Analysis of the vasopressin system and water regulation in genetically polydipsic mice

Yuko Yambe, Yasuko Watanabe-Tomita, Satoshi Kakiya, Hisashi Yokoi, Hiroshi Nagasaki, Hiroshi Arima, Takashi Murase, Hiromitsu Yuasa, Kunikazu Kondo, Hiroshi Yamashita, Yutaka Oiso

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Polydipsic mice, STR/N, which show extreme polydipsia and polyuria, were discovered in 1958. In the STR/N, urine outputs are much higher than in control mice. The possibility of an abnormal regulation of the arginine vasopressin (AVP) system, or an abnormality in the renal susceptibility to AVP, should be considered. In this study we investigated the AVP system and water regulation in STR/N. We sequenced the AVP and the AVP V2-receptor genes of the STR/N by direct sequencing. No mutation was found in either of them. AVP gene expression examined by in situ hybridization and plasma sodium in 8-wk-old STR/N was significantly lower than in control mice, whereas it was significantly higher at 20 wk. Renal sensitivity to injected AVP was attenuated in 20-wk-old STR/N. The suppression of AVP synthesis due to excessive water retention in 8-wk-old STR/N suggests that polydipsia may be the primary cause in this strain. The 20-wk-old STR/N became dehydrated with the acceleration of AVP synthesis, which might have resulted from secondary desensitization to AVP.

Original languageEnglish
Pages (from-to)E189-E194
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume278
Issue number2 41-2
DOIs
Publication statusPublished - 02-2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

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