TY - JOUR
T1 - Amperometric fructose sensor based on direct bioelectrocatalysis
AU - Ikeda, Tokuji
AU - Matsushita, Fumio
AU - Senda, Mitsugi
N1 - Funding Information:
This work was supportedi n part by a Grant-in Aid for Scientific Research( No. 01560150f)r om the Ministry of Education,S ciencea nd Culture of Japan.
PY - 1991
Y1 - 1991
N2 - Fructose dehydrogenase (EC 1.1.99.11) from bacterial membranes was immobilized on a carbon paste electrode by covering the enzyme layer with a dialysis membrane. The fructose dehydrogenase-modified carbon paste electrode showed a current response to d-fructose without the addition of any external electron transfer mediators. The current response was independent of the oxygen concentration in the solution. Steady-state currents were obtained when measured at fixed electrode potentials. The dependence of the steady-state current on the potential, the pH of the solution and the temperature was studied. On the basis of this investigation, it was shown that the fructose dehydrogenase-modified carbon paste electrode could be used as an unmediated amperometric fructose sensor. d-Fructose in fruits was measured using the present electrode. A method of eliminating the effect of l-ascorbic acid is also described.
AB - Fructose dehydrogenase (EC 1.1.99.11) from bacterial membranes was immobilized on a carbon paste electrode by covering the enzyme layer with a dialysis membrane. The fructose dehydrogenase-modified carbon paste electrode showed a current response to d-fructose without the addition of any external electron transfer mediators. The current response was independent of the oxygen concentration in the solution. Steady-state currents were obtained when measured at fixed electrode potentials. The dependence of the steady-state current on the potential, the pH of the solution and the temperature was studied. On the basis of this investigation, it was shown that the fructose dehydrogenase-modified carbon paste electrode could be used as an unmediated amperometric fructose sensor. d-Fructose in fruits was measured using the present electrode. A method of eliminating the effect of l-ascorbic acid is also described.
UR - http://www.scopus.com/inward/record.url?scp=0025870761&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025870761&partnerID=8YFLogxK
U2 - 10.1016/0956-5663(91)85015-O
DO - 10.1016/0956-5663(91)85015-O
M3 - Article
AN - SCOPUS:0025870761
VL - 6
SP - 299
EP - 304
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 4
ER -