TY - GEN
T1 - Analysis of QT interval prolongation with heart failure by simulation of repolarization process
AU - Yamaguchi, T.
AU - Arafune, T.
AU - Sakuma, I.
AU - Watanabe, E.
AU - Shibata, N.
AU - Honjo, H.
AU - Kodama, I.
AU - Kamiya, K.
PY - 2005
Y1 - 2005
N2 - It has been postulated that action potential duration (APD) is prolonged and IKs, a slow component of delayed rectifier potassium current, decreases in heart failure. We have reported that QT interval is prolonged and expression weight of KCNE1, coding IKs channel, increases in patients with heart failure. Since it is known that increase in KCNE1 increases the maximum conductance of IKs channel, the mechanism of APD prolongation is not explained by over expression of KCNE1. In this study, we construct a cardiac membrane action potential simulation model based on the experimental data from Xenopus oocytes expressed KCNQ1 and KCNE1 to investigate the relationship between increase in KCNE1 and APD, In addition, we investigated effect of reduction in Ca2+-independent transient outward potassium current (Ito) on APD in heart failure. In simulation, APD at 5ng KCNE1 was 180.96ms, which was 4.63% longer than that at Ing KCNE1 (APD=172.96ms) and 55.9% longer than that at 0.2ng KCNE1 (APD=110.96ms). In the cases of KCNQ1 alone and 0.2ng KCNE1 coinjected, APD shortened as density of Ito decreased, and APD prolonged as density of Ito decreased in other cases. This study shows that increase in KCNE1 expression level makes maximum conductance of IKs channel increase and IKs channel open slowly and conductance of IKs channel decrease according to the APD time scale. Therefore increasing the KCNE1 expression level may prolong APD with this mechanism. This method of constructing a simulation model based on experiments helps to explain the relationship between potassium currents and QT interval prolongation.
AB - It has been postulated that action potential duration (APD) is prolonged and IKs, a slow component of delayed rectifier potassium current, decreases in heart failure. We have reported that QT interval is prolonged and expression weight of KCNE1, coding IKs channel, increases in patients with heart failure. Since it is known that increase in KCNE1 increases the maximum conductance of IKs channel, the mechanism of APD prolongation is not explained by over expression of KCNE1. In this study, we construct a cardiac membrane action potential simulation model based on the experimental data from Xenopus oocytes expressed KCNQ1 and KCNE1 to investigate the relationship between increase in KCNE1 and APD, In addition, we investigated effect of reduction in Ca2+-independent transient outward potassium current (Ito) on APD in heart failure. In simulation, APD at 5ng KCNE1 was 180.96ms, which was 4.63% longer than that at Ing KCNE1 (APD=172.96ms) and 55.9% longer than that at 0.2ng KCNE1 (APD=110.96ms). In the cases of KCNQ1 alone and 0.2ng KCNE1 coinjected, APD shortened as density of Ito decreased, and APD prolonged as density of Ito decreased in other cases. This study shows that increase in KCNE1 expression level makes maximum conductance of IKs channel increase and IKs channel open slowly and conductance of IKs channel decrease according to the APD time scale. Therefore increasing the KCNE1 expression level may prolong APD with this mechanism. This method of constructing a simulation model based on experiments helps to explain the relationship between potassium currents and QT interval prolongation.
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U2 - 10.1109/iembs.2005.1616199
DO - 10.1109/iembs.2005.1616199
M3 - Conference contribution
AN - SCOPUS:33846935509
SN - 0780387406
SN - 9780780387409
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 7309
EP - 7312
BT - Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
Y2 - 1 September 2005 through 4 September 2005
ER -