@inbook{9c642f4bf1da45de80a1747fc3efc950,
title = "Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures",
abstract = "Amyloid β (Aβ) peptides are the main component of the characteristic insoluble deposits in brain parenchyma and small blood vessels in the patients afflicted with Alzheimer{\textquoteright}s disease (AD) and cerebral amyloid angiopathy (CAA). These small peptides are attributed to the pathogenesis of both AD and CAA, suggesting an important index for disease stage and progression. In the brain tissue, Aβs are released mainly from neuronal cells into extracellular space. Here, we describe a step-by-step protocol to measure Aβs secreted from human pluripotent stem cell-derived neuronal cells.",
keywords = "Amyloid β (Aβ), Enzyme-Linked Immuno Sorbent Assay (ELISA), induced pluripotent stem cell (iPSC), Neuronal differentiation, β-Secretase, γ-Secretase",
author = "Hirotaka Watanabe and Rei Murakami and Hideyuki Okano",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC.",
year = "2022",
doi = "10.1007/7651\_2021\_407",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "209--217",
booktitle = "Methods in Molecular Biology",
}
