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Analysis of conserved ambisense sequences within GB virus C

  • Yutaka Kondo
  • , Masashi Mizokami
  • , Tatsunori Nakano
  • , Takanobu Kato
  • , Yasuhito Tanaka
  • , Noboru Hirashima
  • , Ryuzo Ueda
  • , Mitoshi Kunimatsu
  • , Makoto Sasaki
  • , Kiyomi Yasuda
  • , Shiro Iino

Research output: Contribution to journalArticlepeer-review

Abstract

No analysis has been done of the ambisense of GB virus C (GBV-C). When the anti-genomes of 16 reported sequences of GBV-C were analyzed, nucleotide codons 1758 and 1402 within the antigenome were conserved initiation and stop codons, respectively. Nucleotide sequences were also determined within the same region of 22 GBV-C strains. The anti-genomes of 38 sequences were translated and a consensus sequence was determined. In accordance with the consensus sequence, overlapping peptides were synthesized and used for the detection of anti-synthetic peptide antibodies by ELISA. The positivity of antibodies among sera with GBV-C RNA was Significantly higher than among sera without GBV-C RNA (66.7% vs. 15.6%), regardless of the simultaneous presence of hepatitis B surface antigen or antibodies to hepatitis C virus (P < .05). These results indicated that a novel protein associated with GBV-C might be expressed from the ambisense of this virus.

Original languageEnglish
Pages (from-to)1185-1188
Number of pages4
JournalJournal of Infectious Diseases
Volume178
Issue number4
DOIs
Publication statusPublished - 1998

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

  • General Medicine

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