Analysis of cell characterization using cell surface markers in the dermis

Yuichi Hasebe, Seiji Hasegawa, Noriko Hashimoto, Masashi Toyoda, Kenji Matsumoto, Akihiro Umezawa, Akiko Yagami, Kayoko Matsunaga, Hiroshi Mizutani, Satoru Nakata, Hirohiko Akamatsu

Research output: Contribution to journalArticle

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Abstract

Background: In recent years, it has been reported that stem cells exist in the mesenchymal tissues of the bone marrow and adipose. These stem cells are thought to express specific cell surface markers such as CD44, CD54, CD105, CD90, and CD271 and have been confirmed to be pluripotent. Furthermore, although it has been reported that stem cells are also present in the dermis, their cell surface markers and characteristics are not fully understood. Objective: To confirm the presence of stem cells in the dermis and their ability, employing the mesenchymal stem cell markers which have previously been reported as an indication. Methods: We analyzed the percentages of CD44 (+), CD54 (+), CD90 (+), CD105 (+), and CD271 (+) cells in the dermis of neonatal mice (HR-1 mouse) by performing immunostaining and FACS. Secondly, we isolated each type of marker-positive and -negative cells from dermal tissues and evaluated their proliferation potential and their ability to differentiate into adipocytes, osteoblasts, and chondrocytes. Results: According to the immunostaining and FACS results, we confirmed that stem cells that express CD44, CD54, CD90, CD105, and CD271 are present in the dermal tissues of neonatal mice. In addition, when we measured the proliferation and differentiation potentials of each type of marker-positive cells, it was revealed that cells expressing CD54 or CD271 have a high proliferation potential and are able to differentiate into adipocytes, osteoblasts, and chondrocytes. Conclusions: These results indicated that dermal tissues contain stem cells that express CD44, CD54, CD90, CD105, and CD271 which are stem cell markers. More precisely, it was suggested that both CD54 (+) and CD271 (+) stem cells have high proliferation and differentiation potentials.

Original languageEnglish
Pages (from-to)98-106
Number of pages9
JournalJournal of Dermatological Science
Volume62
Issue number2
DOIs
Publication statusPublished - 01-05-2011

Fingerprint

Dermis
Stem cells
Stem Cells
Tissue
Chondrocytes
Osteoblasts
Adipocytes
Skin
Cells
Mesenchymal Stromal Cells
Adapalene
Adipose Tissue
Bone Marrow
Bone

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology

Cite this

Hasebe, Y., Hasegawa, S., Hashimoto, N., Toyoda, M., Matsumoto, K., Umezawa, A., ... Akamatsu, H. (2011). Analysis of cell characterization using cell surface markers in the dermis. Journal of Dermatological Science, 62(2), 98-106. https://doi.org/10.1016/j.jdermsci.2011.01.012
Hasebe, Yuichi ; Hasegawa, Seiji ; Hashimoto, Noriko ; Toyoda, Masashi ; Matsumoto, Kenji ; Umezawa, Akihiro ; Yagami, Akiko ; Matsunaga, Kayoko ; Mizutani, Hiroshi ; Nakata, Satoru ; Akamatsu, Hirohiko. / Analysis of cell characterization using cell surface markers in the dermis. In: Journal of Dermatological Science. 2011 ; Vol. 62, No. 2. pp. 98-106.
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Hasebe, Y, Hasegawa, S, Hashimoto, N, Toyoda, M, Matsumoto, K, Umezawa, A, Yagami, A, Matsunaga, K, Mizutani, H, Nakata, S & Akamatsu, H 2011, 'Analysis of cell characterization using cell surface markers in the dermis', Journal of Dermatological Science, vol. 62, no. 2, pp. 98-106. https://doi.org/10.1016/j.jdermsci.2011.01.012

Analysis of cell characterization using cell surface markers in the dermis. / Hasebe, Yuichi; Hasegawa, Seiji; Hashimoto, Noriko; Toyoda, Masashi; Matsumoto, Kenji; Umezawa, Akihiro; Yagami, Akiko; Matsunaga, Kayoko; Mizutani, Hiroshi; Nakata, Satoru; Akamatsu, Hirohiko.

In: Journal of Dermatological Science, Vol. 62, No. 2, 01.05.2011, p. 98-106.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analysis of cell characterization using cell surface markers in the dermis

AU - Hasebe, Yuichi

AU - Hasegawa, Seiji

AU - Hashimoto, Noriko

AU - Toyoda, Masashi

AU - Matsumoto, Kenji

AU - Umezawa, Akihiro

AU - Yagami, Akiko

AU - Matsunaga, Kayoko

AU - Mizutani, Hiroshi

AU - Nakata, Satoru

AU - Akamatsu, Hirohiko

PY - 2011/5/1

Y1 - 2011/5/1

N2 - Background: In recent years, it has been reported that stem cells exist in the mesenchymal tissues of the bone marrow and adipose. These stem cells are thought to express specific cell surface markers such as CD44, CD54, CD105, CD90, and CD271 and have been confirmed to be pluripotent. Furthermore, although it has been reported that stem cells are also present in the dermis, their cell surface markers and characteristics are not fully understood. Objective: To confirm the presence of stem cells in the dermis and their ability, employing the mesenchymal stem cell markers which have previously been reported as an indication. Methods: We analyzed the percentages of CD44 (+), CD54 (+), CD90 (+), CD105 (+), and CD271 (+) cells in the dermis of neonatal mice (HR-1 mouse) by performing immunostaining and FACS. Secondly, we isolated each type of marker-positive and -negative cells from dermal tissues and evaluated their proliferation potential and their ability to differentiate into adipocytes, osteoblasts, and chondrocytes. Results: According to the immunostaining and FACS results, we confirmed that stem cells that express CD44, CD54, CD90, CD105, and CD271 are present in the dermal tissues of neonatal mice. In addition, when we measured the proliferation and differentiation potentials of each type of marker-positive cells, it was revealed that cells expressing CD54 or CD271 have a high proliferation potential and are able to differentiate into adipocytes, osteoblasts, and chondrocytes. Conclusions: These results indicated that dermal tissues contain stem cells that express CD44, CD54, CD90, CD105, and CD271 which are stem cell markers. More precisely, it was suggested that both CD54 (+) and CD271 (+) stem cells have high proliferation and differentiation potentials.

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Hasebe Y, Hasegawa S, Hashimoto N, Toyoda M, Matsumoto K, Umezawa A et al. Analysis of cell characterization using cell surface markers in the dermis. Journal of Dermatological Science. 2011 May 1;62(2):98-106. https://doi.org/10.1016/j.jdermsci.2011.01.012