Analysis of conserved ambisense sequences within GB virus C

Yutaka Kondo, Masashi Mizokami, Tatsunori Nakano, Takanobu Kato, Yasuhito Tanaka, Noboru Hirashima, Ryuzo Ueda, Mitoshi Kunimatsu, Makoto Sasaki, Kiyomi Yasuda, Shiro Iino

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

No analysis has been done of the ambisense of GB virus C (GBV-C). When the anti-genomes of 16 reported sequences of GBV-C were analyzed, nucleotide codons 1758 and 1402 within the antigenome were conserved initiation and stop codons, respectively. Nucleotide sequences were also determined within the same region of 22 GBV-C strains. The anti-genomes of 38 sequences were translated and a consensus sequence was determined. In accordance with the consensus sequence, overlapping peptides were synthesized and used for the detection of anti-synthetic peptide antibodies by ELISA. The positivity of antibodies among sera with GBV-C RNA was Significantly higher than among sera without GBV-C RNA (66.7% vs. 15.6%), regardless of the simultaneous presence of hepatitis B surface antigen or antibodies to hepatitis C virus (P < .05). These results indicated that a novel protein associated with GBV-C might be expressed from the ambisense of this virus.

Original languageEnglish
Pages (from-to)1185-1188
Number of pages4
JournalJournal of Infectious Diseases
Volume178
Issue number4
DOIs
Publication statusPublished - 1998

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

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