Analysis of nitric oxide metabolism as a placental or maternal factor underlying the etiology of pre-eclampsia

Haruki Nishizawa, Kanako Pryor-Koishi, MacHiko Suzuki, Takema Kato, Takao Sekiya, Shin Tada, Hiroki Kurahashi, Yasuhiro Udagawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background:Defective nitric oxide (NO)-mediated vasodilation is widely regarded as an underlying cause of hypertension in pre-eclampsia, although there are also arguments against this hypothesis. Methods:We examined both the mRNA levels and the presence of a Glu298Asp substitution in the NO synthase (NOS) gene, as well as the NO metabolite concentration, in placentas and maternal sera from women with pre-eclampsia and in normotensive pregnant controls (25-40 vs. 24-41 weeks of gestation). Results:Pre-eclamptic and control placentas did not show any significant differences in their NO metabolite levels or their NOS expression levels as measured by quantitative RT-PCR. In addition, we did not find any association between pre-eclampsia and the occurrence of the Glu298Asp amino acid substitution in the NOS gene. In contrast, high maternal circulating NO metabolites were evident in severe pre-eclampsia (p < 0.0001). Although a positive correlation between circulating NO metabolites and blood pressure was not observed, uterine artery resistance measured by ultrasound was found to positively correlate with the maternal NO levels. Conclusions:Our current data suggest that an altered placental NOS pathway is unlikely to be the primary cause of pre-eclampsia and that the activation of this pathway is possibly in response to maternal symptoms.

Original languageEnglish
Pages (from-to)239-247
Number of pages9
JournalGynecologic and Obstetric Investigation
Volume68
Issue number4
DOIs
Publication statusPublished - 01-11-2009

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Pre-Eclampsia
Nitric Oxide
Mothers
Nitric Oxide Synthase
Placenta
Uterine Artery
Amino Acid Substitution
Vasodilation
Genes
Blood Pressure
Hypertension
Pregnancy
Polymerase Chain Reaction
Messenger RNA
Serum

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Nishizawa, Haruki ; Pryor-Koishi, Kanako ; Suzuki, MacHiko ; Kato, Takema ; Sekiya, Takao ; Tada, Shin ; Kurahashi, Hiroki ; Udagawa, Yasuhiro. / Analysis of nitric oxide metabolism as a placental or maternal factor underlying the etiology of pre-eclampsia. In: Gynecologic and Obstetric Investigation. 2009 ; Vol. 68, No. 4. pp. 239-247.
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Analysis of nitric oxide metabolism as a placental or maternal factor underlying the etiology of pre-eclampsia. / Nishizawa, Haruki; Pryor-Koishi, Kanako; Suzuki, MacHiko; Kato, Takema; Sekiya, Takao; Tada, Shin; Kurahashi, Hiroki; Udagawa, Yasuhiro.

In: Gynecologic and Obstetric Investigation, Vol. 68, No. 4, 01.11.2009, p. 239-247.

Research output: Contribution to journalArticle

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