Amphiregulin is not essential for induction of contact hypersensitivity

Akiko Yagami, Naoki Kajiwara, Keisuke Oboki, Tatsukuni Ohno, Hideaki Morita, Susan W. Sunnarborg, Ko Okumura, Hideoki Ogawa, Hirohisa Saito, Susumu Nakae

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3 Citations (Scopus)

Abstract

Background: Amphiregulin (AR) is expressed in Th2 cells, rather than Th1 cells, and plays an important role in Th2 cell/cytokine-mediated host defense against nematodes. We also found earlier that AR mRNA expression was strongly upregulated in inflamed tissue during Th2 cell/cytokine-mediated fluorescein isothiocyanate (FITC)-induced contact hypersensitivity (CHS), suggesting a contribution of AR to the induction of those responses. Methods: To elucidate the role of AR in the induction of FITC- or dinitrofluorobenzene (DNFB)-induced CHS, AR-deficient mice were sensitized and/or challenged with FITC or DNFB epicutaneously. The levels of FITCmediated skin dendritic cell (DC) migration and FITC-specific lymph node cell proliferation and cytokine production were assessed by flow cytometry, [3H]-thymidine incorporation and ELISA, respectively, after FITC sensitization. The degree of ear swelling, the activities of myeloperoxidase (MPO) and eosinophil peroxidase (EPO) in inflammatory sites and the levels of FITC-specific immunoglobulin (Ig) in sera were determined by histological analysis, colorimetric assay and ELISA, respectively, after FITC challenge. Results: DC migration and FITC-specific lymph node cell proliferation and cytokine production were normal in the AR-deficient mice. Ear swelling, tissue MPO and EPO activities and FITC-specific serum Ig levels were also similar in AR-deficient and -sufficient mice. Conclusions: Amphiregulin is not essential for the induction of FITC- or DNFB-induced CHS responses in mice.

Original languageEnglish
Pages (from-to)277-284
Number of pages8
JournalAllergology International
Volume59
Issue number3
DOIs
Publication statusPublished - 01-01-2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy

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  • Cite this

    Yagami, A., Kajiwara, N., Oboki, K., Ohno, T., Morita, H., Sunnarborg, S. W., Okumura, K., Ogawa, H., Saito, H., & Nakae, S. (2010). Amphiregulin is not essential for induction of contact hypersensitivity. Allergology International, 59(3), 277-284. https://doi.org/10.2332/allergolint.09-OA-0149