Analysis of the Alternative Promoters that Regulate Tissue‐Specific Expression of Human Aromatic l‐Amino Acid Decarboxylase

Chiho Ichinose, Seiko Hasegawa, Hiroshi Ichinose, Hirohide Sawada, Kazuto Kobayashi, Masao Sakai, Tetsuya Fujii, Hiroko Nomura, Takahide Nomura, Ikuko Nagatsu, Yasumichi Hagino, Keisuke Fujita, Toshiharu Nagatsu

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Abstract: Previously we identified two alternative first exons (exon N1 and exon L1) coding for 5′ untranslated regions of human aromatic l‐amino acid decarboxylase (AADC) and found that their alternative usage produced two types of mRNAs in a tissue‐specific manner. To determine the cis‐acting element regulating the tissue‐specific expression of human AADC, we produced three kinds of transgenic mice harboring 5′ flanking regions of the human AADC gene fused to the bacterial chloramphenicol acetyltransferase (CAT) gene. The transgene termed ACA contained −7.0 kb to −30 bp in exon N1, including the entire exon L1; ACN contained −3.6 kb to −30 bp in exon N1; and ACL contained −2.8 kb to −42 bp in exon L1. The ACA transgenic mice expressed CAT at extremely high levels in peripheral nonneuronal tissues, such as pancreas, liver, kidney, small intestine, and colon, that contained endogenous high AADC activity, whereas CAT immunoreactivity was not detected in either catecholaminergic or serotonergic neurons in the CNS. Thus, it was suggested that the ACA transgene contained the major part of cis‐regulatory elements for the expression of AADC in peripheral nonneuronal tissues. On the other hand, the ACN transgenic mice moderately expressed CAT in various tissues except for the lung and liver, and the ACL transgenic mice showed moderate CAT expression only in the kidney.

Original languageEnglish
Pages (from-to)514-524
Number of pages11
JournalJournal of Neurochemistry
Volume64
Issue number2
DOIs
Publication statusPublished - 01-01-1995

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Carboxy-Lyases
Carboxylic acids
Chloramphenicol O-Acetyltransferase
Exons
Acids
Transgenic Mice
Tissue
Transgenes
Liver
Genes
Kidney
Serotonergic Neurons
5' Flanking Region
5' Untranslated Regions
Neurons
Small Intestine
Pancreas
Colon
Lung
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Ichinose, Chiho ; Hasegawa, Seiko ; Ichinose, Hiroshi ; Sawada, Hirohide ; Kobayashi, Kazuto ; Sakai, Masao ; Fujii, Tetsuya ; Nomura, Hiroko ; Nomura, Takahide ; Nagatsu, Ikuko ; Hagino, Yasumichi ; Fujita, Keisuke ; Nagatsu, Toshiharu. / Analysis of the Alternative Promoters that Regulate Tissue‐Specific Expression of Human Aromatic l‐Amino Acid Decarboxylase. In: Journal of Neurochemistry. 1995 ; Vol. 64, No. 2. pp. 514-524.
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abstract = "Abstract: Previously we identified two alternative first exons (exon N1 and exon L1) coding for 5′ untranslated regions of human aromatic l‐amino acid decarboxylase (AADC) and found that their alternative usage produced two types of mRNAs in a tissue‐specific manner. To determine the cis‐acting element regulating the tissue‐specific expression of human AADC, we produced three kinds of transgenic mice harboring 5′ flanking regions of the human AADC gene fused to the bacterial chloramphenicol acetyltransferase (CAT) gene. The transgene termed ACA contained −7.0 kb to −30 bp in exon N1, including the entire exon L1; ACN contained −3.6 kb to −30 bp in exon N1; and ACL contained −2.8 kb to −42 bp in exon L1. The ACA transgenic mice expressed CAT at extremely high levels in peripheral nonneuronal tissues, such as pancreas, liver, kidney, small intestine, and colon, that contained endogenous high AADC activity, whereas CAT immunoreactivity was not detected in either catecholaminergic or serotonergic neurons in the CNS. Thus, it was suggested that the ACA transgene contained the major part of cis‐regulatory elements for the expression of AADC in peripheral nonneuronal tissues. On the other hand, the ACN transgenic mice moderately expressed CAT in various tissues except for the lung and liver, and the ACL transgenic mice showed moderate CAT expression only in the kidney.",
author = "Chiho Ichinose and Seiko Hasegawa and Hiroshi Ichinose and Hirohide Sawada and Kazuto Kobayashi and Masao Sakai and Tetsuya Fujii and Hiroko Nomura and Takahide Nomura and Ikuko Nagatsu and Yasumichi Hagino and Keisuke Fujita and Toshiharu Nagatsu",
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Ichinose, C, Hasegawa, S, Ichinose, H, Sawada, H, Kobayashi, K, Sakai, M, Fujii, T, Nomura, H, Nomura, T, Nagatsu, I, Hagino, Y, Fujita, K & Nagatsu, T 1995, 'Analysis of the Alternative Promoters that Regulate Tissue‐Specific Expression of Human Aromatic l‐Amino Acid Decarboxylase', Journal of Neurochemistry, vol. 64, no. 2, pp. 514-524. https://doi.org/10.1046/j.1471-4159.1995.64020514.x

Analysis of the Alternative Promoters that Regulate Tissue‐Specific Expression of Human Aromatic l‐Amino Acid Decarboxylase. / Ichinose, Chiho; Hasegawa, Seiko; Ichinose, Hiroshi; Sawada, Hirohide; Kobayashi, Kazuto; Sakai, Masao; Fujii, Tetsuya; Nomura, Hiroko; Nomura, Takahide; Nagatsu, Ikuko; Hagino, Yasumichi; Fujita, Keisuke; Nagatsu, Toshiharu.

In: Journal of Neurochemistry, Vol. 64, No. 2, 01.01.1995, p. 514-524.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analysis of the Alternative Promoters that Regulate Tissue‐Specific Expression of Human Aromatic l‐Amino Acid Decarboxylase

AU - Ichinose, Chiho

AU - Hasegawa, Seiko

AU - Ichinose, Hiroshi

AU - Sawada, Hirohide

AU - Kobayashi, Kazuto

AU - Sakai, Masao

AU - Fujii, Tetsuya

AU - Nomura, Hiroko

AU - Nomura, Takahide

AU - Nagatsu, Ikuko

AU - Hagino, Yasumichi

AU - Fujita, Keisuke

AU - Nagatsu, Toshiharu

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Abstract: Previously we identified two alternative first exons (exon N1 and exon L1) coding for 5′ untranslated regions of human aromatic l‐amino acid decarboxylase (AADC) and found that their alternative usage produced two types of mRNAs in a tissue‐specific manner. To determine the cis‐acting element regulating the tissue‐specific expression of human AADC, we produced three kinds of transgenic mice harboring 5′ flanking regions of the human AADC gene fused to the bacterial chloramphenicol acetyltransferase (CAT) gene. The transgene termed ACA contained −7.0 kb to −30 bp in exon N1, including the entire exon L1; ACN contained −3.6 kb to −30 bp in exon N1; and ACL contained −2.8 kb to −42 bp in exon L1. The ACA transgenic mice expressed CAT at extremely high levels in peripheral nonneuronal tissues, such as pancreas, liver, kidney, small intestine, and colon, that contained endogenous high AADC activity, whereas CAT immunoreactivity was not detected in either catecholaminergic or serotonergic neurons in the CNS. Thus, it was suggested that the ACA transgene contained the major part of cis‐regulatory elements for the expression of AADC in peripheral nonneuronal tissues. On the other hand, the ACN transgenic mice moderately expressed CAT in various tissues except for the lung and liver, and the ACL transgenic mice showed moderate CAT expression only in the kidney.

AB - Abstract: Previously we identified two alternative first exons (exon N1 and exon L1) coding for 5′ untranslated regions of human aromatic l‐amino acid decarboxylase (AADC) and found that their alternative usage produced two types of mRNAs in a tissue‐specific manner. To determine the cis‐acting element regulating the tissue‐specific expression of human AADC, we produced three kinds of transgenic mice harboring 5′ flanking regions of the human AADC gene fused to the bacterial chloramphenicol acetyltransferase (CAT) gene. The transgene termed ACA contained −7.0 kb to −30 bp in exon N1, including the entire exon L1; ACN contained −3.6 kb to −30 bp in exon N1; and ACL contained −2.8 kb to −42 bp in exon L1. The ACA transgenic mice expressed CAT at extremely high levels in peripheral nonneuronal tissues, such as pancreas, liver, kidney, small intestine, and colon, that contained endogenous high AADC activity, whereas CAT immunoreactivity was not detected in either catecholaminergic or serotonergic neurons in the CNS. Thus, it was suggested that the ACA transgene contained the major part of cis‐regulatory elements for the expression of AADC in peripheral nonneuronal tissues. On the other hand, the ACN transgenic mice moderately expressed CAT in various tissues except for the lung and liver, and the ACL transgenic mice showed moderate CAT expression only in the kidney.

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U2 - 10.1046/j.1471-4159.1995.64020514.x

DO - 10.1046/j.1471-4159.1995.64020514.x

M3 - Article

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SP - 514

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JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

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