Analyses of peripheral blood mononuclear cells in operational tolerance after pediatric living donor liver transplantation

Ying Li, Takaaki Koshiba, Atsushi Yoshizawa, Yukihide Yonekawa, Kosuke Masuda, Atsushi Ito, Mikiko Ueda, Takahide Mori, Hiroshi Kawamoto, Yoshimasa Tanaka, Shimon Sakaguchi, Nagahiro Minato, Kathryn J. Wood, Koichi Tanaka

Research output: Contribution to journalArticlepeer-review

206 Citations (Scopus)

Abstract

Operational tolerance (graft acceptance in an immuno-suppression (IS)-free environment) after living-donor liver transplantation (LDLT) could occur by our elective protocol in some patients. There is, nevertheless, no reliable parameter to monitor patients who may discontinue IS without a risk of rejection. To identify such parameters, we systemically phenotyped peripheral blood mononuclear cells from operationally tolerant patients. An increase was observed in the frequency of CD4+CD25high+ cells, B cells and Vδ1/Vδ2 γδ-cells ratio in operationally tolerant patients (Gr-tol; n = 12), compared with those from age-matched volunteers (Gr-vol; n = 24) or patients on IS (Gr-IS; n = 19). The frequency of NK cells was decreased in Gr-tol, compared with those in Gr-IS or Gr-vol. The frequency of NKT cells was decreased after LDLT, compared with that in Gr-vol. Although the contribution of those subsets to the tolerant state remains elusive, the results may provide important clues for reliable indicators of tolerance after LDLT.

Original languageEnglish
Pages (from-to)2118-2125
Number of pages8
JournalAmerican Journal of Transplantation
Volume4
Issue number12
DOIs
Publication statusPublished - 12-2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Transplantation
  • Pharmacology (medical)

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