Amperometric fructose sensor based on direct bioelectrocatalysis

Tokuji Ikeda, Fumio Matsushita, Mitsugi Senda

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Fructose dehydrogenase (EC 1.1.99.11) from bacterial membranes was immobilized on a carbon paste electrode by covering the enzyme layer with a dialysis membrane. The fructose dehydrogenase-modified carbon paste electrode showed a current response to d-fructose without the addition of any external electron transfer mediators. The current response was independent of the oxygen concentration in the solution. Steady-state currents were obtained when measured at fixed electrode potentials. The dependence of the steady-state current on the potential, the pH of the solution and the temperature was studied. On the basis of this investigation, it was shown that the fructose dehydrogenase-modified carbon paste electrode could be used as an unmediated amperometric fructose sensor. d-Fructose in fruits was measured using the present electrode. A method of eliminating the effect of l-ascorbic acid is also described.

Original languageEnglish
Pages (from-to)299-304
Number of pages6
JournalBiosensors and Bioelectronics
Volume6
Issue number4
DOIs
Publication statusPublished - 01-01-1991

Fingerprint

Fructose
Sensors
Electrodes
Ointments
Oxidoreductases
D-fructose 5-dehydrogenase
Carbon
Dialysis membranes
Membranes
Ascorbic acid
Biosensing Techniques
Fruits
Ascorbic Acid
Dialysis
Fruit
Enzymes
Electrons
Oxygen
Temperature

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Ikeda, Tokuji ; Matsushita, Fumio ; Senda, Mitsugi. / Amperometric fructose sensor based on direct bioelectrocatalysis. In: Biosensors and Bioelectronics. 1991 ; Vol. 6, No. 4. pp. 299-304.
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Amperometric fructose sensor based on direct bioelectrocatalysis. / Ikeda, Tokuji; Matsushita, Fumio; Senda, Mitsugi.

In: Biosensors and Bioelectronics, Vol. 6, No. 4, 01.01.1991, p. 299-304.

Research output: Contribution to journalArticle

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