Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures

Hirotaka Watanabe; Rei Murakami; Hideyuki Okano

doi:10.1007/7651_2021_407

Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures

Hirotaka Watanabe
, Rei Murakami
, Hideyuki Okano

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Amyloid β (Aβ) peptides are the main component of the characteristic insoluble deposits in brain parenchyma and small blood vessels in the patients afflicted with Alzheimer’s disease (AD) and cerebral amyloid angiopathy (CAA). These small peptides are attributed to the pathogenesis of both AD and CAA, suggesting an important index for disease stage and progression. In the brain tissue, Aβs are released mainly from neuronal cells into extracellular space. Here, we describe a step-by-step protocol to measure Aβs secreted from human pluripotent stem cell-derived neuronal cells.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	209-217
Number of pages	9
DOIs	https://doi.org/10.1007/7651_2021_407
Publication status	Published - 2022
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2549
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

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10.1007/7651_2021_407

Cite this

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title = "Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures",

abstract = "Amyloid β (Aβ) peptides are the main component of the characteristic insoluble deposits in brain parenchyma and small blood vessels in the patients afflicted with Alzheimer{\textquoteright}s disease (AD) and cerebral amyloid angiopathy (CAA). These small peptides are attributed to the pathogenesis of both AD and CAA, suggesting an important index for disease stage and progression. In the brain tissue, Aβs are released mainly from neuronal cells into extracellular space. Here, we describe a step-by-step protocol to measure Aβs secreted from human pluripotent stem cell-derived neuronal cells.",

keywords = "Amyloid β (Aβ), Enzyme-Linked Immuno Sorbent Assay (ELISA), induced pluripotent stem cell (iPSC), Neuronal differentiation, β-Secretase, γ-Secretase",

author = "Hirotaka Watanabe and Rei Murakami and Hideyuki Okano",

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year = "2022",

doi = "10.1007/7651\_2021\_407",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

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AU - Watanabe, Hirotaka

AU - Murakami, Rei

AU - Okano, Hideyuki

PY - 2022

Y1 - 2022

N2 - Amyloid β (Aβ) peptides are the main component of the characteristic insoluble deposits in brain parenchyma and small blood vessels in the patients afflicted with Alzheimer’s disease (AD) and cerebral amyloid angiopathy (CAA). These small peptides are attributed to the pathogenesis of both AD and CAA, suggesting an important index for disease stage and progression. In the brain tissue, Aβs are released mainly from neuronal cells into extracellular space. Here, we describe a step-by-step protocol to measure Aβs secreted from human pluripotent stem cell-derived neuronal cells.

AB - Amyloid β (Aβ) peptides are the main component of the characteristic insoluble deposits in brain parenchyma and small blood vessels in the patients afflicted with Alzheimer’s disease (AD) and cerebral amyloid angiopathy (CAA). These small peptides are attributed to the pathogenesis of both AD and CAA, suggesting an important index for disease stage and progression. In the brain tissue, Aβs are released mainly from neuronal cells into extracellular space. Here, we describe a step-by-step protocol to measure Aβs secreted from human pluripotent stem cell-derived neuronal cells.

KW - Amyloid β (Aβ)

KW - Enzyme-Linked Immuno Sorbent Assay (ELISA)

KW - induced pluripotent stem cell (iPSC)

KW - Neuronal differentiation

KW - β-Secretase

KW - γ-Secretase

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M3 - Chapter

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AN - SCOPUS:85133532656

T3 - Methods in Molecular Biology

SP - 209

EP - 217

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Amyloid β (Aβ) ELISA of Human iPSC-Derived Neuronal Cultures

Abstract

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All Science Journal Classification (ASJC) codes

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