Anatomical substrates for the discriminative stimulus effects of methamphetamine in rats

Akira Nakajima, Kiyofumi Yamada, Jue He, Nan Zeng, Atsumi Nitta, Toshitaka Nabeshima

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Methamphetamine is a psychostimulant drug acting on central monoaminergic neurons to produce both acute psychomotor stimulation and long-lasting behavioral effects including addiction and psychosis. Drug discrimination procedures have been particularly useful in characterizing subjective effects of addictive drugs. In the present study, to identify potential anatomical substrates for the discriminative stimulus effects of methamphetamine, we investigated the drug discrimination-associated Fos expression in Sprague-Dawley rats trained to discriminate methamphetamine from saline under a two-lever fixed ratio 20 (FR-20) schedule of food reinforcement. The rats that fulfilled the criteria for learning the discrimination were anesthetized and perfused 2 h after the drug discrimination test, and Fos immunoreactivity was examined in 15 brain regions. Fos expression in the brains of rats that discriminate methamphetamine from saline was significantly increased in the nucleus accumbens (NAc) and the ventral tegmental area (VTA), but not in other areas including the cerebral cortex, caudate putamen, substantia nigra, hippocampus, amygdala and habenulla, as compared with the expression in control rats that were maintained under the FR-20 schedule. The present findings suggest a role for the VTA and NAc as possible neuronal substrates in the discriminative stimulus effects of methamphetamine.

Original languageEnglish
Pages (from-to)308-317
Number of pages10
JournalJournal of Neurochemistry
Volume91
Issue number2
DOIs
Publication statusPublished - 01-10-2004
Externally publishedYes

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Methamphetamine
Rats
Substrates
Ventral Tegmental Area
Pharmaceutical Preparations
Nucleus Accumbens
Brain
Tegmentum Mesencephali
Rat control
Discrimination Learning
Reinforcement Schedule
Putamen
Substantia Nigra
Amygdala
Cerebral Cortex
Psychotic Disorders
Neurons
Sprague Dawley Rats
Hippocampus
Appointments and Schedules

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Nakajima, Akira ; Yamada, Kiyofumi ; He, Jue ; Zeng, Nan ; Nitta, Atsumi ; Nabeshima, Toshitaka. / Anatomical substrates for the discriminative stimulus effects of methamphetamine in rats. In: Journal of Neurochemistry. 2004 ; Vol. 91, No. 2. pp. 308-317.
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Anatomical substrates for the discriminative stimulus effects of methamphetamine in rats. / Nakajima, Akira; Yamada, Kiyofumi; He, Jue; Zeng, Nan; Nitta, Atsumi; Nabeshima, Toshitaka.

In: Journal of Neurochemistry, Vol. 91, No. 2, 01.10.2004, p. 308-317.

Research output: Contribution to journalArticle

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