Analysis of nitric oxide metabolism as a placental or maternal factor underlying the etiology of pre-eclampsia

Haruki Nishizawa, Kanako Pryor-Koishi, MacHiko Suzuki, Takema Kato, Takao Sekiya, Shin Tada, Hiroki Kurahashi, Yasuhiro Udagawa

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


Background:Defective nitric oxide (NO)-mediated vasodilation is widely regarded as an underlying cause of hypertension in pre-eclampsia, although there are also arguments against this hypothesis. Methods:We examined both the mRNA levels and the presence of a Glu298Asp substitution in the NO synthase (NOS) gene, as well as the NO metabolite concentration, in placentas and maternal sera from women with pre-eclampsia and in normotensive pregnant controls (25-40 vs. 24-41 weeks of gestation). Results:Pre-eclamptic and control placentas did not show any significant differences in their NO metabolite levels or their NOS expression levels as measured by quantitative RT-PCR. In addition, we did not find any association between pre-eclampsia and the occurrence of the Glu298Asp amino acid substitution in the NOS gene. In contrast, high maternal circulating NO metabolites were evident in severe pre-eclampsia (p < 0.0001). Although a positive correlation between circulating NO metabolites and blood pressure was not observed, uterine artery resistance measured by ultrasound was found to positively correlate with the maternal NO levels. Conclusions:Our current data suggest that an altered placental NOS pathway is unlikely to be the primary cause of pre-eclampsia and that the activation of this pathway is possibly in response to maternal symptoms.

Original languageEnglish
Pages (from-to)239-247
Number of pages9
JournalGynecologic and Obstetric Investigation
Issue number4
Publication statusPublished - 11-2009

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology


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