TY - JOUR
T1 - An Outbreak of USA300 Methicillin-Resistant Staphylococcus aureus among People with HIV in Japan
AU - Ikeuchi, Kazuhiko
AU - Adachi, Eisuke
AU - Sasaki, Takashi
AU - Suzuki, Masato
AU - Lim, Lay Ahyoung
AU - Saito, Makoto
AU - Koga, Michiko
AU - Tsutsumi, Takeya
AU - Kido, Yasutoshi
AU - Uehara, Yuki
AU - Yotsuyanagi, Hiroshi
N1 - Publisher Copyright:
© 2020 The Author(s). Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved.
PY - 2021/2/15
Y1 - 2021/2/15
N2 - Background: USA300 produces Panton-Valentin leucocidin (PVL) and is known as a predominant community-associated methicillin-resistant Staphylococcus aureus (MRSA) strain in the United States, but it was extremely rare in Japan. We report here an outbreak of USA300 in people with HIV (PWH) in Tokyo, Japan. Methods: We analyzed the cases of PVL-MRSA infection between 2010 and 2020 and screened for nasal colonization of PVL-MRSA in PWH who visited an HIV/AIDS referral hospital from December 2019 to March 2020. Whole-genome sequencing-based single nucleotide polymorphism (SNP) analysis was performed on these isolates. Results: During the study period, a total of 21 PVL-MRSA infections in 14 patients were identified after 2014. The carriage prevalence was 4.3% (12/277) and PVL-MRSA carriers were more likely to have sexually transmitted infections (STIs) within a year compared with patients who had neither a history of PVL-MRSA infection nor colonization (33.3% [4/12] vs 10.1% [26/258]; P =.03). SNP analysis showed that all 26 isolates were ST8-SCCmecIVa-USA300. Twenty-four isolates were closely related (≤100 SNP differences) and had the nonsynonymous SNPs associated with carbohydrate metabolism and antimicrobial tolerance. Conclusions: An outbreak of USA300 has been occurring among PWH in Tokyo and a history of STI was a risk of colonization.
AB - Background: USA300 produces Panton-Valentin leucocidin (PVL) and is known as a predominant community-associated methicillin-resistant Staphylococcus aureus (MRSA) strain in the United States, but it was extremely rare in Japan. We report here an outbreak of USA300 in people with HIV (PWH) in Tokyo, Japan. Methods: We analyzed the cases of PVL-MRSA infection between 2010 and 2020 and screened for nasal colonization of PVL-MRSA in PWH who visited an HIV/AIDS referral hospital from December 2019 to March 2020. Whole-genome sequencing-based single nucleotide polymorphism (SNP) analysis was performed on these isolates. Results: During the study period, a total of 21 PVL-MRSA infections in 14 patients were identified after 2014. The carriage prevalence was 4.3% (12/277) and PVL-MRSA carriers were more likely to have sexually transmitted infections (STIs) within a year compared with patients who had neither a history of PVL-MRSA infection nor colonization (33.3% [4/12] vs 10.1% [26/258]; P =.03). SNP analysis showed that all 26 isolates were ST8-SCCmecIVa-USA300. Twenty-four isolates were closely related (≤100 SNP differences) and had the nonsynonymous SNPs associated with carbohydrate metabolism and antimicrobial tolerance. Conclusions: An outbreak of USA300 has been occurring among PWH in Tokyo and a history of STI was a risk of colonization.
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U2 - 10.1093/infdis/jiaa651
DO - 10.1093/infdis/jiaa651
M3 - Article
C2 - 33057717
AN - SCOPUS:85102322154
SN - 0022-1899
VL - 223
SP - 610
EP - 620
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -
