji Ikeda, Fumio Matsushita, Mitsugi Senda

Research output: Contribution to journal › Article › peer-review

114 Citations (Scopus)

Abstract

Fructose dehydrogenase (EC 1.1.99.11) from bacterial membranes was immobilized on a carbon paste electrode by covering the enzyme layer with a dialysis membrane. The fructose dehydrogenase-modified carbon paste electrode showed a current response to d-fructose without the addition of any external electron transfer mediators. The current response was independent of the oxygen concentration in the solution. Steady-state currents were obtained when measured at fixed electrode potentials. The dependence of the steady-state current on the potential, the pH of the solution and the temperature was studied. On the basis of this investigation, it was shown that the fructose dehydrogenase-modified carbon paste electrode could be used as an unmediated amperometric fructose sensor. d-Fructose in fruits was measured using the present electrode. A method of eliminating the effect of l-ascorbic acid is also described.

Original language	English
Pages (from-to)	299-304
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	6
Issue number	4
DOIs	https://doi.org/10.1016/0956-5663(91)85015-O
Publication status	Published - 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/0956-5663(91)85015-O

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title = "Amperometric fructose sensor based on direct bioelectrocatalysis",

abstract = "Fructose dehydrogenase (EC 1.1.99.11) from bacterial membranes was immobilized on a carbon paste electrode by covering the enzyme layer with a dialysis membrane. The fructose dehydrogenase-modified carbon paste electrode showed a current response to d-fructose without the addition of any external electron transfer mediators. The current response was independent of the oxygen concentration in the solution. Steady-state currents were obtained when measured at fixed electrode potentials. The dependence of the steady-state current on the potential, the pH of the solution and the temperature was studied. On the basis of this investigation, it was shown that the fructose dehydrogenase-modified carbon paste electrode could be used as an unmediated amperometric fructose sensor. d-Fructose in fruits was measured using the present electrode. A method of eliminating the effect of l-ascorbic acid is also described.",

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AU - Ikeda, Tokuji

AU - Matsushita, Fumio

AU - Senda, Mitsugi

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Y1 - 1991

N2 - Fructose dehydrogenase (EC 1.1.99.11) from bacterial membranes was immobilized on a carbon paste electrode by covering the enzyme layer with a dialysis membrane. The fructose dehydrogenase-modified carbon paste electrode showed a current response to d-fructose without the addition of any external electron transfer mediators. The current response was independent of the oxygen concentration in the solution. Steady-state currents were obtained when measured at fixed electrode potentials. The dependence of the steady-state current on the potential, the pH of the solution and the temperature was studied. On the basis of this investigation, it was shown that the fructose dehydrogenase-modified carbon paste electrode could be used as an unmediated amperometric fructose sensor. d-Fructose in fruits was measured using the present electrode. A method of eliminating the effect of l-ascorbic acid is also described.

AB - Fructose dehydrogenase (EC 1.1.99.11) from bacterial membranes was immobilized on a carbon paste electrode by covering the enzyme layer with a dialysis membrane. The fructose dehydrogenase-modified carbon paste electrode showed a current response to d-fructose without the addition of any external electron transfer mediators. The current response was independent of the oxygen concentration in the solution. Steady-state currents were obtained when measured at fixed electrode potentials. The dependence of the steady-state current on the potential, the pH of the solution and the temperature was studied. On the basis of this investigation, it was shown that the fructose dehydrogenase-modified carbon paste electrode could be used as an unmediated amperometric fructose sensor. d-Fructose in fruits was measured using the present electrode. A method of eliminating the effect of l-ascorbic acid is also described.

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