Analysis of canine herpesvirus gB, gC and gD expressed by a recombinant vaccinia virus

X. Xuan, A. Kojima, T. Murata, T. Mikami, H. Otsuka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The genes encoding the canine herpesvirus (CHV) glycoprotein B (gB), gC and gD homologues have been reported already. However, products of these genes have not been identified yet. Previously, we have identified three CHV glycoproteins, gp145/112, gp80 and gp47 using a panel of monoclonal antibodies (MAbs). To determine which CHV glycoprotein corresponds to gB, gC or gD, the putative genes of gB, gC, and gD of CHV were inserted into the thymidine kinase gene of vaccinia virus LC16mO strain under the control of the early-late promoter for the vaccinia virus 7.5-kilodalton polypeptide. We demonstrated here that gp145/112, gp80 and gp47 were the translation products of the CHV gB, gC and gD genes, respectively. The antigenic authenticity of recombinant gB, gC and gD were confirmed by a panel of MAbs specific for each glycoprotein produced in CHV-infected cells. Immunization of mice with these recombinants produced high titers of neutralizing antibodies against CHV. These results suggest that recombinant vaccinia viruses expressing CHV gB, gC and gD may be useful to develop a vaccine to control CHV infection.

Original languageEnglish
Pages (from-to)1003-1010
Number of pages8
JournalArchives of Virology
Volume142
Issue number5
DOIs
Publication statusPublished - 06-06-1997

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Vaccinia virus
Herpesviridae
Canidae
Glycoproteins
Genes
Monoclonal Antibodies
Herpesviridae Infections
Thymidine Kinase
Neutralizing Antibodies
Canid herpesvirus 1 glycoprotein B
Immunization
Vaccines
Peptides

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Xuan, X. ; Kojima, A. ; Murata, T. ; Mikami, T. ; Otsuka, H. / Analysis of canine herpesvirus gB, gC and gD expressed by a recombinant vaccinia virus. In: Archives of Virology. 1997 ; Vol. 142, No. 5. pp. 1003-1010.
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Analysis of canine herpesvirus gB, gC and gD expressed by a recombinant vaccinia virus. / Xuan, X.; Kojima, A.; Murata, T.; Mikami, T.; Otsuka, H.

In: Archives of Virology, Vol. 142, No. 5, 06.06.1997, p. 1003-1010.

Research output: Contribution to journalArticle

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