TY - JOUR
T1 - Analysis of conserved ambisense sequences within GB virus C
AU - Kondo, Yutaka
AU - Mizokami, Masashi
AU - Nakano, Tatsunori
AU - Kato, Takanobu
AU - Tanaka, Yasuhito
AU - Hirashima, Noboru
AU - Ueda, Ryuzo
AU - Kunimatsu, Mitoshi
AU - Sasaki, Makoto
AU - Yasuda, Kiyomi
AU - Iino, Shiro
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1998
Y1 - 1998
N2 - No analysis has been done of the ambisense of GB virus C (GBV-C). When the anti-genomes of 16 reported sequences of GBV-C were analyzed, nucleotide codons 1758 and 1402 within the antigenome were conserved initiation and stop codons, respectively. Nucleotide sequences were also determined within the same region of 22 GBV-C strains. The anti-genomes of 38 sequences were translated and a consensus sequence was determined. In accordance with the consensus sequence, overlapping peptides were synthesized and used for the detection of anti-synthetic peptide antibodies by ELISA. The positivity of antibodies among sera with GBV-C RNA was Significantly higher than among sera without GBV-C RNA (66.7% vs. 15.6%), regardless of the simultaneous presence of hepatitis B surface antigen or antibodies to hepatitis C virus (P < .05). These results indicated that a novel protein associated with GBV-C might be expressed from the ambisense of this virus.
AB - No analysis has been done of the ambisense of GB virus C (GBV-C). When the anti-genomes of 16 reported sequences of GBV-C were analyzed, nucleotide codons 1758 and 1402 within the antigenome were conserved initiation and stop codons, respectively. Nucleotide sequences were also determined within the same region of 22 GBV-C strains. The anti-genomes of 38 sequences were translated and a consensus sequence was determined. In accordance with the consensus sequence, overlapping peptides were synthesized and used for the detection of anti-synthetic peptide antibodies by ELISA. The positivity of antibodies among sera with GBV-C RNA was Significantly higher than among sera without GBV-C RNA (66.7% vs. 15.6%), regardless of the simultaneous presence of hepatitis B surface antigen or antibodies to hepatitis C virus (P < .05). These results indicated that a novel protein associated with GBV-C might be expressed from the ambisense of this virus.
UR - http://www.scopus.com/inward/record.url?scp=17344362091&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17344362091&partnerID=8YFLogxK
U2 - 10.1086/515681
DO - 10.1086/515681
M3 - Article
C2 - 9806057
AN - SCOPUS:17344362091
SN - 0022-1899
VL - 178
SP - 1185
EP - 1188
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -
