Analysis of T-Cell Receptor V Region Gene Usage of Cytotoxic T-Lymphocytes and Tumor-infiltrating Lymphocytes Derived from Human Autologous Gastric Signet Ring Cell Carcinomas

Hideyuki Ikeda, Noriyuki Sato, Akihiro Matsuura, Kokichi Kikuchi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

To determine the T-cell receptor (TCR) Vα/Vβ gene usage of the human autologous gastric tumor-specific cytotoxic T-lymphocytes (CTLs), we first established two pairs of tumor cell lines, HST2 and SSTW, from the malignant peritoneal effusions of signet ring cell carcinomas and their peripheral blood lymphocyte-derived tumor-specific CD8-positive CTL lines, TcHST2 and TcSSTW. TCR Vα/Vβ gene usage from these CTL was examined using the reversely transcribed-polymerase chain reaction method, demonstrating that the Vα 7, Vα 12, and Vβ 20 transcripts were commonly detected. The fact that repeated antigenic stimulation by mixed lymphocyte-autologous tumor cell cultures brought about the specific cytolysis and the restricted TCR usage of TCR Vα 7, Vα 12, and Vβ 20 strongly suggests that these TCR V region products participated in T-cell-cancer interaction. This restricted TCR V gene usage in the gastric signet ring cell carcinomas led us to examine further the frequency of TCR Vα/Vβ usage in 11 cases of in vivo tumor-infiltrating lymphocytes with this particular type of tumor. The data showed that Vα 7, Vα 12, Vβ 6, and Vβ 20 were also predominantly expressed among these tumor-infiltrating lymphocytes in vivo. However, it seemed that T-cells with these TCR V region products are not specific for the gastric signet ring cell carcinomas, since they also frequently infiltrate into noncancerous lesions, such as peptic ulcers. These data may suggest that T-cells with certain TCR Vα/Vβ products could preferentially infiltrate into the stomach tissue, while some of these T-cells may be cytotoxic to the neoplastic autologous tumor cells.

Original languageEnglish
Pages (from-to)3078-3084
Number of pages7
JournalCancer Research
Volume53
Issue number13
Publication statusPublished - 01-01-1993
Externally publishedYes

Fingerprint

Signet Ring Cell Carcinoma
Tumor-Infiltrating Lymphocytes
Cytotoxic T-Lymphocytes
T-Cell Antigen Receptor
Stomach
Genes
T-Lymphocytes
Neoplasms
CD8-Positive T-Lymphocytes
T-Cell Receptor Genes
Ascitic Fluid
Lymphocyte Activation
Tumor Cell Line
Peptic Ulcer
Cell Communication
Cell Culture Techniques
Lymphocytes
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

@article{234c42f12d8c40a3b96b5323a7240fb4,
title = "Analysis of T-Cell Receptor V Region Gene Usage of Cytotoxic T-Lymphocytes and Tumor-infiltrating Lymphocytes Derived from Human Autologous Gastric Signet Ring Cell Carcinomas",
abstract = "To determine the T-cell receptor (TCR) Vα/Vβ gene usage of the human autologous gastric tumor-specific cytotoxic T-lymphocytes (CTLs), we first established two pairs of tumor cell lines, HST2 and SSTW, from the malignant peritoneal effusions of signet ring cell carcinomas and their peripheral blood lymphocyte-derived tumor-specific CD8-positive CTL lines, TcHST2 and TcSSTW. TCR Vα/Vβ gene usage from these CTL was examined using the reversely transcribed-polymerase chain reaction method, demonstrating that the Vα 7, Vα 12, and Vβ 20 transcripts were commonly detected. The fact that repeated antigenic stimulation by mixed lymphocyte-autologous tumor cell cultures brought about the specific cytolysis and the restricted TCR usage of TCR Vα 7, Vα 12, and Vβ 20 strongly suggests that these TCR V region products participated in T-cell-cancer interaction. This restricted TCR V gene usage in the gastric signet ring cell carcinomas led us to examine further the frequency of TCR Vα/Vβ usage in 11 cases of in vivo tumor-infiltrating lymphocytes with this particular type of tumor. The data showed that Vα 7, Vα 12, Vβ 6, and Vβ 20 were also predominantly expressed among these tumor-infiltrating lymphocytes in vivo. However, it seemed that T-cells with these TCR V region products are not specific for the gastric signet ring cell carcinomas, since they also frequently infiltrate into noncancerous lesions, such as peptic ulcers. These data may suggest that T-cells with certain TCR Vα/Vβ products could preferentially infiltrate into the stomach tissue, while some of these T-cells may be cytotoxic to the neoplastic autologous tumor cells.",
author = "Hideyuki Ikeda and Noriyuki Sato and Akihiro Matsuura and Kokichi Kikuchi",
year = "1993",
month = "1",
day = "1",
language = "English",
volume = "53",
pages = "3078--3084",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "13",

}

Analysis of T-Cell Receptor V Region Gene Usage of Cytotoxic T-Lymphocytes and Tumor-infiltrating Lymphocytes Derived from Human Autologous Gastric Signet Ring Cell Carcinomas. / Ikeda, Hideyuki; Sato, Noriyuki; Matsuura, Akihiro; Kikuchi, Kokichi.

In: Cancer Research, Vol. 53, No. 13, 01.01.1993, p. 3078-3084.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analysis of T-Cell Receptor V Region Gene Usage of Cytotoxic T-Lymphocytes and Tumor-infiltrating Lymphocytes Derived from Human Autologous Gastric Signet Ring Cell Carcinomas

AU - Ikeda, Hideyuki

AU - Sato, Noriyuki

AU - Matsuura, Akihiro

AU - Kikuchi, Kokichi

PY - 1993/1/1

Y1 - 1993/1/1

N2 - To determine the T-cell receptor (TCR) Vα/Vβ gene usage of the human autologous gastric tumor-specific cytotoxic T-lymphocytes (CTLs), we first established two pairs of tumor cell lines, HST2 and SSTW, from the malignant peritoneal effusions of signet ring cell carcinomas and their peripheral blood lymphocyte-derived tumor-specific CD8-positive CTL lines, TcHST2 and TcSSTW. TCR Vα/Vβ gene usage from these CTL was examined using the reversely transcribed-polymerase chain reaction method, demonstrating that the Vα 7, Vα 12, and Vβ 20 transcripts were commonly detected. The fact that repeated antigenic stimulation by mixed lymphocyte-autologous tumor cell cultures brought about the specific cytolysis and the restricted TCR usage of TCR Vα 7, Vα 12, and Vβ 20 strongly suggests that these TCR V region products participated in T-cell-cancer interaction. This restricted TCR V gene usage in the gastric signet ring cell carcinomas led us to examine further the frequency of TCR Vα/Vβ usage in 11 cases of in vivo tumor-infiltrating lymphocytes with this particular type of tumor. The data showed that Vα 7, Vα 12, Vβ 6, and Vβ 20 were also predominantly expressed among these tumor-infiltrating lymphocytes in vivo. However, it seemed that T-cells with these TCR V region products are not specific for the gastric signet ring cell carcinomas, since they also frequently infiltrate into noncancerous lesions, such as peptic ulcers. These data may suggest that T-cells with certain TCR Vα/Vβ products could preferentially infiltrate into the stomach tissue, while some of these T-cells may be cytotoxic to the neoplastic autologous tumor cells.

AB - To determine the T-cell receptor (TCR) Vα/Vβ gene usage of the human autologous gastric tumor-specific cytotoxic T-lymphocytes (CTLs), we first established two pairs of tumor cell lines, HST2 and SSTW, from the malignant peritoneal effusions of signet ring cell carcinomas and their peripheral blood lymphocyte-derived tumor-specific CD8-positive CTL lines, TcHST2 and TcSSTW. TCR Vα/Vβ gene usage from these CTL was examined using the reversely transcribed-polymerase chain reaction method, demonstrating that the Vα 7, Vα 12, and Vβ 20 transcripts were commonly detected. The fact that repeated antigenic stimulation by mixed lymphocyte-autologous tumor cell cultures brought about the specific cytolysis and the restricted TCR usage of TCR Vα 7, Vα 12, and Vβ 20 strongly suggests that these TCR V region products participated in T-cell-cancer interaction. This restricted TCR V gene usage in the gastric signet ring cell carcinomas led us to examine further the frequency of TCR Vα/Vβ usage in 11 cases of in vivo tumor-infiltrating lymphocytes with this particular type of tumor. The data showed that Vα 7, Vα 12, Vβ 6, and Vβ 20 were also predominantly expressed among these tumor-infiltrating lymphocytes in vivo. However, it seemed that T-cells with these TCR V region products are not specific for the gastric signet ring cell carcinomas, since they also frequently infiltrate into noncancerous lesions, such as peptic ulcers. These data may suggest that T-cells with certain TCR Vα/Vβ products could preferentially infiltrate into the stomach tissue, while some of these T-cells may be cytotoxic to the neoplastic autologous tumor cells.

UR - http://www.scopus.com/inward/record.url?scp=0027295342&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027295342&partnerID=8YFLogxK

M3 - Article

C2 - 8391386

AN - SCOPUS:0027295342

VL - 53

SP - 3078

EP - 3084

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 13

ER -