TY - JOUR
T1 - Analysis of an alternative promoter that regulates tissue-specific expression of the human aromatic L-amino acid decarboxylase gene in cultured cell lines
AU - Sumi-Ichinose, C.
AU - Hasegawa, S.
AU - Ohtsuki, M.
AU - Nomura, H.
AU - Nomura, T.
AU - Hagino, Y.
AU - Fujita, K.
AU - Nagatsu, T.
PY - 1996
Y1 - 1996
N2 - The human aromatic L-amino acid decarboxylase (AADC) gene is transcribed in a tissue-specific manner by an alternative promoter. In this study using human cultured cell lines, we analyzed the alternative promoter that regulates tissue-specific expression of AADC. Neither neuronalnor nonneuronal-type mRNA of AADC was detected in HeLa cells, nonneuronal-type mRNA of AADC was expressed in HepG2 cells, and the neuronal-type was expressed in the SK-N-SH cell line. We examined the promoter activities located in 5′- and 3′-fianking regions of exon Nl and exon LI by transfection experiments. Plasmids containing 5′-flanking regions of exon LI, the shortest of which was 0.3kb, could promote specifically high expression of the reporter gene in HepG2 cells. On the other hand, plasmids containing 5'-flanking regions of exon Nl (3.6 kb to 0.5 kb) could promote the reporter gene expression not only in SK-N-SH cells but also in HeLa and HepG2. More enhanced expression were observed by transfection of plasmids containing parts of the first intron in these cell lines. Thus, these results suggest that the basal liver-specific promoter activity is located in the 5′-flanking region of exon LI and that the first intron may also be needed for enhanced expression rather than determination of cell-specificity.
AB - The human aromatic L-amino acid decarboxylase (AADC) gene is transcribed in a tissue-specific manner by an alternative promoter. In this study using human cultured cell lines, we analyzed the alternative promoter that regulates tissue-specific expression of AADC. Neither neuronalnor nonneuronal-type mRNA of AADC was detected in HeLa cells, nonneuronal-type mRNA of AADC was expressed in HepG2 cells, and the neuronal-type was expressed in the SK-N-SH cell line. We examined the promoter activities located in 5′- and 3′-fianking regions of exon Nl and exon LI by transfection experiments. Plasmids containing 5′-flanking regions of exon LI, the shortest of which was 0.3kb, could promote specifically high expression of the reporter gene in HepG2 cells. On the other hand, plasmids containing 5'-flanking regions of exon Nl (3.6 kb to 0.5 kb) could promote the reporter gene expression not only in SK-N-SH cells but also in HeLa and HepG2. More enhanced expression were observed by transfection of plasmids containing parts of the first intron in these cell lines. Thus, these results suggest that the basal liver-specific promoter activity is located in the 5′-flanking region of exon LI and that the first intron may also be needed for enhanced expression rather than determination of cell-specificity.
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U2 - 10.1007/BF01292612
DO - 10.1007/BF01292612
M3 - Article
C2 - 9026364
AN - SCOPUS:0030049462
SN - 0300-9564
VL - 103
SP - 1
EP - 15
JO - Journal of Neural Transmission
JF - Journal of Neural Transmission
IS - 1-2
ER -